Scientific Citations

More than ### citations published since the beginning of 2017.

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1255

Total number of publications since 2017

159

Average number of new publications per year

Average number of new publications per month

Vitamin B12 modulates Parkinson's disease LRRK2 kinase activity through allosteric regulation and confers neuroprotection

Fully Printed Infrared Photodetectors from PbS Nanocrystals with Perovskite Ligands

Multiplexed precision genome editing with trackable genomic barcodes in yeast

Transcriptional addiction in cancer cells is mediated by YAP/TAZ through BRD4

A molecular roadmap for the emergence of early-embryonic-like cells in culture

Reticular adhesions are a distinct class of cell-matrix adhesions that mediate attachment during mitosis

Automated Buildup of Biomimetic Films in Cell Culture Microplates for High-Throughput Screening of Cellular Behaviors

Polarity Acquisition in Cortical Neurons Is Driven by Synergistic Action of Sox9-Regulated Wwp1 and Wwp2 E3 Ubiquitin Ligases and Intronic miR-140

Convergent genomic signatures of domestication in sheep and goats

A printable hydrogel microarray for drug screening avoids false positives associated with promiscuous aggregating inhibitors

Vitamin B12 modulates Parkinson's disease LRRK2 kinase activity through allosteric regulation and confers neuroprotection

This TR-FRET-based assay was used in a high-throughput screen (HTS) of small molecule chemical compounds for LRRK2 using the TECAN (Mannedorf, Switzerland) Freedom EVO 200 liquid handling system, and relative fluorescence was measured using the TECAN Safire 2 fluorescence spectrometer.

Fully Printed Infrared Photodetectors from PbS Nanocrystals with Perovskite Ligands

For the deposition of the PbS nanocrystal ink within ABS frames a Freedom Evo 100 (Tecan Group AG, Switzerland), a pipetting robot was used, whereby the system was handled by the software EVOware Standard (version 2.7).

Multiplexed precision genome editing with trackable genomic barcodes in yeast

... (Torrey Pines) for further processing. The sub-passage and culture sampling steps were triggered by a pre-defined OD (0.6), not by time elapsed. Liquid transfers were performed automatically using a Freedom EVO liquid handling system (Tecan), which was controlled by custom Pegasus software (Tecan). For the colony count analysis for survival on 5-fluoro-cytosine (5-FC) versus YPD, a strain harbori ... rol of Cas9 expression. Analysis of editing, barcoding, and plasmid removal kinetics For experiments described in Figure 2a and 2b, cells were cultured in 48-well plates in an Infinite plate reader (Tecan) at 30Â°C with orbital shaking. OD600 was followed by taking measurements every ~15 minutes. Cultures were maintained in log phase growth by passaging cultures every 2 doublings, when an aliquo

Transcriptional addiction in cancer cells is mediated by YAP/TAZ through BRD4

... tected using the Fast Red detection reagent. RT-qPCR Total RNA extraction from cells and tissue was performed with NucleoSpin 8 RNA Core Kit (Macherey-Nagel, REF.740465.4) using an automated system (Freedom EVO, Tecan). Contaminant DNA was removed by rDNase Set (Macherey-Nagel, REF.740963). Pancreatic acini were harvested in TriPure (Roche) for total RNA extraction.cDNA synthesis was carried out ... e treated with different doses of JQ1 (1nM, 10nM, 0.1ÂľM and 1ÂľM). Cells were harvested after 24h of treatment (day2). Firefly luciferase activity was measured with an Infinite F200PRO plate reader (TECAN), using Luciferin (Sigma) as substrate. Data are presented as firefly/Î˛-gal activity. Each sample was transfected in duplicate and each experiment was repeated at least three times independentl

A molecular roadmap for the emergence of early-embryonic-like cells in culture

... and OCT4 antibodies followed by Alexa Fluor 647, 555 and 488 conjugated second antibodies (Invitrogen). Cell nuclei were counterstained with 1 Îźg/mL DAPI. The screening was achieved owing to a Tecan Freedom EVO 150 (for cell transfection, staining, and immunocytochemistry) and to an Orbitorâ˘ RS Microplate Mover robotic arm coupling microplate stacks to a Cellomics CellInsightâ˘ NXT High-Conten ... SCAN4 and OCT4 antibodies followed by Alexa Fluor 647, 555 and 488 conjugated second antibodies (Invitrogen). Cell nuclei were counterstained with 1 Îźg/mL DAPI. The screening was achieved owing to a Tecan Freedom EVO 150 (for cell transfection, staining, and immunocytochemistry) and to an Orbitorâ˘ RS Microplate Mover robotic arm coupling microplate stacks to a Cellomics CellInsightâ˘ NXT High-

Reticular adhesions are a distinct class of cell-matrix adhesions that mediate attachment during mitosis

Immunofluorescence labelling was performed either manually or using liquid-handling robotics (Freedom EVO, Tecan) to minimize experimental variability, as described previously59.

