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Product selection guide

Find the right product for your experiment.

For further questions regarding our kits contact Tecan's NGS scientific support.

DNA-Seq solutions

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	Ovation® Ultralow System V2	Celero™ DNA-Seq Mech	Celero™ DNA-Seq Enz	Allegro® Targeted Genotyping V2
Applications	• WGS • WES • Targeted sequencing • Microbial sequencing • Amplicon sequencing	• WGS • WES • Targeted sequencing • Microbial sequencing • Amplicon sequencing	• WGS • WES • Targeted sequencing • Microbial sequencing • Amplicon sequencing	• Targeted Genotyping
Sample Type	• cfDNA • FFPE • Pre-fragmented gDNA • ds cDNA	• FFPE • Pre-fragmented gDNA • ds cDNA • Amplicons	• High quality gDNA • Large DNA fragments	• High quality gDNA
Input Amount	10 pg – 100 ng	10 ng – 500 ng	10 ng – 500 ng	10 ng – 100 ng
Enzymatic Fragmentation	—	—	Yes	Yes
Workflow Time	4 hrs	2.5-3 hrs	2.5-3 hrs	6 hrs over 1.5 days
Index Adaptors (barcode length, number of barcodes)	• Single Index (8 nt, up to 96) • Unique Dual Index (UDI), (8 nt, up to 96)	• UDI (10 nt, up to 384)	• UDI (10 nt, up to 384)	• Single Index (8 nt, up to 192) • Combinatorial Dual Index (when used with Metaplex module, 8 nt, up to 3072)
UMI (Unique Molecular Identifiers)	Yes, with UDI adaptors	—	—	—
Automation Readiness	DreamPrep® NGS (Fluent® 780 workstation), and other platforms
Sequencing platform	Illumina®


Tecan Patented Technologies:
NuQuant®		✓	✓
DimerFree®	✓	✓	✓	✓
SPET™ (Single Primer Enrichment Technology)				✓

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RNA-Seq solutions

Standard input library preparation solutions for Illumina sequencing.

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	Revelo™ High Sensitivity RNA-Seq	Universal Plus™ mRNA-Seq	Universal Plus™ Total RNA-Seq	Ovation® RNA-Seq V2	SoLo® RNA-Seq
Applications	• Whole transcriptome profiling • Differential gene expression • Rare transcript detection • Infectious disease	• mRNA Seq • Splice variant analysis • Differential gene expression • Fusion detection	• Whole transcriptome profiling • Differential gene expression • Transcript discovery • Splice variant and isoform detection	• cDNA preparation from total RNA	• Whole transcriptome profiling • Differential gene expression • Transcript discovery • Splice variant and isoform detection
Sample Type	• Total RNA • FFPE • Nasal swabs • Microbial RNA	• Total RNA (polyadenylated)	• Total RNA (any organism)	• Total RNA • FFPE • Nasal swabs • Microbial RNA	• Total RNA • FFPE RNA • cfRNA (liquid biopsies) • Single cell RNA • Exosome RNA • Microbial RNA
Input Amount	250 pg - 10 ng	10 ng - 1 µg	10 ng - 500 ng	500 pg - 100 ng	10 pg - 10 ng
Integrated Fragmentation	Yes	Yes	Yes	—	Yes
Workflow Time	6.5 hrs	7.5-8.5 hrs	7.5 hrs	4.5 hrs	14.75 hrs
Index Adaptors (barcode length, number of barcodes)	Unique Dual Index (UDI) (8 nt, up to 96)	UDI (8 nt, up to 384)	UDI (8 nt, up to 384)	N/A	Single index (8 nt, up to 96)
UMI (Unique Molecular Identifiers)	—	Yes	Yes	N/A	Yes
Strand Specific	—	Yes	Yes	N/A	Yes
Automation Readiness	DreamPrep® NGS (Fluent® 780 workstation), and other platforms				—
Sequencing Platform	Illumina®			Any	Illumina®

Tecan Patented Technologies:
NuQuant®	✓	✓	✓	N/A	—
DimerFree®	✓	✓	✓	N/A	✓
SPIA® (Single Primer Isothermal Amplification)	✓	—	—	✓	—
Transcript Depletion Technology	SPIABoost™	AnyDeplete®	AnyDeplete	N/A	AnyDeplete

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Methyl-Seq solutions

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	Ovation® RRBS Methyl-Seq with TrueMethyl® oxBS	Ultralow^™Methyl Seq with TrueMethyl® OXBS	TrueMethyl® oxBS Module
Applications	• Reduced Representation Bisulfite Sequencing	• Methyl-Seq • Bisulfite sequencing • Oxidative bisulfite sequencing	• Bisulfite conversion • Oxidative bisulfite conversion • Methyl-Seq • DNA methylation arrays
Sample Type	• High quality gDNA	• Pre-fragmented or degraded DNA • FFPE	• Pre-fragmented or degraded DNA • FFPE
Input Amount	100 ng	10 ng - 300 ng	100 ng - 1 µg
Enzymatic Fragmentation	Yes	—	—
Workflow Time	9 hrs	9.25 hrs	2.25 hrs
Index Adaptor (barcode length, number of barcodes)	Single Index (8 nt, up to 96)	Single Index (8 nt, up to 96)	N/A
Diversity adaptors	Yes	—	N/A
UMI (Unique Molecular Identifiers)	Yes	—	N/A
Oxidative Bisulfite Reagents Included	Yes (5mC and 5hmC differentiation)	Yes (5mC and 5hmC differentiation)	Yes (5mC and 5hmC differentiation)
Automation Readiness	• Beckman Coulter® Biomek i7 • Agilent® Bravo®		—
Sequencing platform	Illumina®		Any

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Microarray Solutions

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	Input Amount and Quality
	500 pg - 50 ng High quality or degraded RNA	50 - 100 ng FFPE or degraded RNA
Platform	Recommended Kit
Affymetrix GeneChip 3' Arrays	Ovation® Pico WTA System V2 with Encore™ Biotin Module	Ovation® FFPE WTA System with Encore™ Biotin Module
Affymetrix GeneChip Exon/ Gene ST Array	Ovation® Pico WTA System V2 with Encore™ Biotin Module	Ovation® FFPE WTA System with Encore™ Biotin Module
Agilent® Dual-Mode Gene Expression Arrays	Ovation® Pico WTA System V2	Ovation® FFPE WTA System*

* May require optimization of fragment sizes

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