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    Automated NGS library preparation

    NGS library preparation, quality control and pooling

    From just a few samples a week to hundreds a day, automation of NGS sample preparation allows you to reproducibly create high quality libraries for reliable next-generation sequencing (NGS).

    Free Download: ONT's Ligation Sequencing Kit XL V14 automated on the DreamPrep NGS workstation

    Free Download:

    ONT's Ligation Sequencing Kit XL V14 automated on the DreamPrep® NGS workstation
    Overview
    Get a quote Talk to an expert
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    Tab 01 / Overview
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    Our NGS library prep solutions are designed to enable your needs and accelerate your science.

    Leave library prep to us, save time for what matters more.

    Validation and verification-ready Tecan’s reagents and 3rd party’s kits to develop automated NGS library prep protocols to ensure excellent performance in any lab.

    Reliable and reproducible NGS library prep

    From plug-and-play to high to highly flexible automated solutions. Now you can walk-away with confidence.

    All in one solution with proven technology and expertise.

    From reagents, automation, and consumables to application expertise – we support you every step of the way.

    Product Features

    From low to high throughput, turnkey solutions to OEM development, our comprehensive NGS library prep solutions allow scientists to save time on library prep and focus on what matters most.

    Tecan’s Reagents​
      

    • • Applicable for a broad range of sample types
    • • Inputs as low as 10 pg for DNA-Seq and RNA-Seq

    Discover more:

    Solution Brochure

    MagicPrep® NGS​
      

    • • 8 samples per run
    • • Plug & Play
    • • Used with Tecan’s NGS reagents (DNA and mRNA-seq)

    Discover more:

    Solution Brochure

    DreamPrep® NGS Compact​

    • • Up to 48 samples per run
    • • Benchtop solution
    • • Open platform compatible with several NGS protocols

    Discover more:

    Solution Brochure

    DreamPrep® NGS​
      

    • • Up to 96 samples per run
    • • High capacity
    • • Open platform compatible with several NGS protocols

    Discover more:

    Solution Brochure

    Custom Systems for OEM Partners​

    • • Designed to your requirements and your regulatory needs
    • • Optimized for reagent kits of OEM Partner

    Discover more:

    Here

    Collaborating to Advance Genomics

    We continually strive to offer innovative, accessible and always reliable solutions.

    NGS_Collaborations_v01_1140x316

     




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    Tab 02 / DreamPrep® NGS Solutions
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    DreamPrep NGS and DreamPrep NGS Compact

    Walkaway automation solutions for NGS library prep

    Flexible configurations to deliver consistent, sequencing-ready libraries for a wide range of NGS protocols with minimal manual intervention that match your different throughput requirements.

    Watch the free webinar!

    Both solutions capitalize on the state-of-the-art Fluent® automation workstation, delivering extraordinary precision, unmatched efficiency, and seamless integration across diverse throughputs.

    DreamPrep NGS

    DreamPrep™ NGS

    • High throughputs (up to 384 samples/day1)
    • 52 deck positions
    • Plate reader and ODTC are included for long walk-away times and full NGS workflows

    Output

    • 96 DNA libraries per run in less than 4h with Tecan NGS kit Celero™
    • 96 RNA libraries per run in 9h with Tecan NGS kit Universal Plus™ mRNA
    • Targeted DNA genotyping
      • 384 samples per day with Tecan NGS kit Allegro®
      • 384 samples per day with Tecan NGS Protocol combining Tecan NGS kit Celero and QIAseq SARS-CoV-2 Primer Panel

    Software packages included

    DreamPrep NGS Compact

    DreamPrep™ NGS Compact

    • Low-medium throughputs (8-48 samples/day1)
    • Smaller footprint, 30 deck positions
    • Three flexible configurations to cover distinct customer requirements

    Three configurations are available and upgradable at any time

    Basic: Increase your productivity as all liquid transfers and incubations are covered by the platform

    Mid: Extend your walk-away time and perform amplification steps by simply adding the ODTC

    Full: Integrate Quantification and Normalizations steps in your NGS workflows by including the Infinite® 200 Pro Reader F Nano+

