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Product selection guide

Find the right product for your experiment.

For further questions regarding our kits contact Tecan's NGS scientific support.




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Tab 01 / DNA-Seq
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DNA-Seq solutions

  Ovation® Ultralow System V2 Celero™ DNA-Seq Mech Celero™ DNA-Seq Enz Allegro® Targeted Genotyping V2
Applications WGS
WES
Targeted sequencing
Microbial sequencing
Amplicon sequencing
WGS
WES
Targeted sequencing
Microbial sequencing
Amplicon sequencing
WGS
WES
Targeted sequencing
Microbial sequencing
Amplicon sequencing
Targeted Genotyping
Sample Type cfDNA
FFPE
Pre-fragmented gDNA
ds cDNA
FFPE
Pre-fragmented gDNA
ds cDNA
Amplicons
High quality gDNA
Large DNA fragments
High quality gDNA
Input Amount 10 pg – 100 ng 10 ng – 500 ng 10 ng – 500 ng 10 ng – 100 ng
Enzymatic Fragmentation Yes Yes
Workflow Time 4 hrs 2.5-3 hrs 2.5-3 hrs 6 hrs over 1.5 days
Index Adaptors (barcode length, number of barcodes) Single Index (8 nt, up to 96)
Unique Dual Index (UDI), (8 nt, up to 96)
UDI (10 nt, up to 384) UDI (10 nt, up to 384) Single Index (8 nt, up to 192)
Combinatorial Dual Index (when
used with Metaplex module, 8 nt, up to 3072)
UMI (Unique Molecular Identifiers) Yes, with UDI adaptors
Automation Readiness DreamPrep® NGS (Fluent® 780 workstation), and other platforms
Sequencing platform Illumina®
 
 
Tecan Patented Technologies:
NuQuant®    
DimerFree®
SPET™ (Single Primer Enrichment Technology)      

PSG_DNA-SeqSolutions_401101

 




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Tab 02 / RNA-Seq
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RNA-Seq solutions

Standard input library preparation solutions for Illumina sequencing.

  Revelo™ High Sensitivity RNA-Seq Universal Plus™ mRNA-Seq Universal Plus™ Total RNA-Seq Ovation® RNA-Seq V2 SoLo® RNA-Seq
Applications Whole transcriptome profiling
Differential gene expression
Rare transcript detection
Infectious disease
mRNA Seq
Splice variant analysis
Differential gene expression
Fusion detection
Whole transcriptome profiling
Differential gene expression
Transcript discovery
Splice variant and isoform detection
cDNA preparation from total RNA Whole transcriptome profiling
Differential gene expression
Transcript discovery
Splice variant and isoform detection
Sample Type Total RNA
FFPE
Nasal swabs
Microbial RNA
Total RNA (polyadenylated) Total RNA  (any organism) Total RNA
FFPE
Nasal swabs
Microbial RNA
Total RNA
FFPE RNA
cfRNA (liquid biopsies)
Single cell RNA
Exosome RNA
Microbial RNA
Input Amount 250 pg - 10 ng 10 ng - 1 µg 10 ng - 500 ng 500 pg - 100 ng 10 pg - 10 ng
Integrated Fragmentation Yes Yes Yes Yes
Workflow Time 6.5 hrs 7.5-8.5 hrs 7.5 hrs 4.5 hrs 14.75 hrs
Index Adaptors (barcode length, number of barcodes) Unique Dual Index (UDI) (8 nt, up to 96) UDI
(8 nt, up to 384)
UDI
(8 nt, up to 384)
N/A Single index
(8 nt, up to 96)
UMI (Unique Molecular Identifiers) Yes Yes N/A Yes
Strand Specific Yes Yes N/A Yes
Automation Readiness DreamPrep® NGS (Fluent® 780 workstation), and other platforms
Sequencing Platform Illumina® Any Illumina®
 
Tecan Patented Technologies:
NuQuant® N/A
DimerFree® N/A
SPIA® (Single Primer Isothermal Amplification)
Transcript Depletion Technology SPIABoost™ AnyDeplete® AnyDeplete N/A AnyDeplete

PSG_RNA-SeqSolutions_401104




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Tab 03 / Methyl-Seq
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Methyl-Seq solutions

  Ovation® RRBS Methyl-Seq with TrueMethyl® oxBS UltralowMethyl Seq with TrueMethyl® OXBS TrueMethyl® oxBS Module
Applications Reduced Representation Bisulfite Sequencing Methyl-Seq
Bisulfite sequencing
Oxidative bisulfite sequencing
Bisulfite conversion
Oxidative bisulfite conversion
Methyl-Seq
DNA methylation arrays
Sample Type High quality gDNA Pre-fragmented or degraded DNA
FFPE
Pre-fragmented or degraded DNA
FFPE
Input Amount 100 ng 10 ng - 300 ng 100 ng - 1 µg
Enzymatic Fragmentation Yes
Workflow Time 9 hrs 9.25 hrs 2.25 hrs
Index Adaptor (barcode length, number of barcodes) Single Index
(8 nt, up to 96)
Single Index
(8 nt, up to 96)
N/A
Diversity adaptors Yes N/A
UMI
(Unique Molecular Identifiers)
Yes N/A
Oxidative Bisulfite Reagents Included Yes (5mC and 5hmC differentiation) Yes (5mC and 5hmC differentiation) Yes (5mC and 5hmC differentiation)
Automation Readiness Beckman Coulter® Biomek i7
Agilent® Bravo®
Sequencing platform Illumina® Any

PSG_Methyl-SeqSolutions_401098

 




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Tab 04 / Microarrays
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Microarray Solutions

  Input Amount and Quality
500 pg - 50 ng
High quality or degraded RNA
50 - 100 ng
FFPE or degraded RNA
Platform Recommended Kit
Affymetrix GeneChip 3' Arrays Ovation® Pico WTA System V2 with Encore™ Biotin Module Ovation® FFPE WTA System with Encore™ Biotin Module
Affymetrix GeneChip Exon/ Gene ST Array Ovation® Pico WTA System V2 with Encore™ Biotin Module Ovation® FFPE WTA System with Encore™ Biotin Module
Agilent® Dual-Mode Gene Expression Arrays Ovation® Pico WTA System V2 Ovation® FFPE WTA System*

* May require optimization of fragment sizes

PSG_MicroarraySolutions_401873