D300e Digital Dispenser

From small molecules in DMSO to biomolecules in surfactant-containing aqueous solutions, this convenient benchtop solution allows rapid delivery of any volume to any well.

Requiring almost no set-up time, it uses disposable Dispenseheads to minimize dead volumes and virtually eliminate the risk of cross-contamination, offering high quality, low volume dispensing for a wide range of applications.

Do you want to see how digital dispensing works in practice? Schedule an online software demo!

Tab 01 / Overview

Are you tired of manual pipetting?

Presenting the way to make serial dilutions obsolete. Set up your assay by dispensing directly from the stock solution – now you can use different plate formats up to 1536 wells! Take advantage of our dedicated software wizards for dose-response, synergy, PCR or enzyme profile experiments.

Finally - normalize and randomize the layout to improve reliability of collected data. Find out how Tecan D300e can save your day in the lab!

The Tecan D300e Digital Dispenser offers a simple method for setting up PCR reactions, generating enzyme profiles, synergy experiments and dose-response curves.

Using HP’s Direct Digital Dispensing technology, it provides picoliter to microliter non-contact dispensing of liquids directly into the assay plate, saving time, minimizing consumption of valuable samples and accelerating research.

Dispensehead cassettes

Tecan dispensehead casettes
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D300e highlights

Flexible Plate Maps

Create any plate map and dispense any dose in any well. Even randomize the plate with only one click! The Tecan D300e's software is so simple to use that one can create any plate map desired in only minutes!

Digital Titration

Use your time more efficiently in follow up studies. D300eControl software makes it easy to generate dilution series, leading to more precise determination of Hill slopes and IC50 values.

Minimizes manual pipetting, enabling scientists to focus on more important tasks and reducing repetitive strain injuries. Enhances throughput with simple, yet sophisticated, software and application-focused wizards. Automatically calculates all concentrations and volumes for faster, simpler plate set-up.

Dispense your reagents directly into your assay plate and onto your cells.

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qPCR Set-Up

The Tecan D300e’s PCR wizard makes it quick and easy to set up (q)PCR reactions in just 4 guided steps. Miniaturize your reaction volume and save on reagents and precious samples.

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Enzyme Profiles

The Tecan D300e’s Enzyme Profile wizard makes it quick and easy to set up titrations using differing combinations of enzymes or substrates in aqueous buffers, and inhibitors or other small molecules in DMSO.

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Drug Combination Studies

In vitro drug combination studies are becoming more and more. The Tecan D300e, due to independent dosing of each compound and a software wizard, finally makes it possible to execute these experiments with ease.

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Time Savings

The Tecan D300e makes long, laborious workflows possible to standardize. This technology removes the need for many wasteful steps, getting to results faster.

The Tecan D300e is a cost-effective solution designed to accelerate drug discovery workflows by:

  • Reducing titration times from hours or days to minutes
  • Allowing drug discovery biologists to quickly and easily perform their own titrations
  • Providing greater access to titration and IC50 data
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Tecan Journal 03/2015

Tecan’s D300e offers fast liquid transfer down to picoliter levels

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Tecan Journal 01/2015

HP D300 now offers aqueous liquid dispensing

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Tab 02 / Software

The system’s powerful software combines advanced experimental design with intuitive operation, allowing you to set up a wide range of experiments – including synergistic  studies and randomized plate layouts – in minutes, saving you time without compromising on quality.

D300e Control Software

Tecan D300e can deliver any dose in any well

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Plate randomization can be made with one click

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Set up your PCR reaction in just 4 simple steps

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Synergy Wizard allows users to create synergy experiments in minutes

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Tab 03 / Videos


Get an introduction to the D300e and its various functions and software wizards

Find out how easy it is to operate the D300e digital dispenser

Eliminate the need for serial dilutions

Synergy experiments made easy

Normalize and randomize assays

Enzyme Profile Wizard

PCR Wizard

Watch a webinar to learn about the benefits of drug-drug combination studies

Join this webinar to get more information about synergy experiments.

Tab 04 / Literature


Repetitive manual pipetting and tedious serial dilutions for drug discovery assays just became a thing of the past.

With Tecan’s D300e Digital Dispenser, you can automatically dispense any volume into any well of any assay plate, quickly and easily. Simply add your liquid to the Dispensehead and let the instrument do the rest.


T8+ Dispensehead Cassette

D4+ Dispensehead Cassette

Application Notes

The data presented in this application note demonstrates that the D300e can be used to miniaturize qPCR reactions to volumes as low as 2 μl and in addition offers greater process security and speed of the automated set-up.

The application note shows that the D300e is a suitable tool for qPCR set-up, offers significant sample, primer and reagent savings, and greatly reduces workflow time.

The data presented in this application note underlines the suitability of the D300e for protein-based assays.

The results generated with the D300 are consistent with an expertly performed manual process, but with the benefit of significant time savings and the elimination of serial dilution.

Technical Notes

This technical note demonstrates, how easy it is to generate even the most complex plate layouts, like enzyme profiles, with the D300.

Tecan Journal Articles

The direct digital dispensing capabilities of the HP D300 are helping to identify potential new treatments for acute lymphoblastic leukemia at University Children’s Hospital Zurich. By enabling picoliter drug volumes to be dispensed straight into cell-based assay plates, the system is helping researchers to rapidly test novel drug combinations directly in patient-derived leukemia cells.

The HP D300 Digital Dispenser has enabled Translational Drug Development in the USA to automate its workflows, increasing sample throughput and the number of cell line panels handled, as well as enhancing workflow efficiency.

AstraZeneca scientists engaged in surface plasmon resonance studies are reaping the benefits of a recently acquired HP D300 Digital Dispenser, saving time, enhancing consistency and using significantly less compound for dose-response experiments.

Tecan has worked with promega to demonstrate the suitability of the Infinite® 200 PRO and HP D300 Digital Dispenser for real-time kinetic investigation of cytotoxicity using Promega’s CellTox™ Green Dye.

GlaxoSmithKline’s quest for improved anti-malarial treatments has been revolutionized by the speed and reliability of the HP D300 Digital Dispenser. This innovative system has increased throughput for standard drug titrations and enabled combinations of candidate compounds to be tested much more easily.

More than skin deep
[TJ 01/2013]

Symrise is using an HP D300 Digital Dispenser to improve throughput and reproducibility in its cell and molecular biology laboratories, offering more reproducible results for cosmetic active ingredient testing.

The Stanford genome Technology Center has invested in an HP D300 Digital Dispenser to study the effects of small molecule chemical inhibitors in yeast, enabling more experiments to be carried out more easily, and in less time.

TES Pharma is a specialist biotechnology company which performs molecular, cellular and ADME screening for drug discovery. The Company has recently purchased an HP D300 Digital Dispenser, and is now reaping the benefits of direct titration with improved assay reproducibility and more time to perform exploratory research.

In the last issue of the Tecan Journal, we announced the availability of the revolutionary HP D300 Digital Dispenser exclusively through Tecan’s Life Science Business. As customers in Europe and North America begin to place their orders, Kevin Peters, HP Global Sales Development Manager, reports on early customer experiences with the new technology.

Related Products
D4+ Dispensehead cassettes

For high volume non contact dispensing from 1nL to 10µL 20 cassettes/sales unit

T8+ Dispensehead cassettes

For non contact dispensing from 11pL to 10µL 20 cassettes/sales unit

Trustmarks & Awards
For research use only. Not for use in diagnostic procedures.
Tecan D300e Digital Dispenser is a product of HP Inc. CA, USA.