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MagicPrep NGS system is a new category of automated laboratory equipment that provides push button simplicity for NGS library preparation for Illumina sequencing platforms.
The MagicPrep NGS system provides a complete solution that includes the instrument, software, pre-optimized scripts, reagents and consumables.
The MagicPrep NGS system integrates everything you need for library preparation; all you need to do is add your samples.
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Tab 01 / Overview
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With the MagicPrep NGS system, all reagents and consumables are provided pre-aliquoted in a single kit. The only thing you need to provide are your samples. No need to make master mixes or buffers. No need to purchase other reagents. Simply insert the cartridges into the instrument, start the run, and continue your day.
Tecan’s new MagicPrep NGS system achieves greater than 99% mean success rate*. This system utilizes a novel reagent dispensing technology along with proven technology to provide a reliable and reproducible solution while minimizing the possibility of user errors. MagicPrep NGS provides a fully automated and reproducible workflow, allowing labs to focus on their next breakthroughs and not on creating libraries.
The MagicPrep NGS system provides true walk-away automation with no additional user interaction after starting the run. Once the run has been started, you can focus on your experiments and let MagicPrep NGS take care of the library preparation. The MagicPrep NGS instrument can store the libraries without evaporation for up to 65 hours. Start your run at any time and then retrieve your libraries when you are ready.
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Tab 02 / DNA SEQ
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The Revelo DNA-Seq Mech for MagicPrep NGS library preparation kit provides all the reagents for generating DNA-Seq libraries on the MagicPrep NGS system. This kit features a broad input range from 50 ng to 500 ng of prefragmented genomic DNA and UDI adaptors for flexible multiplexing. This kit together with the MagicPrep NGS system provides a fully automated solution for DNA-Seq library preparation with a 10 minute setup time.
The Revelo DNA-Seq Mech for MagicPrep NGS kit was used to generate libraries from 100 ng of a pre-fragmented mix of three bacterial genomes with varying GC content (S. aureus (32% GC); E. coli (50% GC) and R. sphaeroides (68% GC)). The fraction of reads from each genome is similar to results using a manual workflow demonstrating a low GC bias during automated library construction.
Specification |
Specification/Description |
|---|---|
Input type |
Input type DNA |
Input amount: |
Input amount: 50 ng - 500 ng |
# Reactions available: |
# Reactions available: 32 |
Sample Indexes available |
Sample Indexes available Up to 96 UDI |
Sequencing platforms |
Sequencing platforms Illumina |
The Revelo DNA-Seq Enz for MagicPrep NGS library preparation kit provides all the reagents for generating DNA-Seq libraries on the MagicPrep NGS system. This kit features a broad input range from 50 ng to 500 ng of intact genomic DNA, robust enzymatic DNA fragmentation and UDI adaptors for flexible multiplexing. This kit together with the MagicPrep NGS system provides a fully automated solution for DNA-Seq library preparation with a 10 minute setup time.
Libraries were generated with 100 ng of a three bacterial genome mix with varying GC content (S. aureus (32% GC); E. coli (50% GC) and R. sphaeroides (68% GC)) using the Revelo DNA-Seq Enz for MagicPrep NGS kit. All genomes were represented as expected in the sequencing data. Insert size analysis shows similar insert sizes for all three genomes indicating that DNA fragmentation was not biased by the GC content.
Specification |
Specification/Description |
|---|---|
Input type |
Input type DNA |
Input amount: |
Input amount: 50 ng - 500 ng |
# Reactions available: |
# Reactions available: 32 |
Sample Indexes available |
Sample Indexes available Up to 96 UDI |
Sequencing platforms |
Sequencing platforms Illumina |
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Tab 03 / mRNA SEQ
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The Revelo mRNA-Seq for MagicPrep NGS library preparation kit provides all the reagents for generating stranded mRNA-Seq libraries on the MagicPrep NGS system. This kit features a broad input range from 10 ng to 1000 ng of total RNA and UDI adaptors for flexible multiplexing. This kit together with the MagicPrep NGS system provides a fully automated solution for mRNA-Seq library preparation with a 10 minute setup time.
Libraries were generated starting with 10 (16 libraries), 100 (24 libraries) or 1000 ng (16 libraries) of K562 total RNA. Data analysis showed similar general library metrics and consistent gene detection across the 100-fold difference in input.
