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Uno Single Cell Dispenser™

Meet Uno: The simplicity of single cell isolation at your fingertips

The Uno Single Cell Dispenser is an automated benchtop instrument designed for ease of use, efficiency, and precision in single-cell workflows. Uno empowers a wide range of single-cell applications across the "-omics" landscape, including MS-based single-cell analysis, iPSC libraries, 3D cell research, cell-line development, and beyond.

With Uno, you can achieve accurate, viable, and precise single-cell dispensing, managing reagent volumes from picoliters to microliters within minutes.
Overview
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Tab 01 / Overview
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We now use the Uno routinely ... it's just so easy and fast, and we know we can trust its accuracy.
Ryan Kelly, PhD, Professor, BYU Department of Chemistry and Biochemistry
Read more in "Opening The Door To Single-cell Proteomics"

Tired of spending hours on manual single cell isolation with limited success? Struggling with the shortcomings of the conventional FACS method, which leads to decreased cell viability and altered cell function post-sorting?

Looking for a simple and cost-effective solution to dispense your single cells?

Why choose Tecan's Uno Single Cell Dispenser?

Simple and accessible benchtop solution for labs of any size

Integrate intuitive software and compact hardware at a competitive price without compromising on performance.

Fast and precise dispensing saves reagents with picoliter-level accuracy

Isolate single cells in ~5 minutes, dispense reagents in <3 minutes across a 384-well plate and dispense up to 25 cells per well in one run.

Reliable dispensing process for reproducible results

Based on HP’s proven microfluidic digital dispensing technology, a choice trusted by 1,000+ industry-leading customers.

Gentle single-cell dispensing to preserve cell integrity

Maintain a robust 90% cell viability [1] for downstream analysis. Works with a wide range of cells and reagents.

[1] <1hour post-dispense

Featured Workflow

Combine cell and reagent dispensing on the same instrument.

Uno_KeyVisual_Process_HP_v01

 

Product Features

Uno Single Cell Dispenser specification

Specification

Specification/Description

Cell size range

Cell size range

9 µm to 25 µm

Cell types

Cell types

Wide variety of cell types are tested

Qualified fluid classes

Qualified fluid classes

DMSO, Acetonitrile, aqueous solutions, aqueous 10% trifluoroacetic acid (TFA), master mixes

Formats to dispense on

Formats to dispense on
  • 12, 24, 48, 96, 384 well plates
  • Customizable substrate, such as microscope slides
  • Plate height 4-47mm

Cell isolating technology

Cell isolating technology

Microfluidic technology

Dispensing speed

Dispensing speed
  • Cells: 384-well plate in ~5 minutes
  • Reagents: 384-well plate in <3 minutes

Cell dispensing

Cell dispensing
  • Choose between single cell and multi-cell dispensing
  • Dispense from 1 to 25 cells per well

Cell viability

Cell viability

90% [2]

Software

Software
  • Intuitive and easy to set-up protocols
  • Automatically generates summary report, indicating wells with single cells

Overall instrument dimensions

Overall instrument dimensions

47 cm x 38 cm x 23 cm

Instrument weight

Instrument weight

4.8 kg (10.58 lb)

[2] At day zero




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Tab 02 / Software
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The instrument is combined with the simple, yet robust UnoControl software. UnoControl allows you to intuitively and quickly setup your single cell or reagent dispense protocol. Once you hit run, the software guides you step-by-step through your protocol.

After each run, you can access immediate insights through auto-generated summary reports. These reports provide information on dispensed reagent volumes, used plates and cassettes, single-cell isolation success rate, and cell concentration.

Learn UnoControl and how to successfully run the Uno Single Cell Dispenser in no time.

UnoControl Software

Designed with simplicity in mind. 

Intuitive fluid and plate definition enables protocol set-up in minutes.

 

The protocol set-up guide includes a wide range of qualified fluid classes, covers four distinct cell size ranges and allows to dispense up to 25 cells per well.

 

Guided steps ensure precise execution of experiments, minimizing handling errors.

 

Gain instant insights with auto-generated summary reports that detail dispensed reagent volumes, plates and cassettes used, single-cell isolation success rate, and cell concentration.

 




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Tab 03 / Literature
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Brochure

Uno Single Cell Dispenser

Simplyfy your Single Cell Workflow like never before. Meet Tecan's Uno Single Cell Dispenser.

Uno Single Cell Dispenser - Specifications

Simple Guide

Culture Preparation for Dispensing Mammalian Cells

This protocol has been developed specifically for HEK-293 cells. Modifications may be necessary for other cell lines.
Related Products
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T1 & T1sf Dispensehead Cassette

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D1 & D1sf Dispensehead Cassette

For non-contact reagent dispensing from 1nl to 200µL

Downloads
Uno Single Cell Dispenser Brochure

Simplyfy your Single Cell Workflow like never before. Meet Tecan's Uno Single Cell Dispenser.


For research use only. Not for use in diagnostic procedures.
Trade name: Uno; Product name: Single Cell Dispenser; Tecan model no: 30230840
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