What materials are provided with Universal Plus Total RNA-Seq with NuQuant?
The Universal Plus Total RNA-Seq with NuQuant kit bundle (Part No. 9156, 9157, 9158) provides all necessary buffers, primers and enzymes for fragmentation, cDNA synthesis, library construction, AnyDeplete and NuQuant. SPRI purification beads and EvaGreen are not included.
What is the concentration of the control RNA?
The control RNA (K562 total RNA), included only with the 24 reaction kit, is provided at a concentration of 1 μg/μL.
What equipment is required or will be useful?
Required equipment includes a microcentrifuge, pipettes, vortexer, a thermal cycler, a magnetic plate for 0.2 mL tubes, strips, or plates and a spectrophotometer or fluorometer. An Agilent Bioanalyzer or Fragment Analyzer may also be useful for optional analytical tests. A comprehensive list of required and recommended equipment can be found in Section II.B.
Can this system be used with other library preparation workflows?
Universal Plus Total RNA-Seq is an end-to-end solution designed to generate libraries for Illumina sequencing starting from total RNA and has not been tested with alternative library preparation systems.