    Software packages included

    Revolutionize your workflows effortlessly and embrace a new era of liquid handling excellence with our NGS solutions:

    Open and flexible platform

    All components2 provide high performance across different library preparation kits

     

    Fast QC and normalization without sample loss

    Enabled by the integrated plate reader and NuQuant®3 technology

    Optimal performance with Tecan NGS reagents

    For faster and more efficient workflows

     

    Fast processing speed

    Parallel movement of robotic arms increases productivity

     

     

     

    Intuitive interaction with a touchscreen

    User-friendly interface empowers your daily operations

     

    Walk-away time solutions

    On-deck thermal cycler enables longer walk-away times

     

     

    Scale up your NGS workflows with DreamPrep NGS solutions

    These platforms combine the advanced technology of the Fluent® automation workstation, Infinite® 200 Pro Reader F Nano+ and Tecan NGS library preparation kits to generate sequencing-ready NGS libraries in one simple automated workflow.

    MagicPrep NGS
    NGS library prep at the press of a button

    Looking for lower throughputs? MagicPrep NGS is a new instrument that provides push button simplicity for NGS library preparation compatible with Illumina sequencing platforms.

    Perform robust QC in a matter of minutes, not hours

    Tecan’s innovative reagent core technology NuQuant3 and the integrated Infinite reader enable you to simultaneously measure your final libraries on a plate without unnecessary sample loss.

    DreamPrep NGS is simply fast and precise

    Save reagents and samples

    One shaker and three thermal devices for optimal library preparation

    Oxford Nanopore's Ligation Sequencing Kit XL V14 automated on the DreamPrep NGS workstation

    DreamPrep NGS has been at the forefront in viral surveillance

    Enhanced reliability with Tecan consumables

    Prevent unauthorized changes to validated protocols with the Method Approval function

    1) Number of samples processed in walk-away mode is determined by the NGS protocol

    2) Ring magnet plates and POGO® adapter with Spring Cushion Technology manufactured by ALPAQUA® Engineering, LLC. Beverly, USA.
    2) Thermal devices (Cold Plate Liquid-Cooled, CPLC) and ODTC® manufactured by Inheco© Industrial Heating and Cooling GmbH. Martinsried, Germany.
    2) BioShake®3000-T elm manufactured by QInstruments® GmbH. Jena, Germany.
    2) Infinite® 200 PRO F Nano+ Reader is a product of Tecan Trading AG, Switzerland.
    3) NuQuant® is a registered trademark of Tecan Group Ltd., Männedorf, Switzerland or of Tecan Genomics, Inc., Redwood City, USA.

    Example methods have not been validated. Please make a copy of these methods to customize them to accommodate and validate your workflow according to your desired intended use and laboratory protocols. Tecan makes no claims regarding the performance of the example methods.




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    Tab 03 / Reagents
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    Tecan NGS library prep reagents for Illumina sequencing

    Tecan has developed and optimized its own NGS library preparation reagents for a broad range of sample types. These reagents have been verified on Tecan automation platforms to give you a complete solution, from samples to readily normalized libraries.

    Integrated

    Qualified

    Illumina qualified protocols

    Tecan has collaborated with key research centers and Illumina to develop automated NGS sample preparation protocols that have been optimized, tested and sequencing verified, to ensure excellent performance in any laboratory.

    TruSeq Nano DNA

    The TruSeq Nano DNA Sample Preparation Kit enables efficient investigation of samples where the amount of DNA available is limited. The automated workflow for this kit on the Freedom EVO® NGS workstation includes simple bead-based size selection, and can be completed in less than a day with minimal hands-on time for up to 48 samples.

    Download Application Note

    TruSeq PCR Free

    The TruSeq DNA PCR-Free Sample Preparation Kit enables time-saving investigation of DNA samples. The user-friendly TouchTools™ interface guides the operator through automation set-up to deliver highly reproducible, sequencing-ready DNA libraries for single read, paired-end and indexed sequencing with minimal user intervention.

