Nucleic acid purification

Need to free up time and increase productivity? Tecan’s Freedom EVO workstations are fully scalable, flexible systems that are ideally suited to nucleic acid purification and reaction set-up for a wide range of downstream applications.

EFFICIENT gDNA EXTRACTION WITH THE PROMEGA RELIAPREP™ LARGE VOLUME HT gDNA ISOLATION SYSTEM

Efficient gDNA extraction with the Promega ReliaPrep™ Large Volume HT gDNA Isolation System – tailored to your DNA biobanking needs Tecan and Promega are partnering to provide a uniquely efficient and reliable system for isolating genomic DNA from large volumes (1-10 ml) of blood, typically drawn into a standard 10 ml Vacutainer tube. The combination of the Freedom EVO, the ReliaPrep™ HSM and the ReliaPrep™ Large Volume HT gDNA Kit allows automated recovery of pure gDNA from up to 32 blood samples (each up to 10ml) in four hours.

Programming and running a Nucleic Acid Purification workflow can be done with greater speed, simplicity and process security using the intuitive TouchTools touchscreen interface from Tecan and the customizable TouchTools NAP wizard for magnetic bead or vacuum-based nucleic acid purification protocols.

The automated workflow for the Quick-DNA MagBead Plus Kit on the DreamPrep NAP workstation featuring Zymo Research provides a complete, walkaway DNA extraction solution from a variety of sample types, such as blood, saliva, tissue and cultured cells. It offers consistent and reproducible DNA recovery, with purities comparable to manual extraction. The results presented in this application note demonstrate that the workflow eliminates cross-contamination, generating ultra-pure DNA that can be used for any downstream application, including qPCR and NGS sequencing.

The DreamPrep NAP workstation featuring Zymo Research offers complete walkaway extraction of high quality, sequencing-grade DNA from a wide variety of microbiome sample types, providing an effective solution for reliable, high throughput and hands-free DNA purification. This plug-and-play solution delivers unbiased, consistent and reproducible DNA yields and purities, with no cross-contamination observed. The data presented in this application note demonstrates the successful recovery, excellent reproducibility and consistency between sample preparations when evaluating the yield, concentration, and purity of the extracted DNA.

The DreamPrep NAP workstation featuring Zymo Research offers complete walkaway extraction of viral DNA and RNA from plasma or serum samples. The results presented in this application note demonstrate efficient recovery of viral DNA and RNA in a consistent and reproducible manner. The automated extraction workflow is equivalent to manual extraction in terms of total nucleic acid recovery, successfully extracting viral RNA from samples containing as little as 60 viral copies, with lower CT values. This workflow shows no cross-contamination and can achieve high quality viral DNA from a variety of samples (eg. plasma and serum). The resulting viral RNA can be used for sensitive downstream applications, such as RT/qPCR and next generation sequencing.

Extraction of genomic DNA from the whole blood is a common first step for a variety of downstream applications such as PCR or next generation sequencing protocols. The Maxwell HT 96 gDNA Blood Isolation System (Promega) offers rapid isolation of genomic DNA from whole blood based on magnetic beads technology. The protocol is fully automated on a Fluent Automation Workstation with FluentControl™ software which together with TouchTools™ user interface (UI) and Smart Commands ensure reduced complexity and flexibility of the workflow set up. As a result, the automated run delivers consistently high and uniform DNA yields in a reliable and reproducible manner, with minimal hands-on time.

Extraction of plasmid DNA is a standard procedure in molecular biology laboratories around the world, and plasmids are still a go-to resource for cloning and modifying genes of interest. For protein production applications, large volume bacterial cultures are often required, creating a need for efficient purification techniques capable of generating large quantities of the plasmid of interest. Promega’s Wizard MagneSil Tfx System automated on the Freedom EVO workstation offers a rapid and simple method for isolating transfection-quality plasmid DNA in a high throughput format to establish a flexible, fully automated solution for the purification of plasmid DNA, streamlining laboratory workflows and providing fast and reliable extraction of high purity DNA.

There is increasing demand to purify genomic DNA (gDNA) from human blood and saliva at small scale and high throughput using liquid handling platforms. Promega’s ReliaPrep 96 gDNA Miniprep HT System is designed for automated recovery of gDNA from 100-350 μl of human whole blood or 350-700 μl of human saliva, for research use only, not for use in clinical diagnostics. Full automation of the gDNA purification process on a Freedom EVO® workstation streamlines laboratory workflows and provides reliable, rapid extraction of highly pure gDNA.

Tecan and MACHERY-NAGEL have joined forces to provide a flexible automated solution for the isolation of genomic plant DNA without compromising yield or purity. After the initial homogenization of the plant material, the workflow can be completely automated on a Freedom EVO sample preparation workstation, reducing the risk of contamination, carry-over and manual errors to a minimum. Processing time is only 50 min for up to 384 samples of plant extracts.

Fast and reliable DNA extraction from plants with NucleoMag® 96 Plant from MACHEREY-NAGEL on a Freedom EVO® platform.

High yields and excellent purity viral nucleic acid extraction with the MACHEREY-NAGEL NucleoMag® 96 Virus kit on a Freedom EVO® platform.

PCR has become a standard molecular biology tool for biological and medical research laboratories, and is used for DNA cloning, sequencing and genetic testing. For most applications, PCR products need to be purified, and this is advantages to perform in a high throughput format. Tecan and MACHEREY-NAGEL have joined forces to provide a flexible automated solution for the purification of PCR products for research use only, not for use in clinical diagnostics.

Tissue gDNA extraction with MACHEREY-NAGEL’s NucleoMag® 96 Tissue kit and the Freedom EVO® workstation

Extraction of RNA from cells and tissue with high yield and purity with the NucleoMag® 96 RNA kit on a Freedom EVO® platform.

