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Nucleic acid purification

KEY PROCESS FOR ALL GENOMICS WORKFLOWS

Extraction of nucleic acids from a wide variety of sample types is an integral part of all genomic workflows.

Tecan is working globally with collaboration partners, including Zymo Research, MACHEREY-NAGEL, Promega and Omega-Biotek to develop automated DNA/RNA extraction and clean-up solutions to match your sample types and throughput needs.

Visit our solutions related to COVID-19

Visit our DreamPrep™ NAP workstation featuring Zymo Research

List of available protocols for the Fluent and Freedom EVO® NAP workstation




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Tab 01 / Magnetic bead-based
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Magnetic bead-based purification protocols are well suited for automation, allowing flexible processing of variable sample numbers and avoiding the need for batch processing. Methods can be adapted to increase or reduce the starting sample volume, allowing you to extract exactly the right amount of DNA or RNA needed for your experiments.

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DreamPrep NAP featuring Zymo Research

The DreamPrep NAP workstation featuring Zymo Research brings together all the hardware, software and consumables required for complete, walkaway automation of magnetic-bead based extraction workflows. Preconfigured, preprogrammed and optimized in close collaboration with Zymo Research, it offers a ready-to-go processing solution for magnetic bead-based extraction workflows with integrated quantification and normalization.

Tecan and Zymo research have co-developed optimized protocols* for:

* Please make a copy of these methods in order to customize them to accommodate and validate your workflows according to your desired intended use and laboratory.

 

DreamPrep NAP offers the following integrated quantification and normalization options:

High throughput automation

Fluent® Genomics

Fluent’s groundbreaking design delivers more capacity and increased speed for genomic workflows. This innovative solution offers excellent precision, outstanding throughput and increased walkaway times

  • Up to four 96-well plates can be purified per run
  • Options for scanning 2D-barcoded primary sample tubes and elution tubes in a 96-sample format
Learn more
Scalable_automation_Limited_Offer_special

Whether you are looking for flexible automation to process 1-96 samples with an eight-channel arm or want to speed up your purification with a 96-channel head, Tecan has an array of fully tested protocols and configurations to suit your needs, including options to extend the workflow

  • Primary tube barcode scanning and sample tracking
  • Plate reader for integrated quantification and normalization
  • Dedicated TouchTools™ interface

Smart automation

Optimized Freedom EVO® 75 configuration

If you have limited lab space and want hands-free magnetic bead-based purification of highly pure nucleic acids, this affordable benchtop workstation is the ideal solution.

  • Ready-to-run protocols for genomic DNA from whole blood, human or animal tissue, cultured cells and bacteria
  • Dedicated TouchTools™ interface
Download flyer to discover more




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Tab 02 / Vacuum-based
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Solid phase nucleic acid purification (NAP) protocols allow fast processing with an integrated vacuum manifold, in either 96-well formats or 8-sample strips.

Smart automation

Optimized Freedom EVO® 75 configuration

If you have limited lab space and want hands-free vacuum-based isolation of highly pure nucleic acids, this affordable bench top workstation is the ideal solution.

  • Ready to run protocols for genomic DNA from food and feed, human or animal tissue, cultures cells and bacteria
  • Dedicated TouchTools™ interface
Download flyer to discover more

Scalable automation

Whether you are looking for flexible automation to process 1-96 with an eight-channel arm or want to speed up your purification with a 96-channel head, Tecan has an array of fully tested protocols and configurations to suit your needs, including options to extend the workflow.

  • Primary tube barcode scanning and sample tracking
  • Plate reader for integrated quantification and normalization
  • Dedicated TouchTools™ interface




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Tab 03 / Large volume gDna
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Large volume gDNA extraction with the Promega Reliaprep™ large volume HT gDNA isolation system

Freedom EVO® HSM Workstation

Promega and Tecan are partnering to provide a uniquely efficient and reliable system for isolating genomic DNA from large volumes (1-10 ml) of blood, typically in 10 ml Vacutainer® tubes. The Freedom EVO HSM and Reliaprep Large Volume HT gDNA Kit allows automated recovery of pure gDNA from up to 32 samples.

