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    Automated NGS library preparation

    NGS library preparation, quality control and pooling

    From just a few samples a week to hundreds a day, automation of NGS sample preparation allows you to reproducibly create high quality libraries for reliable next-generation sequencing (NGS).

    Free Download: ONT's Ligation Sequencing Kit XL V14 automated on the DreamPrep NGS workstation

    Free Download:

    ONT's Ligation Sequencing Kit XL V14 automated on the DreamPrep® NGS workstation
    Overview
    Get a quote Talk to an expert
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    Tab 01 / Overview
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    Our NGS library prep solutions are designed to enable your needs and accelerate your science.

    Leave library prep to us, save time for what matters more.

    Validation and verification-ready Tecan’s reagents and 3rd party’s kits to develop automated NGS library prep protocols to ensure excellent performance in any lab.

    Reliable and reproducible NGS library prep

    From plug-and-play to high to highly flexible automated solutions. Now you can walk-away with confidence.

    All in one solution with proven technology and expertise.

    From reagents, automation, and consumables to application expertise – we support you every step of the way.

    Product Features

    From low to high throughput, turnkey solutions to OEM development, our comprehensive NGS library prep solutions allow scientists to save time on library prep and focus on what matters most.

    Tecan’s Reagents​
      

    • • Applicable for a broad range of sample types
    • • Inputs as low as 10 pg for DNA-Seq and RNA-Seq

    Discover more:

    Solution Brochure

    MagicPrep® NGS​
      

    • • 8 samples per run
    • • Plug & Play
    • • Used with Tecan’s NGS reagents (DNA and mRNA-seq)

    Discover more:

    Solution Brochure

    DreamPrep® NGS Compact​

    • • Up to 48 samples per run
    • • Benchtop solution
    • • Open platform compatible with several NGS protocols

    Discover more:

    Solution Brochure

    DreamPrep® NGS​
      

    • • Up to 96 samples per run
    • • High capacity
    • • Open platform compatible with several NGS protocols

    Discover more:

    Solution Brochure

    Custom Systems for OEM Partners​

    • • Designed to your requirements and your regulatory needs
    • • Optimized for reagent kits of OEM Partner

    Discover more:

    Here

    Collaborating to Advance Genomics

    We continually strive to offer innovative, accessible and always reliable solutions.

    NGS_Collaborations_v01_1140x316

     

    DreamPrep® NGS Compact

    • Up to 48 samples per run
    • Benchtop solution
    • Open platform compatible with several NGS protocols

    Discover more:

    Solution Brochure

    DreamPrep® NGS​

    • Up to 96 samples per run
    • High capacity
    • Open platform compatible with several NGS protocols

    Discover more:

    Solution Brochure

    Custom Systems for OEM Partners​

    • Designed to your requirements and your regulatory needs
    • Optimized for reagent kits of OEM Partner

    Discover more:

    Here




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    Tab 02 / DreamPrep® NGS Solutions
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    DreamPrep NGS and DreamPrep NGS Compact

    Walkaway automation solutions for NGS library prep

    Flexible configurations to deliver consistent, sequencing-ready libraries for a wide range of NGS protocols with minimal manual intervention that match your different throughput requirements.

    Both solutions capitalize on the state-of-the-art Fluent® automation workstation, delivering extraordinary precision, unmatched efficiency, and seamless integration across diverse throughputs.

    DreamPrep NGS

    DreamPrep™ NGS

    • High throughputs (up to 384 samples/day1)
    • 52 deck positions
    • Plate reader and ODTC are included for long walk-away times and full NGS workflows

    Output

    • 96 DNA libraries per run in less than 4h with Tecan NGS kit Celero™
    • 96 RNA libraries per run in 9h with Tecan NGS kit Universal Plus™ mRNA
    • Targeted DNA genotyping
      • 384 samples per day with Tecan NGS kit Allegro®
      • 384 samples per day with Tecan NGS Protocol combining Tecan NGS kit Celero and QIAseq SARS-CoV-2 Primer Panel

    Software packages included

    DreamPrep NGS Compact

    DreamPrep™ NGS Compact

    • Low-medium throughputs (8-48 samples/day1)
    • Smaller footprint, 30 deck positions
    • Three flexible configurations to cover distinct customer requirements

