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Product selection guide

Find the right product for your experiment.

For further questions regarding our kits contact Tecan's NGS scientific support.

DNA-Seq solutions

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Input Amount		10ng – 500ng	10ng – 500ng	200ng – 500ng	10pg – 100ng	10ng – 100ng
Input Type		High quality gDNA	High quality gDNA	High quality gDNA	High Quality gDNA, FFPE, cfDNA or degraded DNA	High quality gDNA
Enzymatic Fragmentation		no	yes	yes	no	yes
PCR-Free		no	no	yes	no	no
Unique Dual Index		yes	yes	yes	yes	no
High-throughput Multiplexing		yes	yes	no	no	yes
Molecular Tags		no	no	no	yes	no
Custom Target Enrichment Probes		no	no	no	no	yes
NuQuant®		yes	yes	no	no	no
		Celero™ DNA-Seq	Celero™ EZ DNA-Seq	Rapid EZ DNA-Seq™	Ovation Ultralow DNA-Seq V2	Allegro® Targeted Genotyping V2

DNA-Seq_Selection_Guide-min

RNA-Seq solutions

Standard input library preparation solutions for Illumina sequencing.

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Input Amount	10ng - 1µg	10ng - 500ng	100ng - 500ng	10ng - 250ng
Input Type	High quality total RNA	High quality, FFPE or degraded total RNA	High quality or degraded bacterial RNA	High quality, FFPE or degraded total RNA
Chemical Fragmentation	yes	yes	no	no
Strand Specific	yes	yes	yes	yes
Targeted Depletion	yes	yes	yes	yes
Molecular Tags	yes	yes	yes	yes
Up to 192 Unique Dual Indexes	yes	yes	yes	yes
NuQuant®	yes	yes	no	yes
Platform Compatibility	Illumina	Illumina	Illumina	Illumina
	Universal Plus mRNA-Seq with NuQuant	Universal Plus Total RNA-Seq with NuQuant	Universal Prokaryotic RNA-Seq	Universal RNA-Seq with NuQuant

Standard input library preparation solutions for Illumina sequencing.

RNA-Seq solutions

Ultra-low input library preparation solutions for Illumina sequencing.

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Input Amount	10pg - 10ng	500pg - 50ng	500pg - 100ng	250pg - 10ng
Input Type	High quality, FFPE, cf RNA or degraded total RNA	High quality, FFPE or degraded total RNA	High quality, FFPE or degraded total RNA	High quality, nasal swabs or degraded total RNA
Isothermal Amplification	no	yes	yes	yes
Strand Specific	yes	no	no	no
Targeted Depletion	yes	yes	no	yes*
Molecular Tags	yes	no	no	no
Up to 96 Unique Dual Indexes	no	yes	no	yes
NuQuant®	no	no	no	yes
Platform Compatibility	Illumina	Illumina	Illumina Ion	Illumina
	Ovation® SoLo® RNA-Seq System	Trio RNA-Seq	Ovation RNA-Seq System V2	Revelo™ RNA-Seq
Separate Library Prep Kit Required			Ovation Ultralow System V2	*human rRNA only

Ultra-low input library preparation solutions for Illumina sequencing. style=

Methyl-Seq solutions

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Input Amount	10ng – 300ng	100ng
Input Type	High quality, FFPE, cell-free or degraded DNA	High quality genomic DNA
Enzymatic Fragmentation	no	yes
Directional Libraries	yes	yes
Diversity Adaptors	no	yes
Molecular Tag	no	yes
Oxidative Bisulfite Reagents Included	yes	yes
Up to 96 barcodes	yes	yes
Automation-ready	yes	yes
Platform Compatibility	Illumina	Illumina
	Ultralow Methyl-Seq™ with TrueMethyl® oxBS	Ovation® RRBS Methyl-Seq with TrueMethyl® oxBS

PSG_04_Methyl-Seq_solutions

RNA sample preparation

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	Starting materials and sample sizes
Platform	Good Quality >50 ng	Good Quality 5–50 ng	Good Quality & Degraded 500 pg–50 ng	Whole Blood 5–50 ng	FFPE 50 ng
Affymetrix GeneChip® 3’ Arrays	Ovation® RNA Amplification System V2 Encore Biotin Module	Ovation RNA Amp System V2 Encore Biotin Module	Ovation Pico WTA System V2 Encore Biotin Module	Ovation RNA Amp System V2 Ovation WB Reagent Encore Biotin Module	Ovation FFPE WTA System Encore Biotin Module
Affymetrix GeneChip Exon/Gene ST Arrays		Ovation Pico WTA System V2 Ovation PicoSL WTA System V2 Encore Biotin Module	Ovation Pico WTA System V2 Ovation PicoSL WTA System V2 Encore Biotin Module	Ovation Pico WTA System V2 Ovation PicoSL WTA System V2 Encore Biotin Module	Ovation FFPE WTA System Encore Biotin Module
Agilent Dual-Mode Gene Expression Arrays	Ovation RNA Amplification System V2	Ovation RNA Amp System V2	Ovation PicoSL WTA System V2	Ovation RNA Amp System V2	Ovation FFPE WTA System*
Quantitative PCR		Crescendo cDNA Synthesis for qPCR	Crescendo cDNA Synthesis for qPCR	Crescendo cDNA Synthesis for qPCR	Crescendo cDNA Synthesis for qPCR
*May require optimization of fragment sizes

qPCR Product Matrix