Tecan’s Unique Dual Indexes allow you to uniquely label up to 384 samples with a P7 and a P5 barcode, which are attached to the library fragments in a simple ligation step.
Ligation of two unique barcodes to each of the library fragments (one at each end) mean that the fragments are identified twice. This dual labeling is key to the detection of index hopping, a sequencing phenomenon that occurs at a low level with Illumina sequencing instruments and is most prevalent on patterned flow cells like those used by the Illumina NovaSeq.
If left unchecked index hopping can lead to mis-assignment of reads to the incorrect sample.
Using Tecan’s Unique Dual Indexes in library prep kits, enable the detection and elimination of mis-assigned reads from the sequence dataset.