    Three configurations are available and upgradable at any time

    Basic: Increase your productivity as all liquid transfers and incubations are covered by the platform

    Mid: Extend your walk-away time and perform amplification steps by simply adding the ODTC

    Full: Integrate Quantification and Normalizations steps in your NGS workflows by including the Infinite® 200 Pro Reader F Nano+

    Software packages included

    Revolutionize your workflows effortlessly and embrace a new era of liquid handling excellence with our NGS solutions:

    Open and flexible platform

    All components2 provide high performance across different library preparation kits

     

    Fast QC and normalization

    Enabled by the Tecan integrated plate reader.

    Fast processing speed

    Parallel movement of robotic arms increases productivity

     

     

     

    Intuitive interaction with a touchscreen

    User-friendly interface empowers your daily operations

     

    Walk-away time solutions

    On-deck thermal cycler enables longer walk-away times

     

     

    Scale up your NGS workflows with DreamPrep NGS solutions

    These platforms combine the advanced technology of the Fluent® automation workstation, Infinite® 200 Pro Reader F Nano+ and 3rd party NGS library preparation kits to generate sequencing-ready NGS libraries in one simple automated workflow.

    DreamPrep NGS is simply fast and precise

    Save reagents and samples

    One shaker and three thermal devices for optimal library preparation

    Oxford Nanopore's Ligation Sequencing Kit XL V14 automated on the DreamPrep NGS workstation

    Enhanced reliability with Tecan consumables

    Prevent unauthorized changes to validated protocols with the Method Approval function

    1) Number of samples processed in walk-away mode is determined by the NGS protocol

    2) Ring magnet plates and POGO® adapter with Spring Cushion Technology manufactured by ALPAQUA® Engineering, LLC. Beverly, USA.
    2) Thermal devices (Cold Plate Liquid-Cooled, CPLC) and ODTC® manufactured by Inheco© Industrial Heating and Cooling GmbH. Martinsried, Germany.
    2) BioShake®3000-T elm manufactured by QInstruments® GmbH. Jena, Germany.
    2) Infinite® 200 PRO F Nano+ Reader is a product of Tecan Trading AG, Switzerland.
    3) NuQuant® is a registered trademark of Tecan Group Ltd., Männedorf, Switzerland or of Tecan Genomics, Inc., Redwood City, USA.

    Example methods have not been validated. Please make a copy of these methods to customize them to accommodate and validate your workflow according to your desired intended use and laboratory protocols. Tecan makes no claims regarding the performance of the example methods.




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    Tab 03 / Qualified NGS protocols
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    Check our list of available protocols for NGS library prep.


    Please read the method definitions in the bottom section.

    		 Veya NGS DreamPrep NGS DreamPrep NGS Compact
    Supplier Method Kit Veya®
    NGS    		 DreamPrep® NGS DreamPrep® NGS Compact
    10x Genomics | NGS | Chromium Next GEM Single Cell 5' v2 Kit | mike | DreamPrep NGS | blue | 10x Genomics
    Single cell RNA-seq    		 NGS Chromium Next GEM Single Cell 5' v2 Kit      
    Agilent | NGS | SureSelect XT HS2 DNA | | DreamPrep NGS | blue | Agilent
    Targeted enrichment    		 NGS SureSelect XT HS2 DNA      
    Illumina | NGS | Illumina DNA PCR Free Prep | | Veya NGS | blue | DreamPrep NGS | green | Illumina
    DNA-Seq    		 NGS Illumina DNA PCR Free Prep

