NGS DreamPrep™

Walkaway automation solution for NGS library prep

NGS DreamPrep is a fully optimized solution for walkaway NGS library prep and QC. It minimizes manual intervention, saving time and delivering consistent, sequencing-ready libraries for a wide range of research applications.

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Tab 01 / Complete solution
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NGS DreamPrep combines the Fluent® Automation Workstation, Infinite® F Nano+ plate reader and Tecan Genomics’ library preparation kits to provide sequencing-ready libraries with unprecedented speed and accuracy. This groundbreaking approach offers preparation, quantification, normalization and pooling of NGS libraries in one automated workflow.

Tecan’s innovative NuQuant® library quantification method uses the integrated Infinite reader to enable fully automated QC without sample loss in under six minutes, eliminating the need for slow and costly qPCR- or capillary electrophoresis-based QC.

Each NGS DreamPrep workstation is supplied with example methods* for Tecan Genomics reagent kits, including AnyDeplete™ mediated transcript depletion of unwanted reads from sequencing data.

NGS DreamPrep

System highlights

  • DNA-Seq libraries, normalized and ready to sequence, in less than 4 hours
  • mRNA-Seq libraries, quantified and ready to sequence in 9 instead of 14 hours
  • Integrated, full library QC in less than 6 minutes without sample loss
  • Addition only workflows for DNA-Seq, plus minimal intervention for RNA-Seq
  • Single point of contact for automation, reagents, consumables, application support, etc.
  • Intuitive, touchscreen-based user interface that can be customized to your needs

* Example methods have not been validated. Please make a copy of this method in order to customize it to accommodate and validate your workflow according to your desired intended use and laboratory protocols. Tecan makes no claims regarding the performance of the example methods.




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Tab 02 / Automation
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Rapid technology advances have made NGS a reliable and simple option for genomic assays, but library preparation remains a challenge for high throughput applications. The speed, accuracy and precision of the Fluent platform make it ideally suited to preparing NGS libraries, reducing process variability and ensuring greater reproducibility between operators and over time.

The system’s three independent, task-specific arms operate in parallel to provide fast, efficient processing. The built-in touchscreen interface guides users through daily tasks, and can be easily customized to suit your laboratory and workflow needs for straightforward operation. Together with integrated user management and Method Approval functions, this ensures secure protocols and optimal performance.

On-deck thermocycling for longer walkaway runs

Integrated plate reader for QC without sample loss

Magnetic separation for bead clean-ups

Method Approval function ensures compliance with SOPs and validated protocols

Intuitive touchscreen interface

Parallel processing with three task-specific arms maximizes throughput




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Tab 03 / Detection
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Sensitive and fast quantification

The Infinite F Nano+ multimode reader offers absorbance and high sensitivity fluorescence reading modes in one compact instrument.

It combines the high sensitivity of fluorescence assays and the speed provided by reading plate formats of up to 384-wells.

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NGS library quantification is a crucial step for a successful sequencing run. Accurately determining the number of sequenceable molecules becomes highly significant for new high capacity sequencers such as the NovaSeq.

NuQuant is a novel library quantification method, which accurately measures molar library concentration without the need for separate fragment size analysis. This proprietary method uses a fluorescent label which is incorporated into the library molecules during library preparation. NuQuant is independent of library size, allowing library molarity to be directly measured using fluorometers such as the Infinite F Nano+.

Integrated library QC in less than 6 minutes - Quantification of the libraries using NuQuant on the plate reader eliminates the need of additional manual qPCR or capillary electrophoresis steps to quantify libraries, saving significant time, resources and valuable library material.

NGS Dreamprep - Fluent

After final purification of DNA libraries in a 96-well plate with the Fluent, the fluorescence of the libraries and standards is measured on the Infinite plate reader. The molarity determined for each library can then be used for balanced pooling and subsequent sequencing on Illumina instruments.




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Tab 04 / Reagents
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The NGS DreamPrep is a complete solution designed and optimized for completely automated DNA library preparation and quantification. It combines Celero DNA-Seq and Universal Plus mRNA-Seq library preparation kits with a Fluent workstation and integrated Infinite® plate reader, allowing users to transform up to 96 DNA samples into readily normalized libraries.

Celero DNA-Seq

Celero PCR Workflow with Enzymatic Fragmentation DNA-Seq library preparation kit is a simple single tube, addition only workflow with just one post-amplification bead purification step. Celero DNA libraries can be prepared, quantified, normalized and pooled in less than four hours on the Fluent Automation Workstation, compared to more than seven hours for library preparation and qPCR quantification with other kits. Key highlights of this DNA-Seq library preparation kit includes:

  • Broad dynamic input range 10 ng to 500 ng
  • Addition only, three step workflow
  • Robust enzymatic fragmentation
  • DimerFree technology eliminates adaptor dimers
  • NuQuant library quantification method integrated at no additional cost to accurately quantify library concentrations (in nM)

NGS Dreamprep - Celero Workflow

Celero library preparation kit offers a simple, addition-only workflow for generating quantified libraries ready for sequencing.

Universal Plus mRNA-Seq

Universal Plus mRNA-Seq library preparation kit features a robust end-to-end workflow with a wide input range for analysis of coding transcripts. Universal Plus mRNA libraries can be prepared, quantified, normalized and pooled in less than nine hours on the Fluent Automation Workstation, compared to more than fourteen hours for manual or automated library preparation and qPCR quantification with other kits. Key features of this mRNA-Seq library preparation kit include:

  • Broad dynamic input rage from 10 ng to 1 µg
  • DimerFree technology eliminates adaptor dimers
  • NuQuant library quantification method integrated at no additional cost to accurately quantify library concentrations (in nM)
  • Integrated AnyDeplete technology aides in removal of unwanted transcripts post library preparation to maximize informative sequencing reads

NGS Dreamprep - Steps Workflow

Universal Plus mRNA-Seq kit offers a streamlined workflow for the generation of sequencing ready quantified libraries. The workflow can include a customizable transcript depletion step.




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Tab 05 / Literature
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Brochures

NGS DreamPrep is a complete, walkaway solution that combines the Fluent® Automation Workstation, the Infinite® F Nano+ plate reader and Tecan Genomics’ innovative library preparation kits to create sequencing-ready libraries with minimal manual intervention.

Application notes

The Celero PCR Workflow with Enzymatic Fragmentation DNA-Seq Kit offers a simple, addition-only workflow for generating quantified libraries ready for sequencing.

The Universal Plus mRNA-Seq library preparation kit with NuQuant offers a streamlined workflow for the generation of sequencingready quantified libraries

NuQuant® is a novel library quantification method, which accurately measures molar library concentration without the need for separate fragment size analysis.

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