Infinite^® 200 PRO Configurations

This Technical Note describes optimized test set-ups, instrument settings and the statistical equations that were used to determine the maximum instrument sensitivity of the Infinite F200 PRO filter-based microplate reader and the Infinite M200 PRO Quad4 Monochromators-based microplate reader for flash and glow luminescence.

The BacTiter-Glo Microbial Cell Viability Assay was tested on the Infinite F200 PRO filter-based multimode reader, using the instrument’s highly sensitive luminescence module.

This note describes the Dual Luciferase Reporter Assay performance using Tecan’s Infinite 200 PRO multimode reader equipped with a two channel injector.

Dynamic interactions between proteins are key mediators of multiple cellular functions and are involved in almost all biological processes. Accordingly, they are valuable targets for the development of novel drug therapies. In this context, bioluminescence resonance energy transfer (BRET), developed by Promega, is a well-established detection technology for the investigation of protein-protein interactions in living cells. However, its application has so far been somewhat hampered by its limited dynamic range and sensitivity.

The NanoQuant Plate from Tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. Additionally, the efficiency of the nucleic acid labeling with dyes can be easily determined with this tool.

In this note we describe the use of Tecan’s Infinite F200 PRO and Infinite M200 PRO instruments for easy and sensitive protein quantification using different absorbance-based assays.

The isolation of adult stem cells results in very low amounts of cells due to their rare incidence in already formed tissues and organs. Here we describe the preparation and evaluation of RNA samples from a novel population of mouse keratinocyte stem cells marked by Lgr5+ expression. We used tools specifically designed for handling and measuring low RNA quantities including RNA purification and the subsequent quantification with the Infinite M200 PRO.

Tecan´s NanoQuant detection technology has been developed to facilitate the quantification of biomolecules in small-volume samples. The present note describes the implementation of the NanoQuant Plate for protein measurements with regard to essential assay parameters such as linearity, uniformity, and reproducibility.

This application note describes the detection of S. aureus growth using Tecan’s Infinite 200 PRO microplate reader to perform 600 nm absorbance measurements. The influence of assay parameter and instrument settings on data quality is discussed.

This note describes the use of the fluorescence microplate reader Tecan Infinite F200 PRO or M200 PRO, for detection of as lias 20 pg dsDNA in a 200 μl assay volume.

One of the most used methods for determining nucleic acid concentrations is based on measuring the optical density of a sample at 260 nm (OD260). According to the Lambert-Beer law, the amount of absorbed light is proportional to the concentration of the sample, and to the pathlength the light has to pass through when going through the sample.

The NanoQuant Plate from Tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. Additionally, the efficiency of the nucleic acid labeling with dyes can be easily determined with this tool.

In this note we describe the use of Tecan’s Infinite F200 PRO and Infinite M200 PRO instruments for easy and sensitive protein quantification using different absorbance-based assays.

The isolation of adult stem cells results in very low amounts of cells due to their rare incidence in already formed tissues and organs. Here we describe the preparation and evaluation of RNA samples from a novel population of mouse keratinocyte stem cells marked by Lgr5+ expression. We used tools specifically designed for handling and measuring low RNA quantities including RNA purification and the subsequent quantification with the Infinite M200 PRO.

Tecan´s NanoQuant detection technology has been developed to facilitate the quantification of biomolecules in small-volume samples. The present note describes the implementation of the NanoQuant Plate for protein measurements with regard to essential assay parameters such as linearity, uniformity, and reproducibility.

This application note describes the detection of S. aureus growth using Tecan’s Infinite 200 PRO microplate reader to perform 600 nm absorbance measurements. The influence of assay parameter and instrument settings on data quality is discussed.

This note describes the use of the fluorescence microplate reader Tecan Infinite F200 PRO or M200 PRO, for detection of as lias 20 pg dsDNA in a 200 μl assay volume.

This application note describes the use of the Infinite® F200 PRO in combination with a commercially available ORAC assay kit, using different beverages as antioxidant samples.

One of the most used methods for determining nucleic acid concentrations is based on measuring the optical density of a sample at 260 nm (OD260). According to the Lambert-Beer law, the amount of absorbed light is proportional to the concentration of the sample, and to the pathlength the light has to pass through when going through the sample.

The NanoQuant Plate from Tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. Additionally, the efficiency of the nucleic acid labeling with dyes can be easily determined with this tool.

In this note we describe the use of Tecan’s Infinite F200 PRO and Infinite M200 PRO instruments for easy and sensitive protein quantification using different absorbance-based assays.

The isolation of adult stem cells results in very low amounts of cells due to their rare incidence in already formed tissues and organs. Here we describe the preparation and evaluation of RNA samples from a novel population of mouse keratinocyte stem cells marked by Lgr5+ expression. We used tools specifically designed for handling and measuring low RNA quantities including RNA purification and the subsequent quantification with the Infinite M200 PRO.

Tecan´s NanoQuant detection technology has been developed to facilitate the quantification of biomolecules in small-volume samples. The present note describes the implementation of the NanoQuant Plate for protein measurements with regard to essential assay parameters such as linearity, uniformity, and reproducibility.

This application note describes the detection of S. aureus growth using Tecan’s Infinite 200 PRO microplate reader to perform 600 nm absorbance measurements. The influence of assay parameter and instrument settings on data quality is discussed.

