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    Infinite® 200 PRO Configurations

    Six configurations. Infinite possibilities.

    The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable high performance detection solutions empowered by monochromator- or filter based technologies. The six new tailored configurations provide excellence in ELISA assays, nucleic acid quantifications, reporter assay technologies, and drug discovery assays including HTRF® and fluorescence polarization. This fully upgradeable reader family is designed to simplify your target applications, making it easier than ever to choose the perfect reader for your needs and budget.

     

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    Infinite Lumi

    Plate reader with dedicated optics designed to deliver exceptional results for glow, flash and dual-color luminescence applications.

    Features and technologies

    This instrument is equipped with a dedicated luminescence module for measuring glow and dual-color luminescent assays. The addition of an optional Te-Inject™ reagent injector, offers greater versatility by allowing flash luminescence measurements.

    An integrated optical fiber delivers optimal sensitivity (typically <12 amol/well ATP), and is optimized for use with plates up to 384 wells. This makes the instrument the perfect choice for increasing sample throughput, saving your lab time and money!

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    Both channels of the reagent injector are compatible with 384-well plates, allowing you to perform Dual Luciferase Reporter® assays (Promega) in a high throughput fashion.

    The system’s luminescence filter wheel also allows you to perform dual-color luminescence assays, including the new NanoBRET™ technology. These dedicated and validated filters are pre-installed, helping to guarantee maximum performance for advanced assay technologies.

    Key applications

    • ATP quantification
    • Luminescence reporter assays 
    • BRET2
    • NanoBRET
    • ChromaGlo™
    • Dual Luciferase Reporter Assay System
    • Chemiluminescent ELISAs
    • Ca2+ release assays

    Literature

    This Technical Note describes optimized test set-ups, instrument settings and the statistical equations that were used to determine the maximum instrument sensitivity of the Infinite F200 PRO filter-based microplate reader and the Infinite M200 PRO Quad4 Monochromators-based microplate reader for flash and glow luminescence.

    The BacTiter-Glo Microbial Cell Viability Assay was tested on the Infinite F200 PRO filter-based multimode reader, using the instrument’s highly sensitive luminescence module.

    This note describes the Dual Luciferase Reporter Assay performance using Tecan’s Infinite 200 PRO multimode reader equipped with a two channel injector.

    Dynamic interactions between proteins are key mediators of multiple cellular functions and are involved in almost all biological processes. Accordingly, they are valuable targets for the development of novel drug therapies. In this context, bioluminescence resonance energy transfer (BRET), developed by Promega, is a well-established detection technology for the investigation of protein-protein interactions in living cells. However, its application has so far been somewhat hampered by its limited dynamic range and sensitivity.

    Infinite M Nano

    Upgradeable absorbance plate reader delivering sensitive results for ELISAs and low volume nucleic acid or protein quantification assays. Automated pathlength correction and low volume NanoQuant plate deliver high precision results every day, independent from sample volumes.

    Features and technologies

    The Infinite M Nano’s excitation monochromator is specifically optimized for wavelength accuracy and precision, delivering excellent performance for every absorbance assay.

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    Exceptional wavelenth accuracy (typically <0.5 % at 260 nm ) and precision (typically < 0.2 % at 260 nm ) prerequisites for high performance absorbance applications, including nucleic acid quantification and spectral recording.

    With the optional low volume NanoQuant Plate™ and cuvette port, this reader represents a perfect tool for DNA/RNA quantifications (A260/280) and purity checks (A260/230), allowing you to work with a variety of sample formats and volumes, from 2 µl to 1 ml.

    Well-to-well variations in path length can be a major issue for absorbance measurements in microplates, so i-control offers easy path length correction, giving you full control for comparable readouts every time.

    Key applications

    • DNA quantification
    • RNA quantification
    • A260/280 purity checks
    • Labeling effiency
    • Absorbance-based ELISAs
    • Protein quantification (e.g. BCA, Bradford, Lowry)
    • 600 nm growth curves (bacteria, yeast)
    • Enzyme kinetics
    • Compound characterization

    Literature

    The NanoQuant Plate from Tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. Additionally, the efficiency of the nucleic acid labeling with dyes can be easily determined with this tool.