Automated Buildup of Biomimetic Films in Cell Culture Microplates for High-Throughput Screening of Cellular Behaviors

LbL films were directly deposited in 96âwell cell culture microplates (Greiner BioâOne, Germany) using an automated liquid handling machine (TECAN Freedom EVO 100, Tecan France, Lyon).

Polarity Acquisition in Cortical Neurons Is Driven by Synergistic Action of Sox9-Regulated Wwp1 and Wwp2 E3 Ubiquitin Ligases and Intronic miR-140

Synaptosomal protein fractions (P2C) corresponding to 20 mg protein were dissolved in lysis buffer and processed according to a filter-aided sample preparation (FASP) protocol modified essentially as described by Distler et al. Unless stated otherwise, all steps were automated on a liquid-handling workstation equipped with a vacuum manifold (Freedom EVO 150, Tecan) by using an adaptor device constructed in-house

Convergent genomic signatures of domestication in sheep and goats

... protocol. Tissue sampling was performed in MN square-well blocks to obtain 25 mg fragments per sample. Three and a half MN square-96 blocks were prepared, and extraction was performed using a Tecan Freedom Evo Liquid handler following manufacturer's protocol. A pre-lysis step was carried out to homogenize samples with 180 Âľl of T1 Buffer and 25 Âľl of proteinase K overnight at 56 Â°C. To ... rer's protocol. Tissue sampling was performed in MN square-well blocks to obtain 25 mg fragments per sample. Three and a half MN square-96 blocks were prepared, and extraction was performed using a Tecan Freedom Evo Liquid handler following manufacturer's protocol. A pre-lysis step was carried out to homogenize samples with 180 Âľl of T1 Buffer and 25 Âľl of proteinase K overnight at 56 Â°

A printable hydrogel microarray for drug screening avoids false positives associated with promiscuous aggregating inhibitors

... ozone plate as described previously. An aliquot of 5 Îźl of tazobactam, sulbactam, and clavulanic acid solutions (at starting concentrations of 100 ÎźM) were added to each microzone using a Tecan Freedom Evo 200 liquid-handling robot (Tecan, Switzerland). The inhibitor was incubated with the printed Î˛-lac for 20 min, after which the assay was initiated with the addition of 5 Îźl of nitroc ... of 200 ÎźM. Î˛-lac activity was then assessed via UV-vis spectrophotometry by tracking the hydrolysis of nitrocefin by monitoring solution absorbance at 492 nm (Infinite M1000 spectrophotometer, Tecan). Printed hydrogel-based Î˛-lac assay POH ink solution was prepared with a final concentration of 50 nM Î˛-lac and used to print hydrogel spots on a 96-well paper microzone plate as described

Selective Synthesis of the Human Drug Metabolite 5â˛-Hydroxypropranolol by an Evolved Self-Sufficient Peroxygenase

Aliquots of the supernatants (20 ÎźL) were transferred from the master plates to two replica plates with the aid of a liquid handler robotic station Freedom EVO (Tecan, Switzerland).

High-throughput microsphiltration to assess red blood cell deformability and screen for malaria transmission-blocking drugs

The Biomek dispenser series has been selected to prepare microsphiltration plates in different international laboratories28, but other dispensers of equivalent hydraulic power and functionalities can be used, as for instance, those of the Freedom EVO series (Tecan)15.

Common genetic variation drives molecular heterogeneity in human iPSCs

In total, 4 Îźl (200 ng) of DNA is required for the pre-amplification reaction using a Tecan Freedom Evo.

The Centrosome Is a Selective Condensate that Nucleates Microtubules by Concentrating Tubulin

Two separate solutions containing 50-200 nM SPD-5 in Condensate buffer and 6%â24% PEG-3350 in Condensate buffer were dispensed into two rows of a 96 well plate. A pipetting robot (Tecan, Model Freedom EVO 200) equipped with a TeMo 96 pipetting head with disposable tips (50 ml) was used to mix the SPD-5 and PEG solutions 1:1 and then transfer each condition in quadruplicate to a 384 well plastic bottom imaging plate (15 mL per well) to synchronize condensate formation

Coordinated Splicing of Regulatory Detained Introns within Oncogenic Transcripts Creates an Exploitable Vulnerability in Malignant Glioma

... rug DMSO control point) was plated in a 96-well plate 2-fold dilution steps, starting at 500 mM. 100 nl of the compound solutions were pin-transferred (V&P Scientific, CA, pin tool mounted onto Tecan Freedom Evo 150 MCA96 head, Tecan, CA) into 96-well plates and incubated for 120h. Cells were seeded in 96-plates on the day prior to drug treatment. Seeding cell numbers were adjusted for different c ... e no-drug DMSO control point) was plated in a 96-well plate 2-fold dilution steps, starting at 500 mM. 100 nl of the compound solutions were pin-transferred (V&P Scientific, CA, pin tool mounted onto Tecan Freedom Evo 150 MCA96 head, Tecan, CA) into 96-well plates and incubated for 120h. Cells were seeded in 96-plates on the day prior to drug treatment. Seeding cell numbers were adjusted for diffe

Small-molecule inhibition of STOML3 oligomerization reverses pathological mechanical hypersensitivity

Overnight monitoring of signal development was performed with a Freedom Evo workstation, a SafireII plate reader for fluorescence measurement (Tecan Group Ltd, MĂ¤nnedorf, Switzerland) and an integrated STX44-ICSA automated plate incubator (Liconic AG, Mauren, Liechtenstein).

