Quantification and normalization

Accurate nucleic acid quantification and quality control without sample loss

Module for Fluent® automation workstation specifications and typical performance values

Software to help you normalize your DNA samples to uniform concentration

Nucleic acid quantification and normalization is required in most genomics workflows, most commonly after the initial nucleic acid purification step, but consumes precious sample and can be tedious when performed manually. The versatility of the classical UV absorbance-based technique still makes it the ‘go to’ method for many applications, as it allows the concentration of all DNA and RNA types to be measured (based on the specific empirical extinction coefficient for each nucleic acid type) and provides simultaneous assessment of samples for impurities that could impair downstream applications.

The Frida Reader is an add-on module for the Fluent Automation Workstation that revolutionizes the way quantification and normalization are performed. It allows samples to be measured in a hanging drop at the end of a disposable pipette tip, completely eliminating sample loss for nucleic acid quantification.

DNA quantification and normalization are critical steps for a wide range of genomics applications, such as next generation sequencing and quantitative PCR. Due to the limitations of classical absorbance techniques, a number of more sensitive methods for quantification of double stranded DNA (dsDNA) have been developed over the years. Fluorescence-based techniques have proved popular with researchers, as the introduction of fluorescent dsDNA-binding dyes with minimal binding to single stranded DNA (ssDNA) and RNA allows selective quantification of even small amounts of dsDNA.

Applicability of the NanoQuant PlateTM for Fluorescence Measurements.

Absorbance measurement on the Infinite® M200 microplate reader using the NanoQuant Plate™.

NanoQuant Plate™ for use on Infinite® F200 and Infinite® M200

Straightforward automation on the Tecan Freedom EVO®

Quantification and normalization of double stranded DNA with minimal set-up time.

Fast and simple DNA quantification and normalization

Fluorescence intentsity on Infinite™ F200 an Infinite M200.

Comparing absorbance measurements between the Quad4 Monochromators™-based Infinite® M200 PRO and a multimode reader using photodiode array technology.

NanoQuant Plate™ for use of Infinite® 200 NanoQuant.

Automated QC analysis for NGS Library Preparation and CRISPR/CAS9 Gene Editing

The Frida Reader for Fluent offers UV-based concentration and purity measurements of nucleic acid samples in a hanging drop at the end of a disposable tip. This patented method is completely free of sample loss, as the hanging drop is aspirated back into the tip, and can be used for further processing (e.g. a normalization). No additional preparation steps, labware or reagents are required for the measurement, which offers precision and accuracy comparable to a reference reader over a range of 2 to 1,000 ng/μl.