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Unique RNA-Seq library prep combines three proprietary technologies to provide a streamlined solution for whole transcriptome RNA-Seq from low input and poor quality samples for rare transcript detection and unbiased pathogen discovery.
Looking for a whole transcriptome RNA-Seq solution similar to Trio RNA-Seq?
Check out Revelo™ RNA-Seq High Sensitivity for our new and improved RNA-Seq library preparation technology.
RNA sequencing library preparation from challenging or mixed samples, where detection of low abundance or rare transcripts is critical, is a serious challenge to many researchers. Standard RNA-Seq methods are not adequate for the generation of templates suitable for sequencing rare transcripts in mixed samples. Standard RNA-Seq kits are unable to detect viral pathogens such as SARS-CoV-2 or identify uniquely expressing genes in specific cell types from challenging tissues such as neurobiological samples.
Trio’s unique amplification technology (single primer isothermal amplification, SPIA) allows for the detection of low input amounts and the low abundance of pathogens like viruses. This unbiased amplification technique generates more molecules for library preparation compared to standard RNA-Seq workflows and preserves biological information from samples. SPIA can tolerate inhibitors from challenging sample sources enabling better amplification of your transcript of interest.
Trio RNA-Seq offers a best-in-class and highly published NGS library preparation system that combines SPIA with DimerFree adaptor ligation and AnyDeplete targeted transcript depletion, offering a streamlined solution for whole transcriptome RNA-Seq from low input and poor quality samples for rare transcript detection and unbiased pathogen discovery.
Trio low input RNA-Seq is offered with both AnyDeplete probes targeting human rRNA (Part No. 0506) and AnyDeplete probes targeting mouse rRNA (Part No. 0507). AnyDeplete probe sets can be customized to any transcript from any organism. For custom probe sets, contact your Account Executive or request a quote on our website.
Robust DNA-Seq library prep kit for a broad range of sample types starting with as little as...
DNA-Seq library prep kit with optimization free enzymatic fragmentation for a 3 step...
Revelo RNA-seq High Sensitivity library preparation solutions combine proprietary technologies...
For research use only. Not for use in diagnostic procedures.