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Comparison of Absorbance- and Multimode readers

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  Sunrise™ Infinite® F50 / Robotic Infinite M Nano Infinite  Lumi
  Sunrise™ Infinite® F50 / Robotic Infinite® M Nano Infinite® Lumi
Wavelength selection
Filter-based instrument        
Tunable wavelength selection  
Tunable gradient filter
   
Quad4 monochromator™
 
 
Detection modes
Absorbance endpoint & kinetics
spectral range
340-750 nm 400-750 nm 230-1000 nm  
Absorbance scans
spectral range
400-700nm   230-1000nm  
Absorbance multichannel detection 12
channels
8
channels
1
channel
 
Luminescence
spectal range
      380-600 nm
Glow luminescence        
Flash luminescence        
Dual Color luminescence        
 
Plate formats
Number of wells 96 96 6-384 6-384
NanoQuant Plate™        
Cuvettes        
 
Further options
Temperature control up 42°C        
Barcode scanner        
Upgradeable        
QC tools        
 
User interfaces
Workflow oriented i-control™ software        
Magellan™ data analysis software        
 
Exemplified Applications
ELISA        
Agglutination reading        
Nucleic acid quantification*        
Protein quantification        
Labelling efficency        
ATP detection        
Reporter assays        
Ca2+ release        
 
Ready for automation
Connect™ plate stacker        
Freedom EVO®        
Fluent®        
 
Designed to meet regulatory needs
98/79/EC IVD-D        
21 CFR Part 11 support**        
2011/65/EU RoHS        
* via 260nm absorbance
** Using with Magellan Tracker
  Spark Cyto Spark Infinite M Nano Plus Infinite F Nano Plus Infinite M Plex Infinite F Plex
  Spark® Cyto*
 
Spark
 
Infinite®
M Nano Plus
Infinite®
F Nano Plus
Infinite®
M Plex
Infinite®
F Plex
Wavelength selection
Filter-based wavelength
selection
 
incl. 3+1 dichroics
 
incl. 3+1 dichroics
       
Quad4 monochromators™
(tunable wavelength)
           
QuadX monochromators™
(tunable wavelength and bandwidth)
           
Fusion™ optics
(filter and Quad4/Quad X monochromators)
           
 
Labware formats
Microplates 1-1536 1-1536 6-384 6-384 6-384 6-384
Cuvette adapter plate            
Cuvette port            
NanoQuant Plate™ compatible
(2µl micro volume plate)
           
Cell Chips™ cell counting slides            
 
Detection modes
Absorbance endpoint +
Kinetic spectral range
200–1,000 nm 200–1,000 nm 230–1,000 nm 230–1,000 nm 230–1,000 nm 230–1,000 nm
Absorbance spectral scan            
Fluorescence top and bottom
reading spectral range
Ex 230-900 nm
Em 280-900 nm
Ex 230-900 nm
Em 280-900 nm
Ex 230–850 nm
Em 280-850 nm
Ex 230–850 nm
Em 280-850 nm
Ex 230-850 nm
Em 280-850 nm
Ex 230-850 nm
Em 280-850 nm
Fluorescence spectral scan            
Adjustable bandwidth in fluorescence            
Automated z-focusing top            
Automated z-focusing bottom            
Fluorescence Polarization (FP)            
Time-resolved fluorescence (TRF)            
Fluorescence resonance
energy transfer (FRET)
           
Time-resolved fluorescence
resonance energy transfer TR-FRET
           
Multiple reads per well            
Optimal read in fluorescence bottom            
Well area scan
(100x100 spots/well)
           
Flash and glow luminescence incl. BRET            
Luminescence spectral scan
(tunable wavelength)
           
AlphaScreen®/AlphaLISA®/AlphaPlex®            
Automated cell counting in Cell chips™            
Brightfield            
Digital phase contrast imaging            
Fluorescence imaging            
 
Incubation
Temperature control up to 42°C            
Active cooling below ambient
temperature (Te-Cool™)
           
CO2 and O2 incubation option
(GCM™ gas control module)
           
Humidity control
(evaporation protection)
           
 
Further Options
Two reagent injectors            
Injector with heater and stirrer            
Automated Lidlifter™            
Barcode scanner            
Upgradeable            
IQ/OQ and QC tools            
21 CFR Part 11 support**            
 
User interfaces
i-control™ / SparkControl™
Reader confirmed software
           
Magellan™ data analysis software            
Image Analyzer™ software            
 
Ready for automation
Spark Stack            
Freedom EVO®            
Fluent®            
 
Applications
Nucleic acid quantification            
Protein quantification            
ATP assays            
Genereporter assays            
BRET applications            
HTRF®            
DLReady™            
Transcreener® FI            
Transcreener® FP            
Transcreener® TR-FRET            
Confluence assessment            
Nuclei counting via Hoechst            
GFP transfection efficiency            
Cell viability via Calcein/PI            
Cell death via Annexin V            
 
* Capabilities depend on the Spark Cyto configurations
** Using with Magellan Tracker
The Infinite and Spark multimode microplate readers are modular and upgradeable. Accordingly there are different configurations possible. Certain functionalities in the table above requires specific configurations.

Please talk to your your local Tecan representative for the right configuration which suits your application demands.