    		 
    Illumina | NGS | Illumina DNA PCR-Free Prep | | DreamPrep NGS | green | Vendor | DreamPrep NGS Compact | blue | Illumina
    DNA-Seq    		 NGS Illumina DNA PCR-Free Prep   V    
    Illumina | NGS | Illumina DNA Prep | | Veya NGS | green | Tecan | DreamPrep NGS | green | Vendor | DreamPrep NGS Compact | blue | Illumina
    DNA-Seq    		 NGS Illumina DNA Prep T
    		V    
    Illumina | NGS | Nextera XT DNA Library Preparation Kit | | DreamPrep NGS | green | Tecan | DreamPrep NGS Compact | blue | Illumina
    DNA-Seq    		 NGS Nextera XT DNA Library Preparation Kit   T    
    Illumina | NGS | TruSeq DNA PCR-Free | | DreamPrep NGS | blue | Illumina
    DNA-Seq    		 NGS TruSeq DNA PCR-Free      
    Illumina | NGS | COVIDSeq Test (1 plate per run) | | DreamPrep NGS | green | Vendor | Illumina
    RNA-Seq    		 NGS COVIDSeq Test (1 plate per run)   V
    		 
    Illumina | NGS | COVIDSeq Test (2 plates per run) | | DreamPrep NGS | green | Vendor | Illumina
    RNA-Seq    		 NGS COVIDSeq Test (2 plates per run)   V
    		 
    Illumina | NGS | Illumina Stranded mRNA Prep | | DreamPrep NGS | yellow | DreamPrep NGS Compact | blue | Illumina
    RNA-Seq    		 NGS Illumina Stranded mRNA Prep      
    Illumina | NGS | Illumina Stranded Total RNA Prep with Ribo-Zero Plus | | Veya NGS | blue | DreamPrep NGS | yellow | Illumina
    RNA-Seq    		 NGS Illumina Stranded Total RNA Prep with Ribo-Zero Plus
    		   
    Illumina | NGS | Stranded Total RNA Prep with Ribo-Zero PLUS and PLUS Microbiome | mike | DreamPrep NGS | blue | Illumina
    RNA-Seq    		 NGS Stranded Total RNA Prep with Ribo-Zero PLUS and PLUS Microbiome      
    Illumina | NGS | TruSeq Stranded mRNA | | DreamPrep NGS | pink | Illumina
    RNA-Seq    		 NGS TruSeq Stranded mRNA      
    Illumina | NGS | TruSeq Stranded Total RNA Library Prep (Human/Mouse/Rat) | | DreamPrep NGS | blue | Illumina
    RNA-Seq    		 NGS TruSeq Stranded Total RNA Library Prep (Human/Mouse/Rat)      
    Illumina | NGS | 16S Metagenomic Sequencing | | DreamPrep NGS | blue | Illumina
    Targeted enrichment    		 NGS 16S Metagenomic Sequencing      
    Illumina | NGS | 16S Metagenomic Sequencing Library Preparation | mike | DreamPrep NGS Compact | blue | Illumina
    Targeted enrichment    		 NGS 16S Metagenomic Sequencing Library Preparation      
    Illumina | NGS | AmpliSeq for Illumina Custom DNA Panel | mike | DreamPrep NGS | blue | Illumina
    Targeted enrichment    		 NGS AmpliSeq for Illumina Custom DNA Panel      
    Illumina | NGS | AmpliSeq for Illumina Custom RNA Fusion Panel | mike | DreamPrep NGS | blue | Illumina
    Targeted enrichment    		 NGS AmpliSeq for Illumina Custom RNA Fusion Panel      
    Illumina | NGS | AmpliSeq Illumina Custom RNA Panel | mike | DreamPrep NGS | blue | Illumina
    Targeted enrichment    		 NGS AmpliSeq Illumina Custom RNA Panel      
    Illumina | NGS | Illumina DNA Prep with Enrichment | | Veya NGS | blue | DreamPrep NGS | yellow | DreamPrep NGS Compact | blue | Illumina
    Targeted enrichment    		 NGS Illumina DNA Prep with Enrichment
    		 
    Illumina | NGS | Illumina DNA Prep with Exome 2.0 Plus Enrichment | | Veya NGS | blue | DreamPrep NGS | yellow | Illumina
    Targeted enrichment    		 NGS Illumina DNA Prep with Exome 2.0 Plus Enrichment
    		   