    Siloam Biosciences’ OptiMax microplate (based on the Optimiser technology platform) offers rapid, sensitive and specific chemifluorescence-based ELISA procedures using exceedingly small sample volumes. The speed, sensitivity and small sample requirements are achieved as a result of the unique microfluidic design of the Optimiser technology.

    In this note we describe the use of Tecan’s Infinite F200 PRO and Infinite M200 PRO instruments for easy and sensitive protein quantification using different absorbance-based assays.

    This application note describes the detection of S. aureus growth using Tecan’s Infinite 200 PRO microplate reader to perform 600 nm absorbance measurements. The influence of assay parameter and instrument settings on data quality is discussed.

    The isolation of adult stem cells results in very low amounts of cells due to their rare incidence in already formed tissues and organs. Here we describe the preparation and evaluation of RNA samples from a novel population of mouse keratinocyte stem cells marked by Lgr5+ expression. We used tools specifically designed for handling and measuring low RNA quantities including RNA purification and the subsequent quantification with the Infinite M200 PRO.

    Tecan´s NanoQuant detection technology has been developed to facilitate the quantification of biomolecules in small-volume samples. The present note describes the implementation of the NanoQuant Plate for protein measurements with regard to essential assay parameters such as linearity, uniformity, and reproducibility.

    One of the most used methods for determining nucleic acid concentrations is based on measuring the optical density of a sample at 260 nm (OD260). According to the Lambert-Beer law, the amount of absorbed light is proportional to the concentration of the sample, and to the pathlength the light has to pass through when going through the sample.

    Infinite M Nano+

    Dual-mode plate reader with monochromator-based optics for absorbance and sensitive fluorescence (top and bottom reading) applications. Your adjustable tool, even for low concentration nucleic acid and protein quantification.

    Features and technologies

    The Infinite M Nano+ has an excitation monochromator optimized for wavelength accuracy and precision, ensuring excellent performance for every absorbance and fluorescence assay.

    Engineered for absorbance and fluorescence measurements, the system’s highly sensitive Quad4 Monochromators™ minimize stray light, delivering exceptional flexibility with sensitivity levels close to comparatively priced filter-based instruments.

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    This system is the perfect solution for nucleic acid quantification, especially if A260 measurements are not sensitive enough. With DNA concentrations of <1 ng/µl, accurate quantification requires a fluorescent dye, such as Quant-iT™ PicoGreen® (Thermo Fisher). The design of the instrument’s top reading fluorescence optics allow it to measure fluorescently-labeled nucleic acids in both microplates and Tecan’s low volume NanoQuant Plate™ – offering virtually the same capabilities as a NanoDrop® and a Qubit™ in one device, while still supporting microplate formats and being fully upgradeable.

    Key applications

    • Absorbance-based DNA/RNA quantification and purity checks
    • Fluorescence-based DNA/RNA quantification (PicoGreen, RiboGreen®)
    • Absorbance-based protein quantification (BCA, Bradford, Lowry, etc.)
    • Fluorescence-based protein quantification (eg. NanoOrange®)
    • Absorbance- and fluorescence-based ELISAs 600 nm growth curves (bacteria, yeast)
    • Enzyme kinetics
    • Compound characterization

    Literature

    The NanoQuant Plate from Tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. Additionally, the efficiency of the nucleic acid labeling with dyes can be easily determined with this tool.

    Siloam Biosciences’ OptiMax microplate (based on the Optimiser technology platform) offers rapid, sensitive and specific chemifluorescence-based ELISA procedures using exceedingly small sample volumes. The speed, sensitivity and small sample requirements are achieved as a result of the unique microfluidic design of the Optimiser technology.

    In this note we describe the use of Tecan’s Infinite F200 PRO and Infinite M200 PRO instruments for easy and sensitive protein quantification using different absorbance-based assays.

    The isolation of adult stem cells results in very low amounts of cells due to their rare incidence in already formed tissues and organs. Here we describe the preparation and evaluation of RNA samples from a novel population of mouse keratinocyte stem cells marked by Lgr5+ expression. We used tools specifically designed for handling and measuring low RNA quantities including RNA purification and the subsequent quantification with the Infinite M200 PRO.

