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Featured Publications

Summaries of selected peer-reviewed publications. These reviews highlight some of the ways Tecan's NGS technologies are furthering scientific research.

Sequencing identifies multiple, early introductions of SARS-CoV2 to New York City Region

April 21, 2020

Maurano MT, Ramaswami S, Westby G, Zappile P, Dimartino D, Shen G, Feng X, Ribeiro-dos-Santos AM, Vulpescu NA, Black M, Hogan MS, Marier C, Meyn P, Zhang Y, Cadley J, Ordoñez R, Luther R, Huang E, Guzman E, Serrano A, Belovarac B, Gindin T, Lytle A, Pinnell J, Vougiouklakis T, Boytard L, Chen J, Lin LH, Rapkiewicz A, Raabe V, Samanovic-Golden MI, Jour G, Osman I, AgueroRosenfeld M, Mulligan MJ, Cotzia P, Snuderl M, Heguy A

SARS-CoV-2 is being studied on a global scale to understand its evolution, spread and impact on human health. Maurano et al. from New York University School of Medicine successfully performed large scale genomic surveillance in the NYC metropolitan area from 236 patient samples (Nasopharyngeal swabs). The researchers at NYU chose Trio RNA-Seq for its robust performance with degraded and low viral titer samples. The results from this publication indicated that there was undetected community spread and concluded that the major strains for the outbreak originated in Europe.

doi: 10.1101/2020.04.15.20064931

Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development

9 November, 2015

Zylicz JJ, Dietmann S, Günesdogan U, Hackett JA, Cougot D, Lee C, Surani MA

Development is very dynamic process that is intimately regulated by changes in chromatin modifications. In this study, Zylicz and colleagues investigated how epigenetics influences the genome during early embryonic development. Zylicz, et al. performed a comprehensive analysis utilizing Ovation RNA-Seq System V2, Ovation Rapid Library System, Ovation Ultralow Library System, and Ovation Ultralow Methyl-Seq Library System to understand how epigenetics influences transcriptional regulation and chromosomal modifications. Their findings identify two enzymes (EZH2 and G9a) that dramatically affect histone methylation and thus impact cell fate decisions during development.

Pages:e09571 doi:10.7554/eLife.09571

Applying the INTACT method to purify endosperm nuclei and to generate parental-specific epigenome profiles

5 January, 2017

Moreno-Romero J, Santos-González J, Hennig L, and Köhler C

This study features a novel method for successfully isolating nuclei from Arabidopsis to investigate the epigenetic landscape of two parental accessions. Learn how the authors use Ovation Ultralow Library System and Ovation Ultralow Methyl-Seq Library System to study histone modifications via ChIP-Seq and DNA methylation patterns via Methyl-Seq, respectively. In the publication, the authors meticulously detail the protocol that they used to achieve cell-type-specific nuclei for the epigenome study.

Pages: 238-254 doi: 10.1038/nprot.2016.167

TT-seqmaps the human transient transcriptome

3 June, 2016

Schwalb B, Michel M, Zacher B, Frühauf K, Demel C, Tresch A, Gagneur J, Cramer P

This study describes a new technique transient transcriptome sequencing (TT-Seq) to map transcriptionally active regions and enable estimation of RNA synthesis and degradation rates. Learn how the authors use the Ovation Universal RNA-Seq System to construct dimer-free libraries from low input fragmented RNA. The whole transcriptome approach with integrated ribosomal depletion allowed for study of non-coding RNAs that are generally rapidly degraded and have been difficult to detect and study.

Pages: 1225-8 doi: 10.1126/science.aad9841