Specification |
Specification/Description |
|---|---|
Input type |
Input type Total RNA |
Input amount: |
Input amount: 10 ng - 1 μg |
# Reactions available: |
# Reactions available: 32 |
Sample Indexes available |
Sample Indexes available Up to 96 UDI |
Sequencing platforms |
Sequencing platforms Illumina NGS |
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Tab 04 / Reliability
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MagicPrep NGS system is designed to provide a simple, reliable solution for automated library preparation. The MagicPrep NGS system was extensively tested to optimize the reagent formulation, automation script and consumables to minimize library failures.
MagicPrep NGS library construction success rate was tested using control samples using recommended input amounts and PCR cycles. Libraries were considered successful if the total library yield was greater or equal to 200 ng. Expected library size distribution was confirmed for all libraries using the Fragment Analyzer.
Library quality and success is impacted by the sample quality, the input amount and potential inhibitors in the sample. Samples with lower quality may see variable success rates.
The MagicPrep NGS system was used to generate libraries for Illumina sequencing using control samples A total of 59 runs (472 samples), a combination of both mRNA-Seq and DNA-Seq runs, were performed on over 10 different instruments. The mean library success rate was calculated for all runs regardless of application. Overall, the mean success rate for the MagicPrep NGS system is greater than 99%.
The MagicPrep NGS system provides consistent library construction within runs and between multiple runs. Forty libraries (5 runs), starting with 100 ng of K562 total RNA, were generated with the Revelo mRNA-Seq for MagicPrep NGS kit on a single instrument. The total yield of each library is graphed to demonstrate the consistency of library yields. Each run has a CV of between 5.8%-16.8%, indicating that the eight samples within each run have a consistent yield. Additionally, the library yields generated among all 5 runs are similar, demonstrating the consistency of the MagicPrep NGS system.
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Tab 05 / faq
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The MagicPrep NGS system is 58 cm x 25 cm x 31 cm (L x W x H). The instrument weights 18.8 kg.
The MagicPrep NGS system operating conditions for NGS library preparation are:
The MagicPrep NGS system should be placed in a location that provides sufficient space from walls or other objects that might limit airflow. It is recommended to have an unobstructed gap of at least 15 cm (6 inches) to the sides and the rear of the instrument.
The MagicPrep NGS instrument requires the following power supply: 100-240 VAC, 50-60 Hz.
The MagicPrep NGS system comes with the instrument, power cord, sample deck and sample deck stand.
No, the MagicPrep NGS system is only compatible with Tecan reagents./p>
It is possible to perform a MagicPrep NGS run with less then 8 samples. The consumables, however, are one-time use only. The unused reagents can not be recovered or used for a subsequent run.
Each kit is sufficient for 4 runs (32 samples). Each kit comes with 4 reagent cartridges, magnetic beads, and 4 sets of sample deck consumables.
Perform a power cycle and restart the system. To perform a power cycle, turn off the MagicPrep NGS system and wait for the LED strip to turn off completely. Once the system is completely off, power on the MagicPrep NGS system. If the error persists, please contact Tecan Customer Support.
Perform a power cycle to reinitialize the system. Make sure that the LED strip is completely off before restarting the system. If the error persists, please contact Tecan Customer Support.
The MagicPrep NGS system will not work with previously used consumables. Make sure that new consumables are being used. Wipe off any moisture that could prevent the barcodes from being read and reload the instrument. If the error persists, please contact Tecan Customer Support.
The MagicPrep NGS system will not work with previously used consumables. Make sure that new consumables are being used. Wipe off any moisture that could prevent the barcodes from being read and reload the instrument. If the error persists, please contact Tecan Customer Support.
Revelo DNA-Seq Mech for MagicPrep NGS consists of Sample Deck components, Reagent Cartridges and a single tube of Agencourt beads.
A comprehensive list of required and recommended equipment can be found below:
Equipment
Reagents
Supplies and Labware
We recommend a column-based extraction method, including: Qiagen QIAprep Miniprep or DNeasy MiniPrep kits, Zymo Quick-DNA kits and Thermo Fisher PureLink Genomic DNA kits.
We do not recommend the use of these methods as any carry-over of organic solvents may inhibit downstream enzyme activity. If phenol-chloroform reagents are used, we recommend using a column-based purification of the DNA prior to input into the kit.