1 - Compact Instrument:
      - Freedom EVO 100 on support table
      - 120 cm (w) x 110 cm (d) x 190 cm (h)
2 - Promega HSM 2.0 (heater, shaker, magnet) integration
3 - Liquid system for fast bulk reagent dispensing
4 - Aspiration system for fast removal of supernatant

Freedom EVO® HSM Workstation

Intelligent one tube extraction – scalable for each individual sample

  • Any sample volume – from 1 to 10 ml – can be processed in a single run, using the same method
  • Single tube extraction with the ReliaPrep HSM device – combining heating, shaking and magnetic separation – reduces waste and minimizes the risk of cross contamination

Highly pure gDNA for all your downstream needs

  • From whole blood, buffy coat, buccal wash and saliva
  • Similar yields from fresh and frozen samples
  • High molecular weight DNA, ideally suited to applications requiring longer fragments, such as NGS
  • High purity specifications: A260/280 = <1.7, A260/230 = 1.8-2.2

Intuitive set-up with interactive touchscreen

  • Pre-installed protocols allow you to begin processing your samples within days
  • Easy, guided set-up minimizes training needs for laboratory staff
  • Customizable reports generated for each purification run

Tecan Journal Articles

The yield and quality of the DNA extracted has been significantly improved, which is an unexpected but very welcome benefit.

Manual DNA extraction of up to 32 large volume samples could take the entire day; with the Freedom EVO HSM it is completed in about four hours.




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Tab 04 / Literature
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Nucleic acid purification - Brochures

The DreamPrep NAP workstation featuring Zymo Research can simplify your nucleic acid extraction workflows,

today and in the future. Preconfigured, preprogrammed and optimized in close collaboration with Zymo Research,

it offers a ready-to-go processing solution for magnetic bead-based extraction workflows with integrated quantification and normalization.

The DreamPrep NAP workstation featuring Zymo Research can simplify your nucleic acid extraction workflows – today and in the future. It offers an optimized example workflow for the ZymoBIOMICS 96 MagBead DNA Kit, developed and verified in close collaboration with Zymo Research.

Need to free up time and increase productivity? Tecan’s Freedom EVO workstations are fully scalable, flexible systems that are ideally suited to nucleic acid purification and reaction set-up for a wide range of downstream applications.

EFFICIENT gDNA EXTRACTION WITH THE PROMEGA RELIAPREP™ LARGE VOLUME HT gDNA ISOLATION SYSTEM

Efficient gDNA extraction with the Promega ReliaPrep™ Large Volume HT gDNA Isolation System – tailored to your DNA biobanking needs Tecan and Promega are partnering to provide a uniquely efficient and reliable system for isolating genomic DNA from large volumes (1-10 ml) of blood, typically drawn into a standard 10 ml Vacutainer tube. The combination of the Freedom EVO, the ReliaPrep™ HSM and the ReliaPrep™ Large Volume HT gDNA Kit allows automated recovery of pure gDNA from up to 32 blood samples (each up to 10ml) in four hours.

Programming and running a Nucleic Acid Purification workflow can be done with greater speed, simplicity and process security using the intuitive TouchTools touchscreen interface from Tecan and the customizable TouchTools NAP wizard for magnetic bead or vacuum-based nucleic acid purification protocols.

Nucleic acid purification - User Guide

The DreamPrep NAP workstation featuring Zymo Research solution is based on the Fluent® 480 Automation Workstation in combination with Fluent Gx Assurance Software. The system is configured for nucleic acid extraction workflows using magnetic bead-based procedures.

