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Nucleic acid purification

KEY PROCESS FOR ALL GENOMICS WORKFLOWS

Extraction of nucleic acids from a wide variety of sample types is an integral part of all genomic workflows.

Tecan is working globally with collaboration partners, including Zymo Research, MACHEREY-NAGEL, Promega and Omega-Biotek to develop automated DNA/RNA extraction and clean-up solutions to match your sample types and throughput needs.
DreamPrep NAP
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Tab 01 / DreamPrep NAP
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DreamPrep® NAP workstation

The DreamPrep NAP workstation brings together hardware, software and consumables required for walkaway automation of magnetic-bead based extraction workflows. It offers a ready-to-go processing solution for 1-96 samples per batch with integrated quantification and normalization.

Tecan has partnered with a variety of nucleic acid extraction providers to develop preprogrammed, optimized protocols* for different sample types and applications.

Click here to open the list of available scripts

* Please make a copy of these methods in order to customize them to accommodate and validate your workflows according to your desired intended use and laboratory.

Frida-Reader_DreamPrep-NAP_720x484

DreamPrep and Quant/Norm

DreamPrep NAP offers the following integrated quantification and normalization options:

DreamPrep NAP featuring our chemistry partners

Tecan closely collaborates with nucleic acid purification kit manufacturers to provide high quality solutions for the automated extraction of nucleic acids.




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Tab 02 / Magnetic bead-based
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Automated nucleic acid extraction methods

Magnetic bead-based

Magnetic bead-based purification protocols are well suited for automation, allowing flexible processing of variable sample numbers and avoiding the need for batch processing. Methods can be adapted to increase or reduce the starting sample volume, allowing you to extract exactly the right amount of DNA or RNA needed for your experiments.

 

Click here to open the list of available scripts

High throughput automation

Fluent® Genomics

Fluent’s groundbreaking design delivers more capacity and increased speed for genomic workflows. This innovative solution offers excellent precision, outstanding throughput and increased walkaway times

  • Up to four 96-well plates can be purified per run
  • Options for scanning 2D-barcoded primary sample tubes and elution tubes in a 96-sample format
Read more

Scalable automation

Freedom EVO® 100/150 NAP Workstations

Whether you are looking for flexible automation to process 1-96 samples with an eight-channel arm or want to speed up your purification with a 96-channel head, Tecan has an array of fully tested protocols and configurations to suit your needs, including options to extend the workflow

  • Primary tube barcode scanning and sample tracking
  • Plate reader for integrated quantification and normalization
  • Dedicated TouchTools™ interface

Smart automation

Optimized Freedom EVO 75 configuration

If you have limited lab space and want hands-free magnetic bead-based purification of highly pure nucleic acids, this affordable benchtop workstation is the ideal solution.

  • Ready-to-run protocols for genomic DNA from whole blood, human or animal tissue, cultured cells and bacteria
  • Dedicated TouchTools™ interface
Download flyer to discover more




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Tab 03 / Vacuum-based
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Automated nucleic acid extraction methods

Vacuum-based

Solid phase nucleic acid purification (NAP) protocols allow fast processing with an integrated vacuum manifold, in either 96-well formats or 8-sample strips.

 

Click here to open the list of available scripts

Smart automation

Optimized Freedom EVO® 75 configuration

If you have limited lab space and want hands-free vacuum-based isolation of highly pure nucleic acids, this affordable bench top workstation is the ideal solution.

  • Ready to run protocols for genomic DNA from food and feed, human or animal tissue, cultures cells and bacteria
  • Dedicated TouchTools™ interface
Download flyer to discover more

Scalable automation

Whether you are looking for flexible automation to process 1-96 with an eight-channel arm or want to speed up your purification with a 96-channel head, Tecan has an array of fully tested protocols and configurations to suit your needs, including options to extend the workflow.

  • Primary tube barcode scanning and sample tracking
  • Plate reader for integrated quantification and normalization
  • Dedicated TouchTools™ interface




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Tab 04 / Large volume gDna
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Large volume gDNA extraction with the Promega Reliaprep™ large volume HT gDNA isolation system

Freedom EVO® HSM Workstation

Promega and Tecan are partnering to provide a uniquely efficient and reliable system for isolating genomic DNA from large volumes (1-10 ml) of blood, typically in 10 ml Vacutainer® tubes. The Freedom EVO HSM and Reliaprep Large Volume HT gDNA Kit allows automated recovery of pure gDNA from up to 32 samples.

1 - Compact Instrument:
      - Freedom EVO 100 on support table
      - 120 cm (w) x 110 cm (d) x 190 cm (h)
2 - Promega HSM 2.0 (heater, shaker, magnet) integration
3 - Liquid system for fast bulk reagent dispensing
4 - Aspiration system for fast removal of supernatant

Freedom EVO® HSM Workstation

Intelligent one tube extraction – scalable for each individual sample

  • Any sample volume – from 1 to 10 ml – can be processed in a single run, using the same method
  • Single tube extraction with the ReliaPrep HSM device – combining heating, shaking and magnetic separation – reduces waste and minimizes the risk of cross contamination

Highly pure gDNA for all your downstream needs

  • From whole blood, buffy coat, buccal wash and saliva
  • Similar yields from fresh and frozen samples
  • High molecular weight DNA, ideally suited to applications requiring longer fragments, such as NGS
  • High purity specifications: A260/280 = <1.7, A260/230 = 1.8-2.2

Intuitive set-up with interactive touchscreen

  • Pre-installed protocols allow you to begin processing your samples within days
  • Easy, guided set-up minimizes training needs for laboratory staff
  • Customizable reports generated for each purification run

Tecan Journal Articles

Futureproofing biobanking procedures
[TJ 02/2016]

The yield and quality of the DNA extracted has been significantly improved, which is an unexpected but very welcome benefit.

Incorporating genomic medicine into cancer research and primary care
[TJ 03/2014]

Manual DNA extraction of up to 32 large volume samples could take the entire day; with the Freedom EVO HSM it is completed in about four hours.




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Tab 05 / Literature
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