Systematic Investigation of Transcription Factor Activity in the Context of Chromatin Using Massively Parallel Binding and Expression Assays

All steps from this point were carried on out using a programmable robotic system (Tecan Freedom Evo, Tecan Inc.)

Chromosomal contacts connect loci associated with autism, BMI and head circumference phenotypes

... ontamination. qPCR experiments were performed in triplicate using SYBR-Green (Roche, Basel, Switzerland) as reporter. The reaction mixtures were prepared in 384-well plates using a Freedom Evo robot (Tecan, MĂ¤nnedorf, Switzerland) and run in an ABI 7900HT sequence detection system (Applied Biosystems) using the following conditions: 50Â°C for 2 min, 95Â°C for 10 min, followed by 45 cycles of 95

Small molecule screen in embryonic zebrafish using modular variations to target segmentation

... tribute volumes of small molecules into master plates. All working concentrations of 2, 10, and 50 ÂľM, were further diluted from one master plate per experiment for higher accuracy using the Tecan Freedom Evo (Tecan) with a 96-head and directly applied to embryos at 10 hpf at a final volume of 100 Âľl per well to start the treatment. Propylthiouracil was added at a final concentration of 0. ... to distribute volumes of small molecules into master plates. All working concentrations of 2, 10, and 50 ÂľM, were further diluted from one master plate per experiment for higher accuracy using the Tecan Freedom Evo (Tecan) with a 96-head and directly applied to embryos at 10 hpf at a final volume of 100 Âľl per well to start the treatment. Propylthiouracil was added at a final concentration

Survival of pancreatic cancer cells lacking KRAS function

50 nL of compounds were pin-transferred (V&P Scientific pin tool mounted onto a Tecan Freedom Evo 150 MCA96 head) into duplicate assay plates and incubated for 72 h. The DMSO content was 0.1% within each well.

Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles

Cell sorting, lysis, and gDNA amplification setup were performed in a HEPAfiltered environment conforming to Class 1000 cleanroom specifications. Prior to cell sorting, the instrument, the reagents, and the workspace were decontaminated for DNA using UV irradiation and sodium hypochlorite solution, as previously described61. To further reduce the risk of DNA contamination, and to improve accuracy and throughput, Bravo (Agilent Technologies) and Freedom Evo (Tecan) robotic liquid handlers were used for all liquid handling in 384-well plates.

Cytosolic delivery of siRNA by ultra-high affinity dsRNA binding proteins

... washed with PBS, trypsinized, neutralized with cold PBSA containing 2% FBS and analyzed on an iQue Screener (IntelliCyt). All liquid handling was performed using an EL406 plate washer (BioTek) and a Freedom EVO 150 liquid handling system (Tecan) to minimize variability. Background from untreated cells were subtracted from all measurements, which were then converted to number of fluorophores using ... ed with cold PBSA containing 2% FBS and analyzed on an iQue Screener (IntelliCyt). All liquid handling was performed using an EL406 plate washer (BioTek) and a Freedom EVO 150 liquid handling system (Tecan) to minimize variability. Background from untreated cells were subtracted from all measurements, which were then converted to number of fluorophores using Quantum Alexa Fluor 647 MESF beads foll

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Scientific Citations

More than ### citations published since the beginning of 2017.

Do you want to know more?

Vitamin B12 modulates Parkinson's disease LRRK2 kinase activity through allosteric regulation and confers neuroprotection

Fully Printed Infrared Photodetectors from PbS Nanocrystals with Perovskite Ligands

Multiplexed precision genome editing with trackable genomic barcodes in yeast

Transcriptional addiction in cancer cells is mediated by YAP/TAZ through BRD4

A molecular roadmap for the emergence of early-embryonic-like cells in culture

Reticular adhesions are a distinct class of cell-matrix adhesions that mediate attachment during mitosis

Automated Buildup of Biomimetic Films in Cell Culture Microplates for High-Throughput Screening of Cellular Behaviors

Polarity Acquisition in Cortical Neurons Is Driven by Synergistic Action of Sox9-Regulated Wwp1 and Wwp2 E3 Ubiquitin Ligases and Intronic miR-140

Convergent genomic signatures of domestication in sheep and goats

A printable hydrogel microarray for drug screening avoids false positives associated with promiscuous aggregating inhibitors