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Tecan's NGS reagents offer innovative library preparation solutions for a broad range of sample types and inputs as low as 10 pg for DNA-Seq and RNA-Seq.
With unique technologies for rRNA depletion (AnyDeplete®), library quantification (NuQuant®), SNP genotyping (SPET®) and pathogen detection (SPIA®), Tecan offers library prep kits applicable for a variety of NGS applications.
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Tab 01 / DNA-Seq
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DNA-Seq is a technique used to determine information across the entire genome. This technique provides insight into the instructions for building an organism, understanding of genetic function or evolutionary changes.
Benefits of DNA-Seq?
DNA-Seq is useful to apply in many scientific areas including medical, agricultural, food, and biological sciences. DNA-Seq can be used for:
DNA-Seq library preparation solutions provide kits combining library preparation, library quantification NuQuant, and DimerFree® adaptor ligation in an integrated workflow.
Tecan's DNA-Seq library preparation kits are used globally by researchers in different scientific fields, with multiple peer-reviewed publications.
Innovative solution to streamline whole genome sequencing library preparation and quantification
Read moreTecan's DNA sequencing portfolio features a host of proprietary workflow enhancements that simplify the process, reduce time, and improve the quality of the DNA sequencing data. With innovative technologies leading to faster, more sensitive DNA sequencing, our DNA-Seq library prep kits enable various inputs and workflows that generate high-quality sequencing data regardless of the sample source.
Simple DNA-Seq library preparation kit that enables library construction in three steps. NuQuant library quantification technology included providing accurate library molarity in seconds.
DNA-Seq library prep kit with optimization free enzymatic fragmentation for a 3 step workflow Integrated with NuQuant library quantification.
Robust DNA-Seq library prep kit for a broad range of sample types starting with as little as 10 pg of input.
Simple and robust enzymatic PCR-free solution for DNA-Seq library preparation in less than 2.5 hours.
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Tab 02 / RNA-Seq
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RNA-Seq is a powerful technique that can examine the presence and quantity of RNA in samples by using next-generation sequencing (NGS).
This tool is able to provide insight into the transcriptome of the cell by using high-throughput sequencing methods. It can facilitate research in gene expression, novel genes (or transcripts) discovery, splicing, and mutation analysis.
Compared to gene expression microarrays and qPCR, RNA-Seq offers a broad range of advantages.
RNA-Seq is useful to apply in many scientific areas, including medical, agricultural, food, and biological sciences. RNA-Seq can be used for differential gene expression, variation detection, allele-specific expression, systems biology, and single-cell analysis.
Tecan's RNA-Seq library preparation kits are used globally by researchers in different scientific fields, with multiple peer-reviewed publications.
Maximize your sequencing results in blood samples by eliminating unwanted globin reads.
Read moreTecan's RNA-Seq library preparation kits are used globally by researchers in different scientific fields, with multiple peer-reviewed publications. The library preparation kits provide the first complete solution, combining library preparation, targeted transcript depletion (AnyDeplete®), library quantification (NuQuant®), and DimerFree adaptor ligation in a fully integrated workflow.
Use the RNA-Seq selection guide to find the right kit for your application
Revelo RNA-seq library preparation solutions combine proprietary technologies to provide a streamlined solution for whole transcriptome RNA-seq from low-input or poor quality samples for rare transcript deletion and unbiased pathogen discovery.
Streamlined solution for mRNA-Seq library preparation for sequencing on Illumina sequencers. This kit features a broad input range, UDI, and simple library quantification with NuQuant.
Streamlined solution for total RNA-Seq library preparation for sequencing on Illumina sequencers. This kit features broad input range, UDI adaptors, rRNA depletion with AnyDeplete and simple library quantification with NuQuant.
Unique RNA-Seq library prep combines three proprietary technologies to provide a streamlined solution for whole transcriptome RNA-Seq library preparation from low input and poor quality samples for rare transcript detection and unbiased pathogen discovery.
The Universal Prokaryotic RNA-Seq kit provides a streamlined solution for RNA-Seq library preparation for bacterial metatranscriptomic analysis with integrated rRNA depletion for a wide range of bacterial species.
The SoLo RNA-Seq System is the only whole transcriptome solution integrated with rRNA depletion for ultra-low input transcriptomic studies.
Single Primer Isothermal Amplification (SPIA) technology provides industry leading whole transcriptome reverse transcription and cDNA amplification for low input and degraded RNA samples. The amplified cDNA can be used in a range of applications from NGS library prep and qPCR to sample archiving.
Robust solution for stranded whole transcriptome RNA-Seq library preparation for any sample type and RNA quality, integrated target depletion after library construction and library quantification.
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Tab 03 / Targeted Genotyping
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Tecan’s Allegro® Targeted Genotyping provides a fast, scalable, cost-effective approach for targeted genotyping-by-sequencing on a wide variety of organisms using NGS.
