How should I QC my cDNA for use with the Encore Biotin Module?
You must use cDNA generated with a validated Tecan amplification system. The concentration of starting cDNA must be determined to ensure adequate input into the labeling reaction and, therefore, onto the arrays. Please refer to Table 2 in the User Guide for cDNA input requirements
What type of cDNA should I use with the Encore Biotin Module?
You must use SPIA cDNA generated with one of the following Tecan products with the Encore Biotin Module:
- Ovation RNA Amplification System V2 (Part No. 3100)
- Ovation Whole Blood Solution (Part Nos. 1300 and 3100)
- Ovation Pico WTA System V2 (Part No. 3302)
- Ovation PicoSL WTA System V2 (Part No. 3312)
- Ovation FFPE WTA System (Part No. 3403)
Can I vary the amount of cDNA input used for fragmentation and labeling?
You may use from 2 to 6 μg of cDNA input with the Encore Biotin Module. Refer to Table 2 in the User Guide to determine the specific recommendations for your sample type, array type and amplification kit used. We recommend using the same cDNA input across all samples in a single experiment where a range of inputs is given.
Can I use any cDNA as starting material in the Encore Biotin Module?
No, the cDNA must be generated using a compatible Tecan RNA Amplification System. Use of other cDNAs will result in poor performance.
Can the Encore Biotin Module be used for fragmentation and labeling of RNA?