What methods do you recommend for DNA isolation?
We recommend a column-based extraction method, including: Qiagen QIAprep Miniprep or DNeasy Miniprep kits, Zymo Quick-DNA kits and Thermo Fisher PureLink Genomic DNA kits.
Can I use phenol-chloroform based extractions for DNA isolation?
We do not recommend the use of these methods as any carryover of organics may inhibit downstream enzyme activity. If using, we recommend using a column-based purification of the downstream enzyme activity. If using, we recommend using a column-based purification of the DNA prior to input into the kit.
Can I use Celero EZ DNA-Seq with DNA from any organism?
Celero EZ DNA-Seq has been designed for use with a broad range of different organisms. Special consideration should be given when using low-input samples from organisms with large genomes.
Do I need to use high-quality DNA?
This kit is designed for use with DNA samples of high molecular weight with little or no evidence of degradation. We strongly recommend using high quality DNA with a A260:A280 ratio in excess of 1.8 and A260:A230 ratio in excess of 2.0. Use of DNA samples with lower ratios may result in low library yield.