Nucleic acid purification workflows have been developed and tested in collaboration with Zymo Research to deliver preprogrammed nucleic acid extraction workflows using Quick-DNA MagBead Plus Kit, ZymoBIOMICS 96 MagBead DNA Kit and Quick-DNA/RNA Viral MagBead Kit. This set-up allows processing of 1 to 96 samples at a time, with optional barcode scanning for samples and reagents.

Optional quantification and normalization of nucleic acid content is easily achieved with integrated reader options.

Magnetic bead protocols - Application Notes

Nucleic acid quantification and normalization is required in most genomics workflows, most commonly after the initial nucleic acid purification step, but consumes precious sample and can be tedious when performed manually. The versatility of the classical UV absorbance-based technique still makes it the ‘go to’ method for many applications, as it allows the concentration of all DNA and RNA types to be measured (based on the specific empirical extinction coefficient for each nucleic acid type) and provides simultaneous assessment of samples for impurities that could impair downstream applications.

The Frida Reader is an add-on module for the Fluent Automation Workstation that revolutionizes the way quantification and normalization are performed. It allows samples to be measured in a hanging drop at the end of a disposable pipette tip, completely eliminating sample loss for nucleic acid quantification.

The automated workflow for the Quick-DNA MagBead Plus Kit on the DreamPrep NAP workstation featuring Zymo Research provides a complete, walkaway DNA extraction solution from a variety of sample types, such as blood, saliva, tissue and cultured cells. It offers consistent and reproducible DNA recovery, with purities comparable to manual extraction. The results presented in this application note demonstrate that the workflow eliminates cross-contamination, generating ultra-pure DNA that can be used for any downstream application, including qPCR and NGS sequencing.

The DreamPrep NAP workstation featuring Zymo Research offers complete walkaway extraction of high quality, sequencing-grade DNA from a wide variety of microbiome sample types, providing an effective solution for reliable, high throughput and hands-free DNA purification. This plug-and-play solution delivers unbiased, consistent and reproducible DNA yields and purities, with no cross-contamination observed. The data presented in this application note demonstrates the successful recovery, excellent reproducibility and consistency between sample preparations when evaluating the yield, concentration, and purity of the extracted DNA.

The DreamPrep NAP workstation featuring Zymo Research offers complete walkaway extraction of viral DNA and RNA from plasma or serum samples. The results presented in this application note demonstrate efficient recovery of viral DNA and RNA in a consistent and reproducible manner. The automated extraction workflow is equivalent to manual extraction in terms of total nucleic acid recovery, successfully extracting viral RNA from samples containing as little as 60 viral copies, with lower CT values. This workflow shows no cross-contamination and can achieve high quality viral DNA from a variety of samples (eg. plasma and serum). The resulting viral RNA can be used for sensitive downstream applications, such as RT/qPCR and next generation sequencing.

Extraction of genomic DNA from the whole blood is a common first step for a variety of downstream applications such as PCR or next generation sequencing protocols. The Maxwell HT 96 gDNA Blood Isolation System (Promega) offers rapid isolation of genomic DNA from whole blood based on magnetic beads technology. The protocol is fully automated on a Fluent Automation Workstation with FluentControl™ software which together with TouchTools™ user interface (UI) and Smart Commands ensure reduced complexity and flexibility of the workflow set up. As a result, the automated run delivers consistently high and uniform DNA yields in a reliable and reproducible manner, with minimal hands-on time.

Extraction of plasmid DNA is a standard procedure in molecular biology laboratories around the world, and plasmids are still a go-to resource for cloning and modifying genes of interest. For protein production applications, large volume bacterial cultures are often required, creating a need for efficient purification techniques capable of generating large quantities of the plasmid of interest. Promega’s Wizard MagneSil Tfx System automated on the Freedom EVO workstation offers a rapid and simple method for isolating transfection-quality plasmid DNA in a high throughput format to establish a flexible, fully automated solution for the purification of plasmid DNA, streamlining laboratory workflows and providing fast and reliable extraction of high purity DNA.

