What equipment is required or will be useful?
Required equipment includes a microcentrifuge, pipettes, vortexer, a thermal cycler, a spectrophotometer and a magnetic plate for 0.2 mL tubes, strips, or plates. An Agilent Bioanalyzer or Fragment Analyzer may also be useful for optional analytical tests. A system for fragmentation, such as a Covaris system, is required for use with most downstream library preparations.
Do you have a kit that can be used for qPCR, archiving and NGS?
The Ovation RNA-Seq System V2 generates ds-cDNA that can be used for all three purposes.
Does Ovation RNA-Seq System V2 deplete ribosomal RNA?
Ovation RNA-Seq System V2 does not selectively deplete ribosomal RNA. You may, however, observe a reduction in the amount of ribosomal RNA content with this system when used on mammalian samples.
Can contaminating genomic DNA interfere with Ovation RNA‑Seq System V2 performance?
Yes. This kit is designed to amplify RNA, but contaminating genomic DNA may amplify during the process. For this reason we recommend DNase treatment during RNA purification.