    Illumina | NGS | Illumina RNA Prep with Enrichment (L) Tagmentation | | DreamPrep NGS | yellow | Illumina
    Targeted enrichment    		 NGS Illumina RNA Prep with Enrichment (L) Tagmentation      
    Illumina | NGS | TruSight Oncology 500 DNA/RNA High-Throughput Kit | | DreamPrep NGS | blue | DreamPrep NGS Compact | blue | Illumina
    Targeted enrichment    		 NGS TruSight Oncology 500 DNA/RNA High-Throughput Kit      
    Illumina | NGS | TruSight Oncology 500 DNA/RNA Kit | | DreamPrep NGS | orange | Illumina
    Targeted enrichment    		 NGS TruSight Oncology 500 DNA/RNA Kit      
    Illumina | NGS | TruSight Oncology 500 DNA/RNA with HRD | | DreamPrep NGS | blue | Illumina
    Targeted enrichment    		 NGS TruSight Oncology 500 DNA/RNA with HRD      
    Integrated DNA Technologies (IDT) | NGS | Lotus™ DNA Library Prep Kit | | DreamPrep NGS | blue | Integrated DNA Technologies (IDT)
    DNA-Seq    		 NGS Lotus™ DNA Library Prep Kit      
    Integrated DNA Technologies (IDT) | NGS | xGen™ cfDNA & FFPE DNA Library Preparation Kit | | DreamPrep NGS | blue | Integrated DNA Technologies (IDT)
    DNA-Seq    		 NGS xGen™ cfDNA & FFPE DNA Library Preparation Kit      
    Integrated DNA Technologies (IDT) | NGS | xGen™ NGS Hybridization Capture | | DreamPrep NGS | blue | Integrated DNA Technologies (IDT)
    DNA-Seq    		 NGS xGen™ NGS Hybridization Capture      
    Integrated DNA Technologies (IDT) | NGS | Archer™ VARIANTPlex™ Complete Solid Tumor panel | mike | DreamPrep NGS | blue | Integrated DNA Technologies (IDT)
    Targeted enrichment    		 NGS Archer™ VARIANTPlex™ Complete Solid Tumor panel      
    Invivoscribe® | NGS | LymphoTrack Dx Library | mike | DreamPrep NGS | blue | Invivoscribe®
    Targeted enrichment    		 NGS LymphoTrack Dx Library      
    Lexogen | NGS | QuantSeq 3’ mRNA-Seq V2 Library Prep Kit with UDI | | DreamPrep NGS | yellow | Lexogen
    RNA-Seq    		 NGS QuantSeq 3’ mRNA-Seq V2 Library Prep Kit with UDI      
    MGI | NGS | MGIEasy FS DNA Library Prep | | DreamPrep NGS | blue | MGI
    DNA-Seq    		 NGS MGIEasy FS DNA Library Prep      
    MGI | NGS | MGIEasy RNA Directional Library Prep Set | | DreamPrep NGS | blue | MGI
    RNA-Seq    		 NGS MGIEasy RNA Directional Library Prep Set      
    New England BioLabs (NEB) | NGS | NEBNext® Ultra™ II DNA | | DreamPrep NGS | blue | New England BioLabs (NEB)
    DNA-Seq    		 NGS NEBNext® Ultra™ II DNA      
    New England BioLabs (NEB) | NGS | NEBNext Ultra II Directional RNA Poly(A) mRNA Isolation | | DreamPrep NGS | blue | New England BioLabs (NEB)
    RNA-Seq    		 NGS NEBNext Ultra II Directional RNA Poly(A) mRNA Isolation      
    New England BioLabs (NEB) | NGS | NEBNext Ultra II Directional RNA rRNA depletion | | DreamPrep NGS | blue | New England BioLabs (NEB)
    RNA-Seq    		 NGS NEBNext Ultra II Directional RNA rRNA depletion      
    Oxford Nanopore Technologies (ONT) | NGS | Ligation Sequencing Kit XL V14 (SQK-LSK114-XL) | | DreamPrep NGS | green | Vendor | Oxford Nanopore Technologies (ONT)
    DNA-Seq    		 NGS Ligation Sequencing Kit XL V14 (SQK-LSK114-XL)   V    
    Oxford Nanopore Technologies (ONT) | NGS | Native Barcoding Kit 96 V14 (SQK-NBD114.96) | | DreamPrep NGS | green | Vendor | Oxford Nanopore Technologies (ONT)
    DNA-Seq    		 NGS Native Barcoding Kit 96 V14 (SQK-NBD114.96)   V    
    Oxford Nanopore Technologies (ONT) | NGS | Rapid sequencing DNA V14 (SQK-RBK114) | | Veya NGS | yellow | DreamPrep NGS | blue | DreamPrep NGS Compact | yellow | Oxford Nanopore Technologies (ONT)
    DNA-Seq    		 NGS Rapid sequencing DNA V14 (SQK-RBK114)

