NGS library quantification is a crucial step for a successful sequencing run. Accurately determining the number of sequenceable molecules becomes highly significant for new high capacity sequencers such as the NovaSeq.
NuQuant is a novel library quantification method, which accurately measures molar library concentration without the need for separate fragment size analysis. This proprietary method uses a fluorescent label which is incorporated into the library molecules during library preparation. NuQuant is independent of library size, allowing library molarity to be directly measured using fluorometers such as the Infinite F Nano+.
Integrated library QC in less than 6 minutes - Quantification of the libraries using NuQuant on the plate reader eliminates the need of additional manual qPCR or capillary electrophoresis steps to quantify libraries, saving significant time, resources and valuable library material.
After final purification of DNA libraries in a 96-well plate with the Fluent, the fluorescence of the libraries and standards is measured on the Infinite plate reader. The molarity determined for each library can then be used for balanced pooling and subsequent sequencing on Illumina instruments.