There is increasing demand to purify genomic DNA (gDNA) from human blood and saliva at small scale and high throughput using liquid handling platforms. Promega’s ReliaPrep 96 gDNA Miniprep HT System is designed for automated recovery of gDNA from 100-350 μl of human whole blood or 350-700 μl of human saliva, for research use only, not for use in clinical diagnostics. Full automation of the gDNA purification process on a Freedom EVO® workstation streamlines laboratory workflows and provides reliable, rapid extraction of highly pure gDNA.

Tecan and MACHERY-NAGEL have joined forces to provide a flexible automated solution for the isolation of genomic plant DNA without compromising yield or purity. After the initial homogenization of the plant material, the workflow can be completely automated on a Freedom EVO sample preparation workstation, reducing the risk of contamination, carry-over and manual errors to a minimum. Processing time is only 50 min for up to 384 samples of plant extracts.

Fast and reliable DNA extraction from plants with NucleoMag® 96 Plant from MACHEREY-NAGEL on a Freedom EVO® platform.

High yields and excellent purity viral nucleic acid extraction with the MACHEREY-NAGEL NucleoMag® 96 Virus kit on a Freedom EVO® platform.

PCR has become a standard molecular biology tool for biological and medical research laboratories, and is used for DNA cloning, sequencing and genetic testing. For most applications, PCR products need to be purified, and this is advantages to perform in a high throughput format. Tecan and MACHEREY-NAGEL have joined forces to provide a flexible automated solution for the purification of PCR products for research use only, not for use in clinical diagnostics.

Tissue gDNA extraction with MACHEREY-NAGEL’s NucleoMag® 96 Tissue kit and the Freedom EVO® workstation

Extraction of RNA from cells and tissue with high yield and purity with the NucleoMag® 96 RNA kit on a Freedom EVO® platform.

The rising challenges of global food production are leading to the increased use of genetically modified organisms (GMOs) in food and feed. Moreover, there is increasing need for the reliable detection of foodborne pathogens, food adulterations and allergens, which requires tailored and optimized analytical methods.

MACHEREY-NAGEL and Tecan have partnered to deliver a tailored solution for high throughput DNA extraction from food and feed sample material using the NucleoMag DNA Food kit on the Freedom EVO 150, offering:

  • Reliable performance and excellent DNA yields for species identification and GMO detection
  • Excellent recovery from diverse and challenging food matrices
  • Tailored protocol for processing variable sample numbers

The isolation of viral RNA and DNA or microbial DNA is often tedious for routine laboratories, as different starting materials require alternative purification methods. The molecular diagnostic market demands extraction methods that are adaptable on automation platforms, and reliable in terms of pathogen DNA/RNA detection1. Moreover, the purification process needs to be suitable for a wide variety of sample materials.

MACHEREY-NAGEL and Tecan have partnered to deliver a tailored solution for high throughput extraction of viral RNA and DNA, and microbial DNA from clinical sample materials. The NucleoMag Pathogen procedure has been implemented on the Freedom EVO 150 system to automate the nucleic acid purification workflow, offering:

  • Excellent recovery of nucleic acids from diverse clinical sample material
  • Carrier RNA, proteinase K, and ready-to-use buffers allowing you to start right away
  • Tailored protocol for processing variable sample numbers

Vaccuum and positive pressure-based protocols - Application Notes

Combining the Quick-RNA Viral 96 Kit workflow with the Resolvex A200 workstation enables high throughput viral RNA extraction that is ideal for SARS-CoV-2 testing. The workflow reduces hands-on processing time and the use of consumables. This application note describes the semi-automated extraction workflow for SARS-CoV-2 viral RNA extraction using the Quick-RNA Viral 96 kit on the Resolvex A200 positive pressure system.