    Tecan´s NanoQuant detection technology has been developed to facilitate the quantification of biomolecules in small-volume samples. The present note describes the implementation of the NanoQuant Plate for protein measurements with regard to essential assay parameters such as linearity, uniformity, and reproducibility.

    This application note describes the detection of S. aureus growth using Tecan’s Infinite 200 PRO microplate reader to perform 600 nm absorbance measurements. The influence of assay parameter and instrument settings on data quality is discussed.

    This note describes the implementation of the Infinite 200 PRO multimode reader and the associated NanoQuant Plate for fluorescence-based DNA quantification in small volume samples using Pico Green®, an ultra-sensitive fluorescent dye for the quantitation of double-stranded DNA.

    This note describes the use of the fluorescence microplate reader Tecan Infinite F200 PRO or M200 PRO, for detection of as lias 20 pg dsDNA in a 200 μl assay volume.

    This Technical Note describes the relevant instrument parameters to acquire optimal fluorescence excitation and emission spectra on the Infinite M200 PRO.

    One of the most used methods for determining nucleic acid concentrations is based on measuring the optical density of a sample at 260 nm (OD260). According to the Lambert-Beer law, the amount of absorbed light is proportional to the concentration of the sample, and to the pathlength the light has to pass through when going through the sample.

    Infinite F Nano+

    Dual-mode plate reader with filter-based optics for absorbance and fluorescence (top and bottom reading) applications. Your cost-effective tool for highest demands in sensitivity.

    Features and technologies

    The Infinite F Nano+ has been created to provide an affordable, filter-based solution delivering sensitive results for typical absorbance and fluorescence applications.

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    Using filter-based optics offers higher sensitivity than monochromator-based systems, with the trade-off being reduced flexibility in wavelength selection. The Infinite F Nano+ minimizes this inconvenience by using L-shaped filter slides, which can hold up to four absorbance or fluorescence filters. Each slide has an ID chip that allows you to save filter definitions directly onto, enabling the system to automatically recognize the filters and simplifying slide handling.

    The system’s fluorescence optics include dichroic mirrors to dramatically improve performance for fluorescent dyes that are excited below and emit light above 510 nm, such as fluorescein. This ensures very low detection limits, typically around 85 amol/well (384-well plate, 100 µl).

    Key applications

    • Absorbance-based DNA/RNA quantification and purity checks (A260/280)
    • Fluorescence-based DNA/RNA quantifications (PicoGreen®, RiboGreen®)
    • Absorbance-based protein quantification (BCA, Bradford, Lowry, etc.)
    • Absorbance and fluorescence-based ELISAs
    • 600 nm growth curves (bacteria, yeast)
    • Enzyme kinetics
    • ORAC assays
    • Time-resolved fluorescence (TRF)

    Literature

    The NanoQuant Plate from Tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. Additionally, the efficiency of the nucleic acid labeling with dyes can be easily determined with this tool.

    Siloam Biosciences’ OptiMax microplate (based on the Optimiser technology platform) offers rapid, sensitive and specific chemifluorescence-based ELISA procedures using exceedingly small sample volumes. The speed, sensitivity and small sample requirements are achieved as a result of the unique microfluidic design of the Optimiser technology.

    In this note we describe the use of Tecan’s Infinite F200 PRO and Infinite M200 PRO instruments for easy and sensitive protein quantification using different absorbance-based assays.

    The isolation of adult stem cells results in very low amounts of cells due to their rare incidence in already formed tissues and organs. Here we describe the preparation and evaluation of RNA samples from a novel population of mouse keratinocyte stem cells marked by Lgr5+ expression. We used tools specifically designed for handling and measuring low RNA quantities including RNA purification and the subsequent quantification with the Infinite M200 PRO.

    Tecan´s NanoQuant detection technology has been developed to facilitate the quantification of biomolecules in small-volume samples. The present note describes the implementation of the NanoQuant Plate for protein measurements with regard to essential assay parameters such as linearity, uniformity, and reproducibility.

    This application note describes the detection of S. aureus growth using Tecan’s Infinite 200 PRO microplate reader to perform 600 nm absorbance measurements. The influence of assay parameter and instrument settings on data quality is discussed.

