Tecan uses cookies to improve our website. By continuing to browse our website, you accept our cookie policy.

This content is available also in other languages

Close
x

This content is available also in other languages

    Trio RNA-Seq™ library preparation kit

    Unique RNA-Seq library prep combines three proprietary technologies to provide a streamlined solution for whole transcriptome RNA-Seq from low input and poor quality samples for rare transcript detection and unbiased pathogen discovery.
    Overview
    Go to Shop     Request Pricing Talk to an expert




    ***************************************************************************************
    ***************************************************************************************
    Tab 01 / Overview
    ***************************************************************************************
    ***************************************************************************************

    Product News: Trio RNA-Seq is no longer available.

    Looking for a whole transcriptome RNA-Seq solution similar to Trio RNA-Seq?

    Check out Revelo™ RNA-Seq High Sensitivity for our new and improved RNA-Seq library preparation technology.

     

    Selection guide

    Sensitive detection of rare transcripts from challenging samples

    RNA sequencing library preparation from challenging or mixed samples, where detection of low abundance or rare transcripts is critical, is a serious challenge to many researchers. Standard RNA-Seq methods are not adequate for the generation of templates suitable for sequencing rare transcripts in mixed samples. Standard RNA-Seq kits are unable to detect viral pathogens such as SARS-CoV-2 or identify uniquely expressing genes in specific cell types from challenging tissues such as neurobiological samples.

    Trio’s unique amplification technology (single primer isothermal amplification, SPIA) allows for the detection of low input amounts and the low abundance of pathogens like viruses. This unbiased amplification technique generates more molecules for library preparation compared to standard RNA-Seq workflows and preserves biological information from samples. SPIA can tolerate inhibitors from challenging sample sources enabling better amplification of your transcript of interest.

    Trio RNA-Seq offers a best-in-class and highly published NGS library preparation system that combines SPIA with DimerFree adaptor ligation and AnyDeplete targeted transcript depletion, offering a streamlined solution for whole transcriptome RNA-Seq from low input and poor quality samples for rare transcript detection and unbiased pathogen discovery.

     

    Benefits of Trio RNA-Seq library preparation kit:

    • RNA sequencing solution for liquid biopsy, FFPE, nasal swab and other challenging samples with low input.
    • Customize rRNA depletion after library construction maximizing informative sequencing reads from whole transcriptome data.
    • Enzymatic fragmentation and DimerFree library construction allowing efficient and robust library preparation.
    • Detect index hopping with UDI.
    • Input range 500 pg - 50 ng of total RNA.
    • Ideal for detection and characterization of COVID-19 and infectious disease agents.

    Trio low input RNA-Seq is offered with both AnyDeplete probes targeting human rRNA (Part No. 0506) and AnyDeplete probes targeting mouse rRNA (Part No. 0507). AnyDeplete probe sets can be customized to any transcript from any organism. For custom probe sets, contact your Account Executive or request a quote on our website.

    Applications

    • RNA sequencing (RNA-Seq)
    • Whole transcriptome profiling
    • Gene expression
    • Transcript discovery
    • Viral detection




    ***************************************************************************************
    ***************************************************************************************
    Tab 02 / Literature
    ***************************************************************************************
    ***************************************************************************************

    Resources

    Type
    Title
    No
    Related products
    Ovation® Ultralow V2 DNA-Seq Library Preparation Kit
    Ovation® Ultralow V2 DNA-Seq Library Preparation Kit

    Robust DNA-Seq library prep kit for a broad range of sample types starting with as little as...

    Celero™ DNA-Seq Library Prep
    Celero™ DNA-Seq Library Prep

    DNA-Seq library prep kit with optimization free enzymatic fragmentation for a 3 step...

    Revelo™ RNA-Seq High Sensitivity library preparation kit
    Revelo™ RNA-Seq High Sensitivity library preparation kit

    Revelo RNA-seq High Sensitivity library preparation solutions combine proprietary technologies...

    Product groups

     

    Areas of interest
    Resources

     

    Contact & Support

    For research use only. Not for use in diagnostic procedures.
    empty cart | close cart
    Your cart

    Your cart is empty.

     
    Update totals

    Total:

     

    Get a quote close form

     

     

     

    |delete item