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HydroFlex™ Plus microplate washer

Cells. ELISAs.

The HydroFlex Plus microplate washer is a truly flexible and upgradeable platform for automated microplate strip washing, magnetic bead washing and vacuum filtration in 96-well plate formats. It is an ideal solution for a wide range of cell-, bead- and ELISA-based applications, reflecting over 30 years of Tecan expertise in advanced liquid handling.
Overview
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Tab 01 / Overview
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Integrated application flexibility

Every HydroFlex Plus washer comes with microplate shaking and automated buffer switching included, ready for ELISA washing and gentle washing of cells. In addition, optional user-interchangeable plate carriers are available for easy switching between washing and filtration applications.

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HydroFlex Plus is equipped with a user-exchangeable plate carrier for:

  • Automated washing of ELISAs and gentle washing of adherent cells

Selection of of universal wash heads, supporting all application modules:

  • Eight-way manifold for parallel washing of eight wells
  • 16-way manifold for parallel washing of 16 wells

Process control option for reliability and safety:

The optional Liquid Level Detection (LLD) system controls the fill levels in the wash buffer and waste bottles, warning the user when a critical liquid level has been reached.

Personalize your HydroFlex plate washer:

Choose your favorite color:

  • White (standard)
  • Metallic red
  • Metallic blue

Ready for robotic integration:

Every HydroFlex plate washer is ready for integration onto the following automated platforms:

Highlights at a glance

  • Ready for automated washing of ELISAs, cells and protein arrays
  • Magnetic bead washing using powerful neodymium magnets**
  • Two magnets per well for efficient bead settling (patented design)**
  • Automated system for vacuum filtration using vacuum sensor**
  • Individual vacuum settings for each filtration step within a protocol**
  • Control of wash head positions and speed settings for gentle cell washing
  • Up to two aspiration points per well for minimal residual volumes
  • Simultaneous aspiration and dispensing for ELISA washing
  • Automated buffer switching and plate shaking capabilities
  • Controls up to four wash buffers
  • Ready for robotic integration
  • Tested reliability to minimize maintenance and service costs
  • IQ/OQ procedures available
  • Designed to meet the IVDR (EU) 2017/746 and 2011/65/EU RoHS (reduction of hazardous substances)
  • Supports FDA 21 CFR Part 11 functionality together with HydroControl™ software




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Tab 02 / Technology
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ELISA and cell washing*

Using adjustable wash settings and head positions for optimized results.

  • For gentle washing of adherent and weakly adherent cells
  • For efficient ELISA washing and minimum residual volumes

Liquid Level Detection (LLD)

The optional Liquid Level Detection (LLD) system controls the fill levels in the wash buffer and waste bottles, warning the user when a critical liquid level has been reached.

 

Eight- or 16-way manifold

Eight- or 16-way manifold for ELISAs and cellbased assays, allowing simultaneous washing of eight or 16 wells.




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Tab 03 / Software
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HydroControl™ software

The HydroFlex Plus can be fully programmed and operated as a standalone instrument using the built-in display and keypad, and features a selection of predefined cleaning protocols for easy maintenance.

Every HydroFlex Plus system is also supplied with Tecan's intuitive HydroControl software, providing example wash programs and a predefined plate library, as well as a simulation mode for offline operation. Wash programs created in HydroControl can be quickly transferred to the on-board memory of the HydroFlex Plus plate washer via a USB interface, using the download function.

 

Validated

  • The HydroFlex Plus plate washer has been designed to meet IVDR (EU) 2017/746 when configured for ELISA washing*
  • In combination with the HydroControl software, HydroFlex Plus provides 21 CFR Part 11 functionality, such as electronic records, electronic signatures, user administration and audit trail functions*




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Tab 04 / Literature
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Application Notes

Gentle washing of cultured cells in microplates

Using the Tecan HydroFlex Plus for gentle washing of A431- and weakly adherent P815-cell lines in 96-well plate format.

Automated washing of ELISpot assays

Using Tecan’s HydroFlex Plus microplate washer instead of typical manual wash procedures with a multichannel pipette.

Brochures

HydroFlex Plus – ELISA configuration

The reliable microplate washer for processing of ELISAs and cells in 96-well plates.

Specifications of the HydroFlex Microplate & Strip-Plate Washer

A list of all specification for the HydroFlex Microplate Washer.

Comparison of HydroFlex vs. HydroFlex Plus

A comparison of the main specifications of the HydroFlex & HydroFlex Plus.

Tecan Journal Articles

Automation of time-consuming washing and detection steps accelerates screening of protein-protein interactions
[TJ 3/2008]

The Protein Interaction Screening Unit at the German Cancer Research Center, Heidelberg, has automated the co-immune precipitation method for investigating protein-protein interactions (PPIs) using Tecan’s HydroFlex washer and Infinite® F200 microplate reader, increasing throughput up to 100-fold compared to manual methods, and greatly accelerating PPI screening.

Improved plate washing for ELISpot assays
[TJ 1/2011]

Researchers at the University of Modena and Reggio Emilia, Italy, have introduced automated washing for Enzyme-Linked ImmunoSpot (ELISpot) assays, using the HydroFlex microplate washer.




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Tab 05 / Specifications
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General specifications
     
Display unit   Liquid crystal display with two rows of 16 digits
Keyboard   Four-key membrane keyboard
Number of dispensing channels   1, 2 or 4 channels
Manifold types   8- or 16-way for parallel processing of 8- or 16-wells
Variable
     
Volume of solution dispensed   50-3,000 µl in 50 µl steps (washing)
50-400 µl in 50 µl steps (dispensing)
Dispensing accuracy   ≤1.2 % typical**
(300 µl dispense volume, dispense rate: 3, 8-way manifold)
Dispensing uniformity CV   ≤2.5 % over the plate typical**
(300 µl dispense volume, dispense rate: 3, 8-way manifold)
Residual volume   Crosswise aspiration: ≤1.8 μl per well typical** (96-well, flat-bottom Greiner® Bio-One plate)
(asp. rate: 3; asp. time: 4 sec; head speed: 10 mm/sec; 8-way manifold)
Interface   USB
Shaking   Linear shaking; high, medium and low settings
Power
     
Supply   Auto sensing for the settings
100-120 V or 220-240 V, 50/60 Hz
Consumption   65 VA
Physical
     
Outside dimensions for plate washing   Width: 27.5 cm; depth: 36.6 cm; height: 18.5 cm
Weight   6.6 kg
Color   White (standard), Metallic red, Metallic blue
** Specifications are subject to change. Typical performance values represent the average observed factory tested values. For product specifications refer to operators manual.

This web page may contain reference to applications and products which are not available in all markets. Please check with your local sales representative.

* The HydroFlex Plus has been designed to meet IVDR (EU) 2017/746 when configured for ELISA washing. In combination with the HydroControl software, it also provides FDA 21 CFR part 11 functionality.

** This optional functionality is available at extra cost for the general purpose article 30087531 Instrument HydroFlex for USA and Canada only.
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