What methods do you recommend for DNA isolation?
We recommend a column-based extraction method, including: Qiagen QIAprep Miniprep or DNeasy Miniprep kits, Zymo Quick-DNA kits and Thermo Fisher PureLink Genomic DNA kits.
Can I use phenol-chloroform based extractions for DNA isolation?
We do not recommend the use of these methods as any carryover of organics may inhibit downstream enzyme activity. If using, we recommend using a column-based purification of the downstream enzyme activity. If using, we recommend using a column-based purification of the DNA prior to input into the kit.
Can I use Celero DNA-Seq with DNA from any organism?
Celero DNA-Seq has been designed for use with a broad range of different organisms. Special consideration should be given when using low-input samples from organisms with large genomes.
Do I need to use high-quality DNA?
We strongly recommend using high quality DNA with the A260:A280 ratio in excess of 1.8. Use of DNA samples with lower ratios may result in low library yield. This kit is designed for use with DNA samples of high molecular weight with little or no evidence of degradation. FFPE DNA may be compatible with the PCR workflow. When using degraded samples, we recommend using inputs of 200 ng or greater. While it is impossible to guarantee satisfactory results with all degraded samples, this system may work with many samples that are moderately degraded.