Support Center

Product selection guide

Find the right product for your experiment.

For further questions regarding our kits contact Tecan's NGS scientific support.




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Tab 01 / DNA-Seq
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DNA-Seq solutions

Input Amount   10ng – 500ng 10ng – 500ng 200ng – 500ng 10pg – 100ng 10ng – 100ng
Input Type  
High quality
gDNA
High quality
gDNA
High quality
gDNA
High Quality
gDNA, FFPE,
cfDNA or
degraded DNA
High quality
gDNA
Enzymatic Fragmentation   no yes yes no yes
PCR-Free   no no yes no no
Unique Dual Index   yes yes yes yes no
High-throughput Multiplexing   yes yes no no yes
Molecular Tags   no no no yes no
Custom Target Enrichment Probes   no no no no yes
NuQuant®   yes yes no no no
    Celero™
DNA-Seq
Celero™ EZ
DNA-Seq
Rapid EZ
DNA-Seq™
Ovation
Ultralow
DNA-Seq V2
Allegro®
Targeted
Genotyping V2

DNA-Seq_Selection_Guide-min




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Tab 02 / RNA-Seq
(Standard Input)
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RNA-Seq solutions

Standard input library preparation solutions for Illumina sequencing.

Input Amount 10ng - 1µg 10ng - 500ng 100ng - 500ng 10ng - 250ng
Input Type
High quality
total RNA
High quality,
FFPE or
degraded
total RNA
High quality
or degraded
bacterial
RNA
High quality,
FFPE or
degraded
total RNA
Chemical Fragmentation yes yes no no
Strand Specific yes yes yes yes
Targeted Depletion yes yes yes yes
Molecular Tags yes yes yes yes
Up to 192 Unique Dual Indexes yes yes yes yes
NuQuant® yes yes no yes
Platform Compatibility Illumina Illumina Illumina Illumina
  Universal Plus
mRNA-Seq with
NuQuant
Universal Plus
Total RNA-Seq
with NuQuant
Universal
Prokaryotic
RNA-Seq
Universal
RNA-Seq
with NuQuant

Standard input library preparation solutions for Illumina sequencing.




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Tab 03 / RNA-Seq
(Ultra-Low Input)
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RNA-Seq solutions

Ultra-low input library preparation solutions for Illumina sequencing.

Input Amount 10pg - 10ng 500pg - 50ng 500pg - 100ng 250pg - 10ng
Input Type
High
quality,
FFPE, cf RNA
or degraded
total RNA
High
quality,
FFPE or
degraded
total RNA
High
quality,
FFPE or
degraded
total RNA
High
quality,
nasal swabs
or degraded
total RNA
Isothermal Amplification no yes yes yes
Strand Specific yes no no no
Targeted Depletion yes yes no yes*
Molecular Tags yes no no no
Up to 96 Unique Dual Indexes no yes no yes
NuQuant® no no no yes
Platform Compatibility Illumina Illumina Illumina
Ion
Illumina
  Ovation®
SoLo® RNA-Seq
System
Trio
RNA-Seq
Ovation
RNA-Seq
System V2
Revelo™
RNA-Seq
Separate Library Prep Kit Required     Ovation
Ultralow
System V2
*human
rRNA only

Ultra-low input library preparation solutions for Illumina sequencing. style=




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Tab 04 / Methyl-Seq
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Methyl-Seq solutions

Input Amount 10ng – 300ng 100ng
Input Type
High quality,
FFPE, cell-free
or degraded
DNA
High quality
genomic DNA
Enzymatic Fragmentation no yes
Directional Libraries yes yes
Diversity Adaptors no yes
Molecular Tag no yes
Oxidative Bisulfite Reagents Included yes yes
Up to 96 barcodes yes yes
Automation-ready yes yes
Platform Compatibility Illumina Illumina
  Ultralow
Methyl-Seq™
with
TrueMethyl®
oxBS
Ovation® RRBS
Methyl-Seq
with
TrueMethyl®
oxBS

PSG_04_Methyl-Seq_solutions

 




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Tab 05 / Microarrays and qPCR
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RNA sample preparation

 

Starting materials and sample sizes

Platform

Good Quality
>50 ng

Good Quality
5–50 ng

Good Quality &
Degraded
500 pg–50 ng

Whole Blood
5–50 ng

FFPE
50 ng

Affymetrix GeneChip®
3’ Arrays

Ovation® RNA Amplification System

V2 Encore Biotin Module

Ovation RNA Amp System V2

Encore Biotin Module

Ovation Pico WTA System V2

Encore Biotin Module

Ovation RNA Amp System V2

Ovation WB Reagent

Encore Biotin Module

Ovation FFPE WTA System

Encore Biotin Module

Affymetrix GeneChip
Exon/Gene ST Arrays

 

Ovation Pico WTA System V2

Ovation PicoSL WTA System V2

Encore Biotin Module

Ovation Pico WTA System V2

Ovation PicoSL WTA System V2

Encore Biotin Module

Ovation Pico WTA System V2

Ovation PicoSL WTA System V2

Encore Biotin Module

Ovation FFPE WTA System

Encore Biotin Module

Agilent Dual-Mode
Gene Expression Arrays

Ovation RNA Amplification System V2

Ovation RNA Amp System V2

Ovation PicoSL WTA System V2

Ovation RNA Amp System V2

Ovation FFPE WTA System*

Quantitative PCR

 

Crescendo cDNA Synthesis for qPCR

Crescendo cDNA Synthesis for qPCR

Crescendo cDNA Synthesis for qPCR

Crescendo cDNA Synthesis for qPCR

*May require optimization of fragment sizes

qPCR Product Matrix