    Download Application Note

    Nextera XT

    The Nextera XT DNA Library Preparation Kit enables the effective construction of high quality libraries from PCR amplicons, plasmids and small genomes, using a protocol optimized for DNA inputs from as little as 1 ng.

    Download Application Note

    TruSeq Stranded mRNA

    RNA sequencing (RNA-Seq) is a powerful method for discovering, profiling and quantifying RNA transcripts. Using Illumina next generation sequencing technology, stranded information identifies the DNA strand from which a specific RNA transcript was derived, providing increased confidence in transcript annotation and visibility into antisense regulation.

    Download Application Note

    TruSeq Stranded Total RNA

    Automation of the Illumina TruSeq Stranded Total RNA protocol on the Freedom EVO NGS workstation enables the conversion of total RNA into libraries of template molecules of known strand origin with minimal user intervention. The Freedom EVO NGS workstation delivers high quality libraries, with minimal risk of cross-contamination.

    Download Application Note

    TruSeq RNA Access

    The TruSeq RNA Access library preparation protocol is the ideal solution for cost-effective sequencing of formalin-fixed paraffin-embedded (FFPE) archival tissue samples. The integrated enrichment steps can accommodate low starting concentrations of DNA, and the automated workflow allows the construction of up to 48 libraries in parallel, generating high quality libraries with limited user intervention and minimal risk of cross-contamination.

    Download Application Note

    Nextera Rapid Capture

    The automation-friendly workflow of the Nextera Rapid Capture protocol combined with the Freedom EVO NGS workstation provides a faster, more efficient solution for library preparation and exome enrichment. Using the automated Nextera Rapid Capture Exome protocol, sample preparation can be completed with minimal hands-on time in just a day and a half, generating sequencing data results the following day.

    Download Application Note

    TruSight® HLA v2.0 Sequencing Panel

    Interest in HLA gene sequencing is growing, as it offers better determination of the HLA profile of tissue donors and recipients compared to classic techniques, such as serotypical analysis.

    The Illumina TruSight HLA v2.0 Sequencing Panel is a reliable and simple assay designed to generate NGS libraries for HLA profiling. Automation of the workflow on the Freedom EVO NGS workstation allows sequencing of 11 HLA loci for up to 24 samples, minimizing the risk of cross-contamination and human errors during library preparation.

    Download Application Note

    TruSeq ® Custom Amplicon Low Input

    Amplicon sequencing is a targeted method for identification and analysis of variations in specific regions of the genome. Sequencing of PCR products offers cost-effective variant identification and characterization. Automation of the TruSeq Custom Amplicon Low Input library preparation workflow on the Freedom EVO NGS workstation provides a fast and efficient solution. This setup enables generation of high quality libraries from low input samples – starting with as little as 10 ng of genomic DNA.

    Verified for Illumina sequencing

    Tecan has developed, tested and sequencing-verified NGS sample preparation protocols in collaboration with NGS sample preparation kit vendors, to give you time to focus on analyzing sequencing results.

    Verified

    NEBNext Ultra II Directional RNA

    The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina uses the ‘dUTP` method to produce excellent yields and high quality directional libraries even from low quality RNA. Up to 96 libraries can be prepared at a time from a broad range of input material, 10 ng - 1 µg total RNA for poly(A) mRNA enrichment, 5 ng - 1 µg for rRNA depletion.

    Download Application Note

    NEBNext Ultra II DNA

    The NEBNext Ultra II DNA library prep workflow combines the end repair and dA-tailing steps with minimal clean-¬up to reduce sample preparation time. Up to 96 libraries can be prepared per run, and multiplexed using, for example, the NEBNext Multiplex Oligos for Illumina.

    This protocol allows highly reproducible library preparation from a wide range of input DNA concentrations from 500 pg to 1 μg, as well as FFPE samples, offering flexible processing of 1 to 96 samples with minimal user intervention.

    Download Application Note

    SureSelect XT Target Enrichment

    Agilent’s SureSelect XT Target Enrichment System provides a complete portfolio of exome kits for humans and other species, as well as custom kits for both DNA and RNA capture.

    Click here for an application note describing an automated protocol for parallel processing of up to 48 samples using the SureSelect XT Target Enrichment System on the Freedom EVO NGS workstation.