The rising challenges of global food production are leading to the increased use of genetically modified organisms (GMOs) in food and feed. Moreover, there is increasing need for the reliable detection of foodborne pathogens, food adulterations and allergens, which requires tailored and optimized analytical methods.

MACHEREY-NAGEL and Tecan have partnered to deliver a tailored solution for high throughput DNA extraction from food and feed sample material using the NucleoMag DNA Food kit on the Freedom EVO 150, offering:

• Reliable performance and excellent DNA yields for species identification and GMO detection
• Excellent recovery from diverse and challenging food matrices
• Tailored protocol for processing variable sample numbers

The isolation of viral RNA and DNA or microbial DNA is often tedious for routine laboratories, as different starting materials require alternative purification methods. The molecular diagnostic market demands extraction methods that are adaptable on automation platforms, and reliable in terms of pathogen DNA/RNA detection1. Moreover, the purification process needs to be suitable for a wide variety of sample materials.

MACHEREY-NAGEL and Tecan have partnered to deliver a tailored solution for high throughput extraction of viral RNA and DNA, and microbial DNA from clinical sample materials. The NucleoMag Pathogen procedure has been implemented on the Freedom EVO 150 system to automate the nucleic acid purification workflow, offering:

• Excellent recovery of nucleic acids from diverse clinical sample material
• Carrier RNA, proteinase K, and ready-to-use buffers allowing you to start right away
• Tailored protocol for processing variable sample numbers

The global COVID-19 pandemic has resulted in a rapid increase in demand for the isolation of viral RNA. The NucleoSpin 96 Virus kit from MACHEREY-NAGEL is designed for the extraction of viral nucleic acids – RNA and DNA viruses – from cell-free, biological fluids, such as saliva, urine, serum or plasma, and sample homogenates (particle-free supernatants), for example, from swabs, tissue or stool. Combining the NucleoSpin 96 plate-based workflow with the Resolvex A200 workstation enables processing of silica membrane extraction plates under positive pressure, offering an accessible semi-automated solution to rapidly scale up throughput and reduce hands-on time.

Extraction of plasmid DNA using the QIAprep 96 Turbo and QIAprep 96 Plus protocols on the Resolvex A200 positive pressure workstation results in high purities and comparable yields to the reference methods demonstrating the suitability of this system for routine use in high-throughput labs. The faster QIAprep 96 Turbo protocol reduces sample transfer steps and hand-on time but results in lower yields compared to the QIAprep 96 Plus protocol. The QIAprep 96 Plus protocol delivers higher plasmid DNA yields with lower variation, but it requires additional sample handling steps. The Resolvex A200 workflow reduces handling times and errors compared to the reference method by providing direct dispensing of all buffers and consistent pressure gradients.

AGRANIS is a service laboratory in Laval, France, that specializes in milk sample analysis using an innovative automated technique for determining the somatic cell count (SCC) of each cow in a herd from a bulk tank milk sample called GénoCellules®. The SCC is quantified as the number of cells/ml of milk, and is used as an indicator of quality. Generally, the SCC is determined by flow cytometry, requiring analysis of individual milk samples from each cow in a herd, which involves a large number of samples. However, prior genotyping of cartilage samples from each cow in a herd makes it possible to subsequently determine the SCC of each cow in a single bulk tank milk sample.

As part of this process AGRANIS uses Nucleospin Tissue 96 kits (Macherey-Nagel) for DNA extraction of both cartilage and bulk milk samples on a Fluent® Automation Workstation equipped with an eight-channel Air FCA™, a Robotic Gripper Arm™, a BioShake® 3000-T elm (Quantifoil Instruments) for heating, shaking and homogenization of samples, and a Te-VacS™ module with a customer-specific suction force profile to optimize the process. The automated protocol has enabled AGRANIS to carry out twice as many analyses in the same time as with their previous approach. The enhanced speed enables reliable batch processing of 96 samples in just two hours, and several plates per day.

Rapid extraction of genomic DNA from a variety of food and feed samples with NucleoSpin® 8/96 Food columns and the Freedom EVO®

Reliable extraction of exceptional purity plasmid DNA using the NucleoSpin® 96 Plasmid kit on a on a Freedom EVO® platform

Reliable extraction of plant DNA using the NucleoSpin® Plant II Midi kit from MACHEREY-NAGEL on a Freedom EVO® platform

Reliable extraction of gDNA from blood samples with high yield and purity using the NucleoSpin® 96 Blood kit on a Freedom EVO® platform.

Reliable extraction of gDNA from cells and tissue samples with high yield and purity using the NucleoSpin® 96 Tissue kit on a Freedom EVO® platform

Reliable extraction of RNA from cells and tissue with high yield and purity using the NucleoSpin® 96 RNA kit on a Freedom EVO® platform

Reliable purification of PCR products with high yield and purity using the MACHEREY-NAGEL NucleoSpin® 96 PCR Clean-up kit on a Freedom EVO® platform

DNA extraction from various tissue samples comes with specific matrix challenges, and can be elaborate and time consuming for samples such as mouse tail, mammalian organs and eukaryotic cells. Efficient sample lysis and DNA release is essential for downstream molecular applications used by many research laboratories.

MACHEREY NAGEL and Tecan have partnered to deliver an automated solution for high throughput DNA extraction from various tissue samples using the NucleoSpin 96 DNA RapidLyse kit on the Freedom EVO 150, offering:

• Reliable performance and excellent yields
• An optimized protocol allowing flexible sample numbers in multiples of eight samples
• Fast processing of 96 samples within 90 minutes (excluding lysis)