Revelo DNA-Seq Mech for MagicPrep NGS can be used with 50 ng to 500 ng purified, high-quality DNA. Input amounts outside this range may affect reaction stoichiometry, resulting in sub-optimal libraries that produce variable and unsatisfactory results. Use of lower inputs or degraded samples will potentially produce insufficient yields depending on the requirements of the analytical platform.
Revelo DNA-Seq Mech for MagicPrep NGS has been tested with DNA inputs isolated from human and bacterial samples. However, any DNA sample should yield a sequenceable library. For more information, contact Tecan NGS Technical Support at techserv-gn@tecan.com.
Yes, the input DNA must be pre-fragmented prior to being used with this kit.
We have evaluated only Covaris fragmented DNA during the development of MagicPrep NGS DNA-Seq. Other mechanical means of fragmentation, such as sonication, may be suitable. We also offer Revelo DNA-Seq Enz for MagicPrep NGS which features enzymatic fragmentation of high molecular weight genomic DNA.
Tecan does not provide the reagents used in the fragmentation steps. We suggest the Covaris instrument be utilized for DNA fragmentation.
Revelo DNA-Seq Mech for MagicPrep NGS performs 8 preps with every run. Empty positions will not produce libraries. Reagent cartridges and sample deck components cannot be reused.
Yes, the user will have the opportunity to adjust cycle number from the recommended value selected based upon sample input. Table 2 of the User Guide contains guidelines on the number of cycles to use, though some samples may perform better with a different number of cycles.
Yes, although we do not recommend the use of expired reagents, the user will still have the option to run the protocol after the instrument detects the use of expired reagents.
The adaptors add 133 bp to the library.
Revelo DNA-Seq Mech for MagicPrep NGS libraries will exhibit a size range proportional to the input of fragmented DNA provided by the user. For input DNA fragmented to 300–350 bp, total library size will be 400–500 bp.
Revelo DNA-Seq Mech for MagicPrep NGS libraries are compatible with Illumina sequencing platforms utilizing standard on-board sequencing primers.
Please follow manufacturer’s recommendations for library QC, quantification, color-balancing and loading of the amplified library on the sequencer.
Each index is a minimum edit distance of three from any other index. This allows error correction of one mismatch during demultiplexing. For further details on the index design strategy, please refer to Faircloth BC, Glenn TC (2012), Not All Sequence Tags Are Created Equal: Designing and Validating Sequence Identification Tags Robust to Indels. PLoS ONE 7(8): e42543. doi:10.1371/journal.pone.0042543.
Revelo DNA-Seq Enz for MagicPrep NGS consists of sample deck components, reagent cartridges and a single tube of Agencourt beads.
A comprehensive list of required and recommended equipment can be found below:
Equipment
Reagents
Supplies and Labware
We recommend a column-based extraction method, including: Qiagen QIAprep Miniprep or DNeasy MiniPrep kits, Zymo Quick-DNA kits and Thermo Fisher PureLink Genomic DNA kits.
We do not recommend the use of these methods as any carry-over of organic solvents may inhibit downstream enzyme activity. If phenol-chloroform reagents are used, we recommend using a column-based purification of the DNA prior to input into the kit.
Revelo DNA-Seq Enz for MagicPrep NGS can be used with 50 ng to 500 ng purified, high-quality DNA. Input amounts outside this range may affect reaction stoichiometry, resulting in sub-optimal libraries that produce variable and unsatisfactory results. Use of lower inputs or degraded samples will potentially produce insufficient yields depending on the requirements of the analytical platform.
Revelo DNA-Seq Enz for MagicPrep NGS has been tested with DNA inputs isolated from human and bacterial samples. However, any DNA sample should yield a sequenceable library. For more information, contact Tecan NGS Technical Support at techserv-gn@tecan.com.
The MagicPrep NGS system performs 8 preps with every run. Empty positions will not produce libraries. Reagent Cartridges and Sample Deck components cannot be reused.
Yes, the user will have the opportunity to adjust cycle number from the recommended value selected based upon sample input. Table 2 of the User Guide contains guidelines on the number of cycles to use, though some samples may perform better with a different number of cycles.
Yes, although we do not recommend the use of expired reagents, the user will still have the option to run the protocol after the instrument detects the use of expired reagents.
The adaptors add 133 bp to the library.
Revelo DNA-Seq Enz for MagicPrep NGS libraries generated with high-quality human DNA contain inserts that are between 300-350 bp on average. Total library size will be 400–500 bp.