    Download Application Note

    NimbleGen SeqCap EZ Library

    Roche NimbleGen’s SeqCap EZ Library is a solution-based capture system enabling enrichment of the whole exome or custom regions of interest from DNA sequencing libraries. The SeqCap EZ protocol covers exome designs, gene panels and custom content designs, according to Roche NimbleGen specifications.

    Click here for an application note describing an automated protocol for NimbleGen SeqCap EZ with KAPA™ HTP Library Preparation in combination with NimbleGen SeqCap Adapter Kits A and/or B. The protocol allows preparation of up to 96 libraries, and capture of up to 24 library pools.

    Download Application Note

    Co-developed

    IonTorrent sequencing

    Sequencing-verified, flexible protocols for Ion AmpliSeq™ library preparation, developed in collaboration with Thermo Fisher Scientific.

    Ion AmpliSeq libraries and panels

    The Ion AmpliSeq Library Kit 2.0 enables rapid NGS library preparation for 12-25,000-plex PCR in a single well.

    Automated library preparation of up to 96 samples can be performed on the Freedom EVO NGS workstation in less than six hours using 10 ng of DNA or RNA. The TouchTools operator interface minimizes hands-on time and training requirements, guiding users through every stage of protocol selection and worktable set-up.

    Download Application Note




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    Tab 04 / Qualified NGS protocols
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    Please read the method definitions in the bottom section.

    		 NGS_Dreamprep-Fluent_300x300 DreamPrep NGS Compact NGS_Dreamprep-FreedomEvo_300x300
    Supplier Kit DreamPrep® NGS DreamPrep® NGS Compact Freedom EVO® NGS workstation
    Tecan
    DNA-Seq Celero™ DNA-Seq T      
      Celero™ EZ DNA-Seq T     T  
      Ovation® Ultralow System V2 T      
    RNA-Seq Revelo™ RNA-Seq T     T  
      Universal Plus Total RNA-Seq T      
      Universal Plus™ mRNA-Seq T     T  
    Targeted enrichment Celero™ EZ DNA-Seq and QIAseq SARSCoV- 2 Primer Panel for SARS-CoV-2 sequencing T      
    Targeted genotyping Allegro® Targeted Genotyping V2 T     T  
     
    Agilent
    Targeted enrichment Haloplex® HS Target Enrichment System      
      SureSelect XT HS2 DNA      
      SureSelectXT Target Enrichment     T  
      SureSelect® QXT Target Enrichment      
     
    Bioo Scientific
    RNA-Seq NEXTflex® Small RNA-Seq Kit v3      
     
    GenDx
    HLA typing NGSgo® HLA typing     V  
     
    Illumina
    DNA-Seq Illumina DNA PCR-Free Prep V      
      Illumina DNA Prep V     T  
      Nextera XT DNA Library Preparation Kit T     V  
      TruSeq DNA PCR-Free     V  
      TruSeq® Nano DNA     V  
    Forensics ForenSeq® DNA Signature Prep      
    HLA typing TruSight HLA v2 Sequencing Panel     V  
    RNA-Seq COVIDSeq Test (1 plate per run) V
    		   
      COVIDSeq Test (2 plates per run) V
    		   
      Illumina Stranded mRNA Prep      
      Illumina Stranded Total RNA Prep with Ribo-Zero Plus      
      TruSeq RNA Exome     V  
      TruSeq Stranded mRNA     V  
      TruSeq Stranded Total RNA Library Prep (Human/Mouse/Rat)     V  
    Targeted enrichment 16S Metagenomic Sequencing     T  
      Illumina DNA Prep with Enrichment      
      Illumina DNA Prep with Exome 2.0 Plus Enrichment      
      Illumina RNA Prep with Enrichment (L) Tagmentation      
      Nextera DNA Exome      
      TruSight Cardio      
      TruSight Inherited Disease Sequencing Panel      
      TruSight Oncology 500 DNA/RNA High-Throughput Kit      
      TruSight Oncology 500 DNA/RNA Kit      
      TruSight Oncology 500 DNA/RNA with HRD      
      TruSight Rapid Capture      
      TruSight® Cancer      
     
