NGS DreamPrep™

Rapid technology advances have made NGS a reliable and simple option for genomic assays, but library preparation remains a challenge for high throughput applications. The speed, accuracy and precision of the Fluent platform make it ideally suited to preparing NGS libraries, reducing process variability and ensuring greater reproducibility between operators and over time.

The system’s three independent, task-specific arms operate in parallel to provide fast, efficient processing. The built-in touchscreen interface guides users through daily tasks, and can be easily customized to suit your laboratory and workflow needs for straightforward operation. Together with integrated user management and Method Approval functions, this ensures secure protocols and optimal performance.

NGS library quantification is a crucial step for a successful sequencing run. Accurately determining the number of sequenceable molecules becomes highly significant for new high capacity sequencers such as the NovaSeq.

NuQuant is a novel library quantification method, which accurately measures molar library concentration without the need for separate fragment size analysis. This proprietary method uses a fluorescent label which is incorporated into the library molecules during library preparation. NuQuant is independent of library size, allowing library molarity to be directly measured using fluorometers such as the Infinite F Nano+.

Integrated library QC in less than 6 minutes - Quantification of the libraries using NuQuant on the plate reader eliminates the need of additional manual qPCR or capillary electrophoresis steps to quantify libraries, saving significant time, resources and valuable library material.

After final purification of DNA libraries in a 96-well plate with the Fluent, the fluorescence of the libraries and standards is measured on the Infinite plate reader. The molarity determined for each library can then be used for balanced pooling and subsequent sequencing on Illumina instruments.

The NGS DreamPrep is a complete solution designed and optimized for completely automated DNA library preparation and quantification. It combines Celero DNA-Seq and Universal Plus mRNA-Seq library preparation kits with a Fluent workstation and integrated Infinite® plate reader, allowing users to transform up to 96 DNA samples into readily normalized libraries.

Celero DNA-Seq

Celero PCR Workflow with Enzymatic Fragmentation DNA-Seq library preparation kit is a simple single tube, addition only workflow with just one post-amplification bead purification step. Celero DNA libraries can be prepared, quantified, normalized and pooled in less than four hours on the Fluent Automation Workstation, compared to more than seven hours for library preparation and qPCR quantification with other kits. Key highlights of this DNA-Seq library preparation kit includes:

• Broad dynamic input range 10 ng to 500 ng
• Addition only, three step workflow
• Robust enzymatic fragmentation
• DimerFree technology eliminates adaptor dimers
• NuQuant library quantification method integrated at no additional cost to accurately quantify library concentrations (in nM)

Celero library preparation kit offers a simple, addition-only workflow for generating quantified libraries ready for sequencing.

Universal Plus mRNA-Seq

Universal Plus mRNA-Seq library preparation kit features a robust end-to-end workflow with a wide input range for analysis of coding transcripts. Universal Plus mRNA libraries can be prepared, quantified, normalized and pooled in less than nine hours on the Fluent Automation Workstation, compared to more than fourteen hours for manual or automated library preparation and qPCR quantification with other kits. Key features of this mRNA-Seq library preparation kit include:

• Broad dynamic input rage from 10 ng to 1 µg
• DimerFree technology eliminates adaptor dimers
• NuQuant library quantification method integrated at no additional cost to accurately quantify library concentrations (in nM)
• Integrated AnyDeplete technology aides in removal of unwanted transcripts post library preparation to maximize informative sequencing reads

Universal Plus mRNA-Seq kit offers a streamlined workflow for the generation of sequencing ready quantified libraries. The workflow can include a customizable transcript depletion step.