    This note describes the implementation of the Infinite 200 PRO multimode reader and the associated NanoQuant Plate for fluorescence-based DNA quantification in small volume samples using Pico Green®, an ultra-sensitive fluorescent dye for the quantitation of double-stranded DNA.

    This note describes the use of the fluorescence microplate reader Tecan Infinite F200 PRO or M200 PRO, for detection of as lias 20 pg dsDNA in a 200 μl assay volume.

    This application note describes the use of the Infinite® F200 PRO in combination with a commercially available ORAC assay kit, using different beverages as antioxidant samples.

    One of the most used methods for determining nucleic acid concentrations is based on measuring the optical density of a sample at 260 nm (OD260). According to the Lambert-Beer law, the amount of absorbed light is proportional to the concentration of the sample, and to the pathlength the light has to pass through when going through the sample.

    Infinite M Plex

    Fully loaded multimode plate reader with monochromator-based optics, offering free wavelength choice and scanning capabilities. Your ready-to-go workhorse for all standard absorbance-, fluorescence- and luminescence assays.

    Features and technologies

    Specifically optimized for wavelength accuracy and precision, the Infinite M Plex uses Quad4 Monochromators™ technology to deliver performance and versatility for absorbance-, fluorescence- and luminescence-based assays.

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    The Infinite M Plex is the adjustable workhorse for your lab, delivering the freedom to choose any assay at any time.

    The system’s monochromators give you free wavelength choice and scanning capabilities, in both absorbance and fluorescence modes. This is combined with a dedicated luminescence module – enabling glow, flash and dual-color applications – providing an outstanding combination of value and functionality in one proven instrument.

    Key applications

    • Absorbance- and fluorescence-based DNA/RNA quantification and purity checks
    • Absorbance- and fluorescence-based protein quantification
    • Absorbance-, fluorescence- and luminescence-based ELISAs
    • 600 nm growth curves (bacteria, yeast)
    • ATP quantification
    • Fluorescent and luminescent reporter assays
    • Dual-color luminescence (BRET2™, NanoBRET™, etc.)
    • Fluorescence- and luminescence-based Ca2+ release assays
    • Dual Luciferase Reporter® assays
    • Cell-based assays (viability, cytotoxicity, etc.)
    • Enzyme kinetics
    • Compound characterization (absorbance and fluorescence scans)

    Literature

    The NanoQuant Plate from Tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. Additionally, the efficiency of the nucleic acid labeling with dyes can be easily determined with this tool.

    Siloam Biosciences’ OptiMax microplate (based on the Optimiser technology platform) offers rapid, sensitive and specific chemifluorescence-based ELISA procedures using exceedingly small sample volumes. The speed, sensitivity and small sample requirements are achieved as a result of the unique microfluidic design of the Optimiser technology.

    In this note we describe the use of Tecan’s Infinite F200 PRO and Infinite M200 PRO instruments for easy and sensitive protein quantification using different absorbance-based assays.

    The isolation of adult stem cells results in very low amounts of cells due to their rare incidence in already formed tissues and organs. Here we describe the preparation and evaluation of RNA samples from a novel population of mouse keratinocyte stem cells marked by Lgr5+ expression. We used tools specifically designed for handling and measuring low RNA quantities including RNA purification and the subsequent quantification with the Infinite M200 PRO.

    Tecan´s NanoQuant detection technology has been developed to facilitate the quantification of biomolecules in small-volume samples. The present note describes the implementation of the NanoQuant Plate for protein measurements with regard to essential assay parameters such as linearity, uniformity, and reproducibility.

    This application note describes the detection of S. aureus growth using Tecan’s Infinite 200 PRO microplate reader to perform 600 nm absorbance measurements. The influence of assay parameter and instrument settings on data quality is discussed.

    This note describes the implementation of the Infinite 200 PRO multimode reader and the associated NanoQuant Plate for fluorescence-based DNA quantification in small volume samples using Pico Green®, an ultra-sensitive fluorescent dye for the quantitation of double-stranded DNA.

    This note describes the use of the fluorescence microplate reader Tecan Infinite F200 PRO or M200 PRO, for detection of as lias 20 pg dsDNA in a 200 μl assay volume.