    Integrated DNA Technologies (IDT)
    DNA-Seq Lotus™ DNA Library Prep Kit      
      xGen™ cfDNA & FFPE DNA Library Preparation Kit      
      xGen™ NGS Hybridization Capture      
     
    Lexogen
    RNA-Seq QuantSeq 3’ mRNA-Seq V2 Library Prep Kit with UDI      
     
    MGI
    DNA-Seq MGIEasy FS DNA Library Prep      
    RNA-Seq MGIEasy RNA Directional Library Prep Set      
     
    New England BioLabs (NEB)
    DNA-Seq NEBNext® Ultra™ II DNA     V  
    RNA-Seq NEBNext Ultra II Directional RNA Poly(A) mRNA Isolation     V  
      NEBNext Ultra II Directional RNA rRNA depletion     V  
     
    Oxford Nanopore Technologies (ONT)
    DNA-Seq Ligation Sequencing Kit XL V14 (SQK-LSK114-XL) V      
      Native Barcoding Kit 96 V14 (SQK-NBD114.96) V      
    RNA-Seq Midnight kit with ARTIC method for high-throughput SARS-CoV-2 sequencing      
     
    Pacific Biosciences (PacBio)
    DNA-Seq SMRTbell prep kit 3.0   V    
     
    QIAGEN
    DNA-Seq QIAseq® FX DNA      
    RNA-Seq QIAseq Targeted RNA Panels      
    Targeted enrichment QIAseq DIRECT SARS-CoV-2      
      QIAseq Targeted DNA Panels      
     
    Quantabio
    DNA-Seq sparQ DNA Library Prep Kit      
     
    Roche
    DNA-Seq KAPA HyperPrep      
      KAPA® HyperPlus      
    RNA-Seq KAPA RNA HyperPrep with RiboErase (HMR)     T  
      RNA HyperPrep with mRNA Capture     T  
     
    Thermo Fisher Scientific
    DNA-Seq Ion Xpress™      
    RNA-Seq Oncomine™ Comprehensive Assay v3M      
    Targeted enrichment Ion AmpliSeq® Library 2.0     V  
     
    Twist
    DNA-Seq Library Preparation EF 2.0 and Twist Universal Adapter System      
    Targeted enrichment Human Core Exome      
      Twist Target Enrichment Standard Hybridization v2      
     

    Method available:

    Automated method developed according to the kit vendor’s published protocol. Protocol was tested using water and/or reagents with Tecan instruments.

     

    Method qualification in progress:

    Automated method developed according to the kit vendor’s published protocol. It is currently being tested with reagents and commercial samples by the vendor.

     

    Method qualified: V T C

    Automated method developed according to the kit vendor’s published protocol. Method was qualified using kit vendor’s guidelines, reagents and commercial samples. Libraries prepared with these methods meet the kit vendor’s specifications and/or perform comparably to those prepared manually. Sequenced libraries were assessed either by the vendor (V vendor qualified), by Tecan (T Tecan qualified) or by the customer (C customer qualified).

     

    Method custom developed:

    Automated method developed according to specific customer need. Libraries tested by the customer.




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    Tab 05 / Freedom® Evo NGS workstation
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    There are so many things that you have to get right to create high quality libraries for reliable next generation sequencing, so why have unnecessary complexity in your liquid handling?

    Automation of NGS sample preparation on the Freedom EVO NGS workstation increases throughput and reproducibility, guiding the user through each step of protocol selection and worktable set-up with the TouchTools™ operator interface.

    Freedom EVO NGS workstation

    The Freedom EVO NGS workstation is equipped with innovative air displacement pipetting technology, using eight pipetting channels (1) to allow precise pipetting from 1,000 µl down to 0.5 µl. The workstation includes three Inheco CPAC devices (2) to keep reagents cool and provide optimal conditions for the enzymatic steps, an Inheco Thermoshake heated shaker (3), a 96-position Alpaqua 96S Super Magnet Plate magnetic plate separator (4) and a RoMa Arm (5) for efficient bead clean-up. In addition, the compact worktable provides storage space for up to 12 tip boxes (6), allowing longer unattended runs.