    		 
    Oxford Nanopore Technologies (ONT) | NGS | Midnight kit with ARTIC method for high-throughput SARS-CoV-2 sequencing | | DreamPrep NGS | blue | Oxford Nanopore Technologies (ONT)
    RNA-Seq    		 NGS Midnight kit with ARTIC method for high-throughput SARS-CoV-2 sequencing      
    PacBio | NGS | HiFi prep kit 96 | mike | DreamPrep NGS | green | Vendor | PacBio
    DNA-Seq    		 NGS HiFi prep kit 96   V
    		 
    Pacific Biosciences (PacBio) | NGS | HiFi Plex Prep Kit 96 | | Veya NGS | blue | Pacific Biosciences (PacBio)
    DNA-Seq    		 NGS HiFi Plex Prep Kit 96
    		   
    Pacific Biosciences (PacBio) | NGS | SMRTbell prep kit 3.0 | | DreamPrep NGS | blue | DreamPrep NGS Compact | green | Vendor | Pacific Biosciences (PacBio)
    DNA-Seq    		 NGS SMRTbell prep kit 3.0     V  
    QIAGEN | NGS | QIAseq® FX DNA | | DreamPrep NGS | blue | QIAGEN
    DNA-Seq    		 NGS QIAseq® FX DNA      
    QIAGEN | NGS | QIAseq Fastselect RNA | mike | DreamPrep NGS | blue | QIAGEN
    RNA-Seq    		 NGS QIAseq Fastselect RNA      
    Qiagen | NGS | REPLI-g WTA Single Cell Kit | mike | Veya NGS | yellow | DreamPrep NGS Compact | yellow | Qiagen
    Single cell RNA-seq    		 NGS REPLI-g WTA Single Cell Kit    
    QIAGEN | NGS | QIAseq DIRECT SARS-CoV-2 | | DreamPrep NGS | yellow | QIAGEN
    Targeted enrichment    		 NGS QIAseq DIRECT SARS-CoV-2      
    Quantabio | NGS | sparQ DNA Library Prep Kit | | DreamPrep NGS | blue | Quantabio
    DNA-Seq    		 NGS sparQ DNA Library Prep Kit      
    Roche | NGS | KAPA EvoPrep & KAPA EvoPlus V2 | | DreamPrep NGS | green | Vendor | Roche
    DNA-Seq    		 NGS KAPA EvoPrep & KAPA EvoPlus V2   V
    		 
    Roche | NGS | KAPA HyperPrep | | DreamPrep NGS | green | Vendor | DreamPrep NGS Compact | blue | Roche
    DNA-Seq    		 NGS KAPA HyperPrep   V
    		 
    Roche | NGS | KAPA® HyperPlus | | DreamPrep NGS | green | Vendor | DreamPrep NGS Compact | blue | Roche
    DNA-Seq    		 NGS KAPA® HyperPlus   V
    		 
    Roche | NGS | Roche KAPA HyperCap Evolved Workflow v5.0 | | DreamPrep NGS | green | Vendor | Roche
    DNA-Seq    		 NGS Roche KAPA HyperCap Evolved Workflow v5.0   V
    		 