    This Technical Note describes the relevant instrument parameters to acquire optimal fluorescence excitation and emission spectra on the Infinite M200 PRO.

    In this Application Note the three assay systems introduced above (i.e. MTT assay, CellTiter96 AQueous One Solution Cell Proliferation assay (MTS) and CellTiter-Blue Cell Viability Assay (resazurin) are compared using the human cholangiocarcinoma cell line CCLP-1. The compatibility of these assay systems with Tecan´s Infinite M200 PRO Quad4 Monochromators-based multimode reader is demonstrated.

    The BacTiter-Glo Microbial Cell Viability Assay was tested on the Infinite F200 PRO filter-based multimode reader, using the instrument’s highly sensitive luminescence module.

    This note describes the Dual Luciferase Reporter Assay performance using Tecan’s Infinite 200 PRO multimode reader equipped with a two channel injector.

    Dynamic interactions between proteins are key mediators of multiple cellular functions and are involved in almost all biological processes. Accordingly, they are valuable targets for the development of novel drug therapies. In this context, bioluminescence resonance energy transfer (BRET), developed by Promega, is a well-established detection technology for the investigation of protein-protein interactions in living cells. However, its application has so far been somewhat hampered by its limited dynamic range and sensitivity.

    One of the most used methods for determining nucleic acid concentrations is based on measuring the optical density of a sample at 260 nm (OD260). According to the Lambert-Beer law, the amount of absorbed light is proportional to the concentration of the sample, and to the pathlength the light has to pass through when going through the sample.

    Infinite F Plex

    Fully loaded multimode plate reader with filter-based optics – offering highest sensitivity within the Infinite 200 PRO family, combined with the broadest range of read modes. Your ideal system for low to medium throughput drug discovery, including HTRF® and Fluorescence Polarization.

    Features and technologies

    The Infinite F Plex delivers value without compromising on performance, combining filter-based optics for absorbance and fluorescence with a dedicated luminescence module.

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    The Infinite F Plex is the practical workhorse of the lab, delivering high sensitivity for your targeted assays.

    The filter-based optics give you increased sensitivity for both absorbance and fluorescence measurements, with a separate luminescence module to support glow, flash and dual-color applications. This proven instrument delivers more sensitivity than you might expect, while offering unmatched value.

    It is fully certified for HTRF® and helps to ensure high quality results for demanding fluorescence-based assays, such as fluorescence polarization. It also represents a reliable, cost-efficient option for exploring high throughput screening applications in your lab.

    Key applications

    • Absorbance- and fluorescence-based DNA/RNA quantification and purity checks
    • Absorbance- and fluorescence-based protein quantification
    • Absorbance-, fluorescence- and luminescence-based ELISAs
    • 600 nm growth curves (bacteria, yeast)
    • ATP quantification
    • Fluorescence- and luminescence-based reporter assays
    • Dual-color luminescence (BRET2™, NanoBRET™, etc.)
    • Dual Luciferase Reporter® assays
    • Fluorescence- and luminescence-based Ca2+ release assays
    • Enzyme kinetics
    • ORAC assays
    • Cell-based assays (viability, cytotoxicity, etc.)
    • Fluorescence polarization
    • FRET
    • Time resolved fluorescence (eg. DELFIA®)
    • TR-FRET (eg. HTRF, LanthaScreen®, Transcreener®)

    Literature

    The NanoQuant Plate from Tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. Additionally, the efficiency of the nucleic acid labeling with dyes can be easily determined with this tool.

    Siloam Biosciences’ OptiMax microplate (based on the Optimiser technology platform) offers rapid, sensitive and specific chemifluorescence-based ELISA procedures using exceedingly small sample volumes. The speed, sensitivity and small sample requirements are achieved as a result of the unique microfluidic design of the Optimiser technology.

    In this note we describe the use of Tecan’s Infinite F200 PRO and Infinite M200 PRO instruments for easy and sensitive protein quantification using different absorbance-based assays.

    The isolation of adult stem cells results in very low amounts of cells due to their rare incidence in already formed tissues and organs. Here we describe the preparation and evaluation of RNA samples from a novel population of mouse keratinocyte stem cells marked by Lgr5+ expression. We used tools specifically designed for handling and measuring low RNA quantities including RNA purification and the subsequent quantification with the Infinite M200 PRO.