    Precision: The Freedom EVO’s precision automation ensures the reproducibility necessary for high quality results, with a range of qualified sequencing-verified protocols available.
    Flexibility: Don’t be constrained by batching requirements. Whether you need to process 6, 13, 19, 40 or even 96 samples, only run what you need, saving on reagents.
    Simplicity: You don’t need to be an automation expert. With the simple touchscreen interface, you can get started right away, set up your runs quickly and be confident in your workflows.

    NGS Sample preparation Freedom EVO with Touchtools

    TouchTools operator interface

    The TouchTools application starter screen lets the operator select the desired protocol with the touch of a button. Step-by-step user instructions help to avoid potential errors during worktable set-up to ensure the correct initial conditions. A selection confirmation screen then provides a summary as a final check before starting the run, making it easy for anyone in the lab to use the instrument.

    Installation and support

    The Freedom EVO NGS workstation is supplied with one of the standard protocols available at the time of installation, including water testing and user training. Additional protocols can be installed at any time.

    Tecan Journal Articles

    Off-the-shelf NGS library prep for Ion Torrent™ sequencing
    [TJ 03/2015]

    The Leiden University Medical Center has integrated the Freedom EVO® NGS workstation into its molecular diagnostic testing and clinical research workflows. Taking advantage of this preconfigured solution, the center was quickly able to commission the platform for routine testing, while still having the flexibility to develop new protocols and conduct research studies.

    Enhancing NGS sample prep with automation
    [TJ 02/2014]

    The University of North Carolina (UNC) has automated its major sample preparation protocols for next generation sequencing on a Freedom EVO® workstation, enabling more efficient processing.

    GeT with the NGS program
    [TJ 02/2014]

    The GeT genomics facility near Toulouse has been developing a series of automated NGS protocols in collaboration with Tecan – including Illumina’s TruSeq® DNA and RNA kits, as well as Bioo Scientific’s NEXTflex™ PCR-Free Modules – on two Freedom EVO® workstations, helping to improve throughput and reliability for a wide range of projects.




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    Tab 06 / Detection
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    Sensitive and fast quantification

    The Infinite® F Nano+ multimode reader offers absorbance and high sensitivity fluorescence reading modes in one compact instrument. It combines the high sensitivity of fluorescence assays and the speed provided by reading plate formats of up to 384 wells.

     

    NGS library quantification is a crucial step for a successful sequencing run. Accurately determining the number of sequenceable molecules is highly significant for new high capacity sequencers, such as the NovaSeq system.

    Tecan NuQuant technology

    Tecan NuQuant technology

    Tecan NuQuant® is a novel library quantification method that accurately measures molar library concentration without the need for separate fragment size analysis. This proprietary method uses a fluorescent label that is incorporated into the library molecules during library preparation. NuQuant is independent of library size, allowing library molarity to be directly measured using fluorometers, such as the Infinite F Nano+ multimode reader.

    Integrated library QC in less than six minutes

    NuQuant library quantification on the Infinite F Nano+ plate reader eliminates the need for additional manual qPCR or capillary electrophoresis steps, saving significant time, resources and valuable library material.

    After final purification of the DNA libraries in a 96-well plate on the Fluent ® Automation Workstation, the fluorescence of the libraries and standards is measured on the Infinite plate reader. The molarity determined for each library can then be used for balanced pooling and subsequent sequencing on Illumina instruments.

    Other quantification assays

    Other quantification assays

    Reader integration for quantification

    DNA quantification can be achieved by measuring an aliquot with a fluorescent dye, such as PicoGreen® or QuantiFluor™, or by measuring the absorbance of the entire DNA sample. DNA quantification and normalization workflows typically include the following steps

    • Set-up of quantification assay
    • Determination of the DNA concentration in a reader
    • Calculation of a dilution scheme to achieve the target concentration for each well
    • Dilution of the DNA samples to the desired concentration.




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    Tab 07 / Literature
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