Protein purification and bioprocessing

Tecan’s Freedom EVO® liquid handling platform and Fluent® Automation Workstation offer fully automated, parallel column chromatography using Repligen’s OPUS® RoboColumn® system, completely eliminating manual processing steps. Miniaturization and parallel processing help to save precious biologics and reagents, reducing costs and shortening development times for new therapeutics.

The Freedom EVO® and Fluent® platforms offers high throughput automated protein purification and bioprocessing for the development and manufacture of therapeutic agents. The miniaturization and parallelization capabilities of the platforms ensure a robust scale-up process, guaranteeing cost-and time-efficient development.

Using the OPUS® RoboColumns® on the Freedom EVO® and Fluent® platforms enables true parallel chromatography in up to eight columns, offering rapid screening of a range of parameters in the shortest time frame possible, without using sophisticated LC systems.

This application note describes the purification of harvested and clarified cell culture fluid (HCCF) samples using OPUS® RoboColumns® and the Te-Chrom™ module on the Fluent® Automation Workstation, and compares the results to a typical lab-scale purification using an ÄKTA® avant (Cytiva).

This application note describes how MSD is using RoboColumns® on the Freedom EVO® platform to qualify HTPD methods for the characterization of mAb purification steps, for both bind and-elute cation exchange (CEX) and flow-through anion exchange (AEX) studies.

Purification of biopharmaceuticals is still dominated by sequential liquid chromatography in a packed column bed, which is not only difficult and time consuming, but also very expensive. This application note describes the implementation of automated resin screening using Repligen’s OPUS RoboColumns on the Freedom EVO liquid handling platform.

Combining PhyTip columns with the Freedom EVO platform offers high throughput automation for sample preparation and protein purification.

The Freedom EVO platform offers complete automation of sample preparation and protein purification using PhyTip columns, allowing straightforward process development and predictable scale-up procedures.

The Freedom EVO workstation can be used to screen chromatographic process parameters, helping to accelerate development of biopharmaceutical purification processes. The system can be used with a wide range of modules and accessories, such as the MediaScout® RoboColumns® miniaturized chromatography columns.

Automated process development on the Freedom EVO platform can accelerate development of both upstream and downstream processes for large-scale bioproduction, as well as improving process analytics.

Automation with the Freedom EVO to accelerate cell culture upscaling, downstream process development and process analytical technology (PAT).

An overview of chromatographic process development tools on the Freedom EVO platform, including finite bath thermodynamic studies, batch binding in PreDictor™ plates, bind/elution studies, and breakthrough/elution experiments with MediaScout RoboColumns.

High throughput bioprocess development is essential for optimizing run parameters prior to scale-up. University College London’s Advanced Centre for Biochemical Engineering is harnessing the possibilities afforded by automation, using custom-built scripts and programs to make its liquid handling platforms accessible to inexperienced users.

Biomanufacturing requires careful separation of the molecule of interest from other cellular products to ensure the quality and stability of the final product. This is even more important for the manufacture of biotherapeutics, as the presence of unwanted molecules in pharmaceutical preparations can affect the efficacy and safety of biologically-derived drugs. FUJIFILM Diosynth Biotechnologies is using automated chromatography condition screening to generate more data in less time, helping to improve the performance of purification processes and accelerate biological drug manufacturing.

The biosimilars market is expanding rapidly as the patents expire for an increasing number of high profile biopharmaceutical agents. The complex nature of biologics requires extensive characterization of the production techniques and in vivo effects of new biosimilars before they can be released onto the market. Coherus Biosciences is using advanced laboratory automation to help screen chromatography conditions as part of its downstream purification processes for new biosimilars.

Merck KGaA has implemented automated miniature column chromatography protocols to improve throughput and reduce the amount of material required for the development and characterization of new chromatography media, allowing more measurements to be performed in less time.

SomaLogic's SOMAscan® proteomics technology is helping to identify new biomarkers for a wide range of disease states. Capable of simultaneously measuring thousands of proteins in low volume biological samples, this technique is ideal for the detection of low abundance proteins.

Thermo Fisher Scientific has developed innovative sample processing tools designed to simplify and accelerate analytical workflows. Offering automation-friendly affinity purification for proteins and antibodies, this provides a robust solution for high throughput preclinical research and development.

UCL’s Advanced Centre for Biochemical Engineering is harnessing the flexible automation capabilities of its Freedom EVO workstations to explore novel bioprocessing applications for the production of ‘next generation’ medicines, including protein-derived therapeutics, vaccines and cells for therapy.

Tecan and GE Healthcare are working together to increase the quality and consistency of high throughput protein purification for process development, combining the Freedom EVO workstation with PreDictor 96-well plates pre-filled with BioProcess™ chromatography media.

UCB has increased the throughput of its antibody purification pipeline by using a Freedom EVO workstation to miniaturize its chromatography applications, allowing the company to investigate far more parameters in a fraction of the time, while consuming significantly less material.

The combination of the Freedom EVO platform and Atoll’s MediaScout RoboColumns offers a powerful tool for fully automated high throughput process development, allowing valid chromatographic conditions to be used without the need for sophisticated LC systems.

Scientists at Bayer Schering Pharma’s Berlin research facility have integrated two Freedom EVO workstations into their protein production process, including automation of GE Healthcare’s MultiTrap® 96-well plates.