This note describes the use of the fluorescence microplate reader Tecan Infinite F200 PRO or M200 PRO, for detection of as lias 20 pg dsDNA in a 200 μl assay volume.

In this Application Note the three assay systems introduced above (i.e. MTT assay, CellTiter96 AQueous One Solution Cell Proliferation assay (MTS) and CellTiter-Blue Cell Viability Assay (resazurin) are compared using the human cholangiocarcinoma cell line CCLP-1. The compatibility of these assay systems with Tecan´s Infinite M200 PRO Quad4 Monochromators-based multimode reader is demonstrated.

The BacTiter-Glo Microbial Cell Viability Assay was tested on the Infinite F200 PRO filter-based multimode reader, using the instrument’s highly sensitive luminescence module.

This note describes the Dual Luciferase Reporter Assay performance using Tecan’s Infinite 200 PRO multimode reader equipped with a two channel injector.

Dynamic interactions between proteins are key mediators of multiple cellular functions and are involved in almost all biological processes. Accordingly, they are valuable targets for the development of novel drug therapies. In this context, bioluminescence resonance energy transfer (BRET), developed by Promega, is a well-established detection technology for the investigation of protein-protein interactions in living cells. However, its application has so far been somewhat hampered by its limited dynamic range and sensitivity.

One of the most used methods for determining nucleic acid concentrations is based on measuring the optical density of a sample at 260 nm (OD260). According to the Lambert-Beer law, the amount of absorbed light is proportional to the concentration of the sample, and to the pathlength the light has to pass through when going through the sample.

The NanoQuant Plate from Tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. Additionally, the efficiency of the nucleic acid labeling with dyes can be easily determined with this tool.

In this note we describe the use of Tecan’s Infinite F200 PRO and Infinite M200 PRO instruments for easy and sensitive protein quantification using different absorbance-based assays.

The isolation of adult stem cells results in very low amounts of cells due to their rare incidence in already formed tissues and organs. Here we describe the preparation and evaluation of RNA samples from a novel population of mouse keratinocyte stem cells marked by Lgr5+ expression. We used tools specifically designed for handling and measuring low RNA quantities including RNA purification and the subsequent quantification with the Infinite M200 PRO.

Tecan´s NanoQuant detection technology has been developed to facilitate the quantification of biomolecules in small-volume samples. The present note describes the implementation of the NanoQuant Plate for protein measurements with regard to essential assay parameters such as linearity, uniformity, and reproducibility.

This application note describes the detection of S. aureus growth using Tecan’s Infinite 200 PRO microplate reader to perform 600 nm absorbance measurements. The influence of assay parameter and instrument settings on data quality is discussed.

This note describes the use of the fluorescence microplate reader Tecan Infinite F200 PRO or M200 PRO, for detection of as lias 20 pg dsDNA in a 200 μl assay volume.

This application note describes the use of the Infinite® F200 PRO in combination with a commercially available ORAC assay kit, using different beverages as antioxidant samples.

In this Application Note the three assay systems introduced above (i.e. MTT assay, CellTiter96 AQueous One Solution Cell Proliferation assay (MTS) and CellTiter-Blue Cell Viability Assay (resazurin) are compared using the human cholangiocarcinoma cell line CCLP-1. The compatibility of these assay systems with Tecan´s Infinite M200 PRO Quad4 Monochromators-based multimode reader is demonstrated.

The present application note summarizes the results of experiments with the HTRF (Homogeneous Time-Resolved Fluorescence, Cisbio Bioassays) technology on Infinite F200 PRO.

The present application note summarizes the results of experiments with the HTRF (Homogeneous Time-Resolved Fluorescence, Cisbio Bioassays) technology on Infinite F200 PRO.

The Transcreener ADP2 TR-FRET Red Assay is intended for the detection of ADP production by any kinase or ATPase, and has been developed specifically for high throughput screening applications. The assay is based on the competitive binding of ADP to a monoclonal antibody-terbium conjugate, and uses a homogeneous TR-FRET detection mechanism.

This Technical Note describes optimized test set-ups, instrument settings and the statistical equations that were used to determine the maximum instrument sensitivity of the Infinite F200 PRO filter-based microplate reader and the Infinite M200 PRO Quad4 Monochromators-based microplate reader for flash and glow luminescence.

The BacTiter-Glo Microbial Cell Viability Assay was tested on the Infinite F200 PRO filter-based multimode reader, using the instrument’s highly sensitive luminescence module.

This note describes the Dual Luciferase Reporter Assay performance using Tecan’s Infinite 200 PRO multimode reader equipped with a two channel injector.

Dynamic interactions between proteins are key mediators of multiple cellular functions and are involved in almost all biological processes. Accordingly, they are valuable targets for the development of novel drug therapies. In this context, bioluminescence resonance energy transfer (BRET), developed by Promega, is a well-established detection technology for the investigation of protein-protein interactions in living cells. However, its application has so far been somewhat hampered by its limited dynamic range and sensitivity.

One of the most used methods for determining nucleic acid concentrations is based on measuring the optical density of a sample at 260 nm (OD260). According to the Lambert-Beer law, the amount of absorbed light is proportional to the concentration of the sample, and to the pathlength the light has to pass through when going through the sample.