Revelo DNA-Seq Enz for MagicPrep NGS libraries are compatible with Illumina sequencing platforms utilizing standard on-board sequencing primers.
Please follow manufacturer’s recommendations for library QC, quantification, color-balancing and loading of the amplified library on the sequencer.
Each index is a minimum edit distance of three from any other index. This allows error correction of one mismatch during demultiplexing. For further details on the index design strategy, please refer to Faircloth BC, Glenn TC (2012), Not All Sequence Tags Are Created Equal: Designing and Validating Sequence Identification Tags Robust to Indels. PLoS ONE 7(8): e42543. doi:10.1371/journal.pone.0042543.
Revelo mRNA-Seq for MagicPrep NGS consists of Sample Deck components, Reagent Cartridges and a single tube of Agencourt beads.
A comprehensive list of required and recommended equipment can be found below:
Equipment
Reagents
Supplies and Labware
We recommend a column-based method, including:
Organic methods such as TRIzol® Reagent should be subsequently followed with a column-based clean-up method.
We do not recommend the use of TRIzol® or similar methods as any carry-over of organic solvent may inhibit downstream enzyme activity. If using TRIzol extracted RNA, we recommend using a column-based purification of the RNA prior to input into the kit.
No. Residual carrier RNA can result in poor data quality.
Revelo mRNA-Seq for MagicPrep NGS can be used with 10 ng to 1 μg purified, high-quality (RIN ≥7.0) total RNA. Input amounts outside this range may affect reaction stoichiometry, resulting in sub-optimal libraries that produce variable and unsatisfactory results. Lower input amounts will potentially result in insufficient yields depending on the requirements of the analytical platform.
No rRNA depletion or poly(A) enrichment step is needed. Use total RNA inputs with Revelo mRNA-Seq for MagicPrep NGS.
Yes. When using purified total RNA samples, large amounts of contaminating genomic DNA may amplify during the process. For this reason DNase treatment is recommended prior to running Revelo mRNA-Seq for MagicPrep NGS.
Revelo mRNA-Seq for MagicPrep NGS has been designed and tested only with total RNA inputs isolated from human samples. However, any input RNA sample containing some fraction of 3ˇ-poly(A) sequence should yield a sequenceable library. For more information, contact Tecan NGS Technical Support at techserv-gn@tecan.com.
Revelo mRNA-Seq for MagicPrep NGS performs 8 preps with every run. Empty positions will not produce libraries. Reagent Cartridges and Sample Deck components cannot be reused.
No. Revelo mRNA-Seq for MagicPrep NGS utilizes poly(A) selection to target poly-adenylated mRNA transcripts. This yields sequencing data with minimal rRNA contamination.
Yes, the user will have the opportunity to adjust cycle number from the recommended value selected based upon sample input. Table 2 of the User Guide contains guidelines on the number of cycles to use, though some samples may perform better with a different number of cycles.
Yes, libraries prepared with Revelo mRNA-Seq for MagicPrep NGS are stranded. The first sequencing read represents the sense strand.
Yes, although we do not recommend the use of expired reagents, the user will still have the option to run the protocol after the instrument detects the use of expired reagents.
The adaptors add 133 bp to the library.
Revelo mRNA-Seq for MagicPrep NGS libraries generated with high-quality human total RNA contain inserts that are between 300–350 bp on average. Total library size will be 400–500 bp.
Revelo mRNA-Seq for MagicPrep NGS libraries are compatible with Illumina sequencing platforms utilizing standard on-board sequencing primers.
Please follow manufacturer’s recommendations for library QC, quantification, color-balancing and loading of the amplified library on the sequencer.
Each index is a minimum edit distance of three from any other index. This allows error correction of one mismatch during demultiplexing. For further details on the index design strategy, please refer to Faircloth BC, Glenn TC (2012), Not All Sequence Tags Are Created Equal: Designing and Validating Sequence Identification Tags Robust to Indels. PLoS ONE 7(8): e42543. doi:10.1371/journal.pone.0042543.
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Tab 06 / Literature
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Tecan’s MagicPrep NGS is a new type of automation solution for NGS library preparation that just works. Reimagined and redesigned, it offers a simple solution that anyone can use. Tecan’s MagicPrep NSG system comes with numerous benefits and features
*Success rate is based on mean internal data. Sample quality and variability may have an impact on the success rate.
For research use only. Not for use in diagnostic procedures.