Duo Digital Dispenser™

Picoliter dispensing redefined, now for single cells too. Meet Duo Digital Dispenser, the evolution of D300e.

Duo is the only device you need for your low-volume and single- to multi-cell dispensing workflows. This affordable benchtop instrument integrates all the advantages of proven microfluidic technology into one powerful solution.

Duo dispenses reagents and stock solutions with precision, from picoliters to microliters and single to multiple cells. Designed for dose-response curves, synergy experiments, miniaturized genomics workflows and single-cell analysis. Its intuitive software and adaptable design streamlines both high-throughput and low-volume workflows.

Do you want to see how digital dispensing works in practice? Schedule an online software demo!

Overview

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Streamline your workflow

Highest precision and speed

Dispense volumes from pico to microliters with picoliter precision. Isolate single cells in ~5 minutes across a 384-well plate, with >90 % isolation success and dispense reagents in <3 minutes.

Gentle on cells

The microfluidic technology ensures robust 90% cell viability and clonal outgrowth efficiency comparable to manual pipetting, without any risk of cross-contamination.¹

Accessible and flexible

Use the Duo Digital Dispenser the way you need it. Get your instrument at an affordable rate and purchase cassettes based on your specific dispensing needs - be it for single-cell dispensing, general liquid dispensing, or both.

Rely on proven technology

Powered by HP’s proven microfluidic digital dispensing technology and trusted by over 1’000 industry-leading customers. Expect consistent performance and a durable instrument built to last.

Truly plug-and-play

Ready within minutes after unboxing. Simply plug in your cable, install the highly user-friendly software, define your plate and dispensing pattern and hit run.

Featured Workflow

Combine cell and reagent dispensing on the same instrument.

Duo_KeyVisual_Process

Product features

Duo Digital Dispenser specifications

Specification	Specification/Cell dispensing	Specification/Reagent dispensing
Cell size range	Cell size range 7 – 25 µm	Cell size range -
Sample type	Sample type Wide variety of cell types²	Sample type Biomolecules, small molecules, nanoparticles
Qualified fluid classes	Qualified fluid classes Cell suspension	Qualified fluid classes DMSO, acetonitrile, aqueous solutions, proteomics solutions (such as trypsin, Iodoacetamide DTT, TCEP or TEAB), master mixes³
Formats to dispense on	Formats to dispense on 12 – 1536 well plates Customizable substrate, such as microscope slides Plate height 4-47mm
Dispensing technology	Dispensing technology Cell isolation based on impedance signal	Dispensing technology Reagent dispensing based on inkjet microfluidics technology
Dispensing speed	Dispensing speed 384-well plate in ~5 minutes	Dispensing speed 384-well plate in <3 minutes
Dispensing volume range	Dispensing volume range 480 pl - 20 µl	Dispensing volume range 7 pl - 200 µl (Depending on the cassette and fluid type)
Cell dispensing	Cell dispensing Dispense 1 to 25 cells per well	Cell dispensing -
Cell viability after dispensing	Cell viability after dispensing 90 % or greater¹	Cell viability after dispensing -
Software	Software Intuitive and easy to set-up protocols Automatically generates summary report, indicating successful single cell dispense and numbers of cells per well	Software Intuitive and easy to set-up protocols Automatically generates summary report, indicating volumes and concentrations
Overall instrument dimensions	Overall instrument dimensions 47 x 37 x 24 cm
Instrument weight	Instrument weight 16.1 kg

1) < 1 hour post-dispense

2) For a detailed list of cell types, please refer to the brochure.

3) This list includes common compatible fluids but is not exhaustive. For inquiries about additional fluids, please contact Tecan customer support.

Duo DispenseControl Software

The software behind Duo combines the trusted features of D300eControl and UnoControl to streamline your laboratory workflows with enhanced capabilities and efficiency. Experience the power of this advanced software and transform your experimental processes with ease.

Effortless experiment setup

Quickly configure a wide range of experiments, including cell isolation, synergistic studies and randomized plate layouts, in minutes.

Intuitive protocol guidance

Step-by-step assistance for single cell and/or reagent dispense protocols.

Immediate insights

Auto-generated summary reports provide detailed information on dispensed reagent volumes, utilized plates and cassettes, single-cell isolation success rates, and cell concentration metrics.

Enhanced workflow efficiency

Save time without compromising on quality, thanks to the software's user-friendly design and advanced features.

Streamline experiments with powerful DispenseControl Tools

Conduct titration, cell isolation or synergy experiments with ease using the powerful DispenseControl. Create precise, customized plate layouts and randomize them with a single click, optimizing your experimental design and efficiency.

Customize fluid classes for precise, reliable dispensing

Easily select the optimal fluid class for your experiments. Customize dispensing of DMSO, aqueous solutions or even cells to match your specific needs, ensuring precision and reliability across diverse applications.

Streamline experiments with powerful DispenseControl Tools

Step-by-step protocol guidance ensures smooth navigation through each phase of your experiment, while built-in features minimize human errors, enhancing efficiency and accuracy in your research process.

Automated reports offer key metrics for workflow optimization

Auto generated summary reports reporting key metrics such as volumes dispensed, concentration levels, cell isolation success rates and cassette consumption. Gain valuable insights to optimize your workflows and ensure consistent, high-quality results in your experiments.

Duo Dispensehead Cassettes

The Duo Dispensehead Cassettes enables liquid dispensing of cell suspension for cell isolation and small molecules and biomolecules in DMSO or aqueous solutions.

C1a+ cell dispensing cassette

Cassette for dispensing cell suspension.

T8+ and D4+ cassettes

Cassette for dispensing both DMSO and aqueous solutions with surfactant.
Dispensing capabilities are unchanged and backwards compatible with the D300e.

T8 surfactant-free and D4 surfactant-free cassettes

Cassette for dispensing aqueous solutions and buffers. No addition of surfactant required.
Only compatible with the Duo Digital Dispenser.

Duo Dispensehead Cassettes specification


	C1a+ cassette	T8+ cassette	D4+ cassette	T8 surfactant-free	D4 surfactant-free
Qualified fluid classes	Cell suspension	DMSO, Acetonitrile, aqueous solutions (requires surfactant), master mixes (requires surfactant)		Acetonitrile, proteomics solutions (such as trypsin, Iodoacetamide DTT, TCEP or TEAB) master mixes
Intended use	Isolation of 1 to 25 cells per well	Titration, low volume dispensing	Normalization of aqueous and DMSO	Titration, low volume dispensing	Normalization of aqueous and DMSO
Dispenseheads per cassette	1	8	4	8	4
Fill cup size	20 µl	20 µl	200 µl	20 µl	200 µl
Minimum dispense volume	480 pl	11 pl	1nL	7 pl	1nL
CV	n/a	< 8%*	< 8%*	< 8%**	< 8%**

*) For volumes > 100 pl and test fluid of 100% DMSO without other additives or for volumes > 100 pl and aqueous test fluid of 0.1% Brij 35 without other additives.
**) For volumes > 100 pl and aqueous test fluid of 100 mM PBS without other additives.

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Duo Digital Dispenser™

Picoliter dispensing redefined, now for single cells too. Meet Duo Digital Dispenser, the evolution of D300e.