This workflow combines three core technologies to enable efficient SNP interrogation:
Allegro offers high sample multiplexing capabilities, the ability to interrogate over 100,000 SNPs in a single assay, and flexible design possibilities for targeting new markers.
Allegro provides a fast, scalable, cost-effective approach to perform targeted genotyping-by-sequencing with the ability to interrogate over 100,000 SNPs in a single assay on a wide variety of organisms using next generation sequencing.
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Tab 04 / Methyl-Seq
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Tecan's Methyl-Seq product line combines library preparation and bisulfite sequencing conversion tools for a broad range of applications including NGS and methylation arrays. With the integration of TrueMethyl® oxBS-Seq chemistry, users can now gain new insights into epigenetic modifications with the ability to interrogate both 5mC and 5hmC in a single protocol. Streamlined, reliable, user-friendly protocols that deliver results.
Methyl-Seq products feature Tecan's DimerFree ligation technology to enable streamlined, simple workflows for a broad range of input quantities and sample types. The Ultralow Methyl-Seq library preparation kit provides a comprehensive approach to DNA methylation detection across the entire genome from many different sample types. The Ovation® RRBS Methyl-Seq kit enables a focused and cost-efficient approach to assessing methylation state of regions with high CpG density through a reduced-representation bisulphite sequencing (RRBS) method.
A fast and scalable solution for producing Reduced Representation Bisulfite Sequencing (RRBS) Methyl-Seq libraries with integrated diversity adaptors to eliminate the need for PhiX.
Unique TrueMethyl oxBS technology enables the most accurate 5mC identification for NGS, and the interrogation of 5hmC, a modified base that is not assayed by traditional bisulfite sequencing conversion methods.
Simple library preparation kit provides a fast and scalable solution for producing whole genome Methyl-Seq libraries from as little as 10 ng genomic DNA from a broad range of sample types and can be completed within 6 hours.
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Tab 05 / Microarrays and qPCR
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Tecan’s microarray products are considered the gold standard for gene expression studies using low and ultra low input samples.
The Ovation Pico WTA System V2 is a fast and simple method for preparing amplified cDNA from total RNA for gene expression analysis for whole transcript arrays.
A fast and simple method for preparing amplified cDNA from FFPE-derived total RNA.
Robust solution for enhanced qPCR detection from low input and degraded samples Quantitative PCR (qPCR) is a sensitive method that allows researchers to detect and quantify nucleic acids in a starting material.
The Ovation RNA Amplification System V2 provides a fast, simple and sensitive method for preparing amplified cDNA from total RNA for gene expression analysis for 3' arrays.
The Ovation Whole Blood Solution is comprised of a set of optimized kits and protocols to enable gene expression analysis of whole blood total RNA without the need for globin reduction procedures.
The Ovation PicoSL WTA System V2 is a cost-effective amplification system designed for high-throughput whole transcript array formats.
Provides a rapid, simple and easily automatable approach for fragmentation and labeling of amplified cDNA generated with Tecan amplification systems that is suitable for hybridization to Affymetrix GeneChip® arrays.
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Tab 06 / Automation
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Tecan Automation Solutions have been developed to streamline sample preparation technologies for NGS and other genomic assays in the Tecan portfolio.
DreamPrep NGS is Tecan's fully optimized solution for walkaway NGS library prep and QC on the Tecan Fluent® Automation Workstation. This system minimizes manual intervention, saving time and delivering consistent, sequencing-ready libraries for a wide range of research applications.
The simplicity and robustness of Tecan's protocols enable automation on a variety of platforms. Tecan's automation scientists work closely with each customer to produce automation control software scripts to meet individual needs. Please contact us to implement Tecan workflows on your favorite automation platform today.
| Platform | Fluent® | Freedom EVO® | PE Sciclone NGS and NGSx | Beckman Biomek FXp | Beckman i7 | Agilent Bravo |
| Universal Plus mRNA-Seq with NuQuant | ||||||
| Universal Plus Total RNA-Seq with NuQuant | ||||||
| Trio RNA-Seq | ||||||
| Ovation SoLo RNA-Seq | ||||||
| Ovation RNA-Seq System V2 | ||||||
| Universal RNA-Seq with NuQuant | ||||||
| Celero DNA-Seq | ||||||
| Rapid EZ DNA-Seq | ||||||
| Allegro Targeted Genotyping V2 | ||||||
| Ovation Ultralow System V2 | ||||||
| Ultralow Methyl-Seq | ||||||
| Ovation RRBS Methyl-Seq System | ||||||
| Revelo RNA-Seq library preparation kit |
Note: Our automation size kits are designed to process 96 reactions in a maximum of two batches of samples on the standard platforms listed. If more than two batches are processed from a single kit, there may be insufficient reagent for 96 reactions due to overages required in automation workflows.
For research use only. Not for use in diagnostic procedures.