Can I substitute my own fragmentation solution or use a mechanical/sonication method to fragment my sample?
The fragmentation cocktail has been optimized for use with downstream processing steps. Substitution with other fragmentation methods is not recommended.
Can I take the sample pools off the thermocycler to add the TX2 enzyme mix?
It is critical that the temperature is maintained at 60 °C while adding the Target Extension Enzyme Mix (TX2) to the hybridization. Mix thoroughly at this step prior to advancing to the 72 °C incubation.
Where can I stop during the protocol?
You may stop after the bead purification and/or sample concentration steps, as marked in the User Guide by a coffee cup.
Are there any special considerations when working with the adaptor plate?
Store the adaptor plate at –20 °C and keep on ice at all times, even when thawing the adaptor mixes. It is best practice to re-seal the wells after use to minimize the risk of any cross-contamination.