What methods do you recommend for RNA isolation?
We recommend a column-based method, including:
- Norgen Biotek Total RNA Purification Kit
- Zymo Research Quick-RNA™ Kits
- Arcturus PicoPure® RNA Isolation Kit
- Ambion PureLink® RNA Mini Kit
- Qiagen RNeasy Kits
Organic methods such as TRIzol® Reagent should be subsequently followed with a column-based clean-up method.
Can I use TRIzol® or other phenol-chloroform based extractions for RNA isolation?
We do not recommend the use of TRIzol or similar methods as any carryover of organic solvents may inhibit downstream enzyme activity. If using TRIzol extracted RNA, we recommend using a column-based purification of the RNA prior to input into the kit.
Can I use carrier RNA during RNA isolation?
We do not recommend the use of carriers during RNA isolation. If a carrier is required, please contact Tecan NGS Technical Support for more information.
Can I use Universal Plus mRNA-Seq with RNA from any organism?
Universal Plus mRNA-Seq has been designed for use with total RNA inputs containing polyadenylated transcripts. Universal Plus mRNA-Seq is compatible with polyadenylated RNA from any organism.
Do I need to use high-quality total RNA?
Yes, high-quality RNA is required for efficient poly(A) selection.
Can contaminating genomic DNA interfere with Universal Plus mRNA Seq?
Contaminating genomic DNA can be incorporated into libraries and inclusion of a DNase treatment during RNA isolation is recommended.