The global COVID-19 pandemic has resulted in a rapid increase in demand for the isolation of viral RNA. The NucleoSpin 96 Virus kit from MACHEREY-NAGEL is designed for the extraction of viral nucleic acids – RNA and DNA viruses – from cell-free, biological fluids, such as saliva, urine, serum or plasma, and sample homogenates (particle-free supernatants), for example, from swabs, tissue or stool. Combining the NucleoSpin 96 plate-based workflow with the Resolvex A200 workstation enables processing of silica membrane extraction plates under positive pressure, offering an accessible semi-automated solution to rapidly scale up throughput and reduce hands-on time.

Extraction of plasmid DNA using the QIAprep 96 Turbo and QIAprep 96 Plus protocols on the Resolvex A200 positive pressure workstation results in high purities and comparable yields to the reference methods demonstrating the suitability of this system for routine use in high-throughput labs. The faster QIAprep 96 Turbo protocol reduces sample transfer steps and hand-on time but results in lower yields compared to the QIAprep 96 Plus protocol. The QIAprep 96 Plus protocol delivers higher plasmid DNA yields with lower variation, but it requires additional sample handling steps. The Resolvex A200 workflow reduces handling times and errors compared to the reference method by providing direct dispensing of all buffers and consistent pressure gradients.

AGRANIS is a service laboratory in Laval, France, that specializes in milk sample analysis using an innovative automated technique for determining the somatic cell count (SCC) of each cow in a herd from a bulk tank milk sample called GénoCellules®. The SCC is quantified as the number of cells/ml of milk, and is used as an indicator of quality. Generally, the SCC is determined by flow cytometry, requiring analysis of individual milk samples from each cow in a herd, which involves a large number of samples. However, prior genotyping of cartilage samples from each cow in a herd makes it possible to subsequently determine the SCC of each cow in a single bulk tank milk sample.

As part of this process AGRANIS uses Nucleospin Tissue 96 kits (Macherey-Nagel) for DNA extraction of both cartilage and bulk milk samples on a Fluent® Automation Workstation equipped with an eight-channel Air FCA™, a Robotic Gripper Arm™, a BioShake® 3000-T elm (Quantifoil Instruments) for heating, shaking and homogenization of samples, and a Te-VacS™ module with a customer-specific suction force profile to optimize the process. The automated protocol has enabled AGRANIS to carry out twice as many analyses in the same time as with their previous approach. The enhanced speed enables reliable batch processing of 96 samples in just two hours, and several plates per day.

Rapid extraction of genomic DNA from a variety of food and feed samples with NucleoSpin® 8/96 Food columns and the Freedom EVO®

Reliable extraction of exceptional purity plasmid DNA using the NucleoSpin® 96 Plasmid kit on a on a Freedom EVO® platform

Reliable extraction of plant DNA using the NucleoSpin® Plant II Midi kit from MACHEREY-NAGEL on a Freedom EVO® platform

Reliable extraction of gDNA from blood samples with high yield and purity using the NucleoSpin® 96 Blood kit on a Freedom EVO® platform.

Reliable extraction of gDNA from cells and tissue samples with high yield and purity using the NucleoSpin® 96 Tissue kit on a Freedom EVO® platform

Reliable extraction of RNA from cells and tissue with high yield and purity using the NucleoSpin® 96 RNA kit on a Freedom EVO® platform

Reliable purification of PCR products with high yield and purity using the MACHEREY-NAGEL NucleoSpin® 96 PCR Clean-up kit on a Freedom EVO® platform

DNA extraction from various tissue samples comes with specific matrix challenges, and can be elaborate and time consuming for samples such as mouse tail, mammalian organs and eukaryotic cells. Efficient sample lysis and DNA release is essential for downstream molecular applications used by many research laboratories.

MACHEREY NAGEL and Tecan have partnered to deliver an automated solution for high throughput DNA extraction from various tissue samples using the NucleoSpin 96 DNA RapidLyse kit on the Freedom EVO 150, offering:

  • Reliable performance and excellent yields
  • An optimized protocol allowing flexible sample numbers in multiples of eight samples
  • Fast processing of 96 samples within 90 minutes (excluding lysis)
For research use only. Not for use in clinical diagnostic procedures