    SOPHiA GENETICS | NGS | SOPHiA DDM™ capture-based Applications | mike | DreamPrep NGS | yellow | SOPHiA GENETICS
    Targeted enrichment    		 NGS SOPHiA DDM™ capture-based Applications      
    Twist Biosciences | NGS | Library Preparation EF 2.0 and Twist Universal Adapter System | | DreamPrep NGS | blue | Twist Biosciences
    DNA-Seq    		 NGS Library Preparation EF 2.0 and Twist Universal Adapter System      
    Twist Biosciences | NGS | Human Core Exome | | DreamPrep NGS | blue | Twist Biosciences
    Targeted enrichment    		 NGS Human Core Exome      
    Twist Biosciences | NGS | Twist Target Enrichment Standard Hybridization v1 Protocol | mike | DreamPrep NGS | blue | Twist Biosciences
    Targeted enrichment    		 NGS Twist Target Enrichment Standard Hybridization v1 Protocol      
    Twist Biosciences | NGS | Twist Target Enrichment Standard Hybridization v2 | | DreamPrep NGS | blue | Twist Biosciences
    Targeted enrichment    		 NGS Twist Target Enrichment Standard Hybridization v2      
    Verogen | NGS | ForenSeq DNA Signature Prep Kit | mike | Veya NGS | | Transferable from DreamPrep NGS Compact | DreamPrep NGS Compact | blue | Verogen
    Targeted enrichment    		 NGS ForenSeq DNA Signature Prep Kit   Transferable from DreamPrep NGS Compact  
    Watchmaker Genomics | NGS | Watchmaker DNA Library Prep Kits | mike | DreamPrep NGS Compact | blue | Watchmaker Genomics
    DNA-Seq    		 NGS Watchmaker DNA Library Prep Kits      
    Watchmaker Genomics | NGS | Watchmaker DNA Library Prep Kits | mike | DreamPrep NGS | blue | Watchmaker Genomics
    DNA-Seq    		 NGS Watchmaker DNA Library Prep Kits      
     

    Method available:

    Automated method developed according to the kit vendor’s published protocol. Protocol was tested using water and/or reagents with Tecan instruments.

     

    Method qualification in progress:

    Automated method developed according to the kit vendor’s published protocol. It is currently being tested with reagents and commercial samples by the vendor.

     

    Method qualified: V T C

    Automated method developed according to the kit vendor’s published protocol. Method was qualified using kit vendor’s guidelines, reagents and commercial samples. Libraries prepared with these methods meet the kit vendor’s specifications and/or perform comparably to those prepared manually. Sequenced libraries were assessed either by the vendor (V vendor qualified), by Tecan (T Tecan qualified) or by the customer (C customer qualified).

     

    Method custom developed:

    Automated method developed according to specific customer need. Libraries tested by the customer.




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    Tab 04 / Detection
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    Sensitive and fast quantification

    The Infinite® F Nano+ multimode reader offers absorbance and high sensitivity fluorescence reading modes in one compact instrument. It combines the high sensitivity of fluorescence assays and the speed provided by reading plate formats of up to 384 wells.

     

    NGS library quantification is a crucial step for a successful sequencing run. Accurately determining the number of sequenceable molecules is highly significant for new high capacity sequencers, such as the NovaSeq system.

    Tecan NuQuant technology

    Tecan NuQuant technology

    Tecan NuQuant® is a novel library quantification method that accurately measures molar library concentration without the need for separate fragment size analysis. This proprietary method uses a fluorescent label that is incorporated into the library molecules during library preparation. NuQuant is independent of library size, allowing library molarity to be directly measured using fluorometers, such as the Infinite F Nano+ multimode reader.

    Integrated library QC in less than six minutes

    NuQuant library quantification on the Infinite F Nano+ plate reader eliminates the need for additional manual qPCR or capillary electrophoresis steps, saving significant time, resources and valuable library material.

    After final purification of the DNA libraries in a 96-well plate on the Fluent ® Automation Workstation, the fluorescence of the libraries and standards is measured on the Infinite plate reader. The molarity determined for each library can then be used for balanced pooling and subsequent sequencing on Illumina instruments.

    Other quantification assays

    Other quantification assays

    Reader integration for quantification

    DNA quantification can be achieved by measuring an aliquot with a fluorescent dye, such as PicoGreen® or QuantiFluor™, or by measuring the absorbance of the entire DNA sample. DNA quantification and normalization workflows typically include the following steps

    • Set-up of quantification assay
    • Determination of the DNA concentration in a reader
    • Calculation of a dilution scheme to achieve the target concentration for each well
    • Dilution of the DNA samples to the desired concentration.




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    Tab 05 / Literature
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