    Tecan´s NanoQuant detection technology has been developed to facilitate the quantification of biomolecules in small-volume samples. The present note describes the implementation of the NanoQuant Plate for protein measurements with regard to essential assay parameters such as linearity, uniformity, and reproducibility.

    This application note describes the detection of S. aureus growth using Tecan’s Infinite 200 PRO microplate reader to perform 600 nm absorbance measurements. The influence of assay parameter and instrument settings on data quality is discussed.

    This note describes the use of the fluorescence microplate reader Tecan Infinite F200 PRO or M200 PRO, for detection of as lias 20 pg dsDNA in a 200 μl assay volume.

    This note describes the implementation of the Infinite 200 PRO multimode reader and the associated NanoQuant Plate for fluorescence-based DNA quantification in small volume samples using Pico Green®, an ultra-sensitive fluorescent dye for the quantitation of double-stranded DNA.

    This application note describes the use of the Infinite® F200 PRO in combination with a commercially available ORAC assay kit, using different beverages as antioxidant samples.

    In this Application Note the three assay systems introduced above (i.e. MTT assay, CellTiter96 AQueous One Solution Cell Proliferation assay (MTS) and CellTiter-Blue Cell Viability Assay (resazurin) are compared using the human cholangiocarcinoma cell line CCLP-1. The compatibility of these assay systems with Tecan´s Infinite M200 PRO Quad4 Monochromators-based multimode reader is demonstrated.

    The present application note summarizes the results of experiments with the HTRF (Homogeneous Time-Resolved Fluorescence, Cisbio Bioassays) technology on Infinite F200 PRO.

    The present application note summarizes the results of experiments with the HTRF (Homogeneous Time-Resolved Fluorescence, Cisbio Bioassays) technology on Infinite F200 PRO.

    The Transcreener ADP2 TR-FRET Red Assay is intended for the detection of ADP production by any kinase or ATPase, and has been developed specifically for high throughput screening applications. The assay is based on the competitive binding of ADP to a monoclonal antibody-terbium conjugate, and uses a homogeneous TR-FRET detection mechanism.

    This Technical Note describes the successful implementation of Invitrogen’s LanthaScreen TR-FRET assay system on Tecan’s Infinite F200 PRO filter-based multimodular detection system. The Infinite F200 has performed according to Invitrogen’s LanthaScreen certification program criteria and was successfully validated by Invitrogen as “LanthaScreen® Certified”.

    This Technical Note describes optimized test set-ups, instrument settings and the statistical equations that were used to determine the maximum instrument sensitivity of the Infinite F200 PRO filter-based microplate reader and the Infinite M200 PRO Quad4 Monochromators-based microplate reader for flash and glow luminescence.

    The BacTiter-Glo Microbial Cell Viability Assay was tested on the Infinite F200 PRO filter-based multimode reader, using the instrument’s highly sensitive luminescence module.

    This note describes the Dual Luciferase Reporter Assay performance using Tecan’s Infinite 200 PRO multimode reader equipped with a two channel injector.

    Dynamic interactions between proteins are key mediators of multiple cellular functions and are involved in almost all biological processes. Accordingly, they are valuable targets for the development of novel drug therapies. In this context, bioluminescence resonance energy transfer (BRET), developed by Promega, is a well-established detection technology for the investigation of protein-protein interactions in living cells. However, its application has so far been somewhat hampered by its limited dynamic range and sensitivity.

    One of the most used methods for determining nucleic acid concentrations is based on measuring the optical density of a sample at 260 nm (OD260). According to the Lambert-Beer law, the amount of absorbed light is proportional to the concentration of the sample, and to the pathlength the light has to pass through when going through the sample.

     

     

    Upgrade paths for the Infinite 200 PRO plate reader family.

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    Brochure

    The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications.

    Read the infinite brochure
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    Typical performance values

    Infinite 200 PRO instrument specifications and typical performance values...

    See the typical performance values
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    Trustmarks

     

    DLReady LanthaScreen Transcreener Transcreener HTRF