Freedom EVOlyzer®

Freedom EVOlyzer^®

Effective ELISA automation from Tecan

The Freedom EVOlyzer provides an ideal solution for processing entire assay panels in a single run. Offering reliable pipetting performance for various sample types, including serum, stool, urine and saliva, it can easily adapt to varying throughputs while maintaining speed, precision and accuracy.

Overview

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The Freedom EVOlyzer platform enables automation of all common ELISA steps without compromising operator safety, including sample distribution, sample predilution, reagent pipetting, incubation, plate washing, optical density reading and analysis.

Economical

Continuous sample loading enables fast response to changing demands, offering higher throughput and time savings.
Choose between washable fixed tips and disposable tips to optimize productivity and minimize sample carry over.
Dynamic scheduling optimizes system usage and turnaround times.
Maintenance reports support laboratory quality management and reduce administrative efforts.

Precise

Optimized pipetting parameters for consistent processing of precious and difficult to pipette sample types.
Capacitive liquid level detection (cLLD) enables pre-checking of sample, reagent and buffer volumes, while clot detection ensures reliable sample aspiration.
Individually controlled temperature for each heated incubation slot.
The Freedom EVOlyzer uses the sophisticated Magellan™ data reduction software for all endpoint assay analyses, providing powerful capabilities, excellent data presentation, and outstanding graphical flexibility.

Safe

All ELISA processes are monitored by the integrated process control. Automatic barcode scanning tracks all samples and consumables after loading for complete process security.
The system offers simple operation, with reagent shopping lists, loading guides, easy assay selection and multilanguage software.
Once the ELISA protocols are started, the platform offers long walkaway times without user interventions, during which the Freedom EVOlution™ software automatically process and logs all events.
Safety panels and loading hatches are unlocked as needed to allow access to the worktable.

Freedom EVOlution™

Freedom EVOlution software enables easy set-up of sophisticated pipetting procedures for fully automated ELISAs on the Freedom EVOlyzer workstation.

No need to compromise

The Freedom EVOlyzer suits many different laboratory demands, from small-scale analysis of low sample numbers with multiple assays to high throughput screening of thousands of samples using a more limited range of assays.

No need to compromise The Freedom EVOlyzer suits many different laboratory demands, from small-scale analysis of low sample numbers with multiple assays to high throughput screening of thousands of samples using a more limited range of assays.
General	Freedom EVOlyzer 100	Freedom EVOlyzer 150	Freedom EVOlyzer 200
Worktable size	Max. 25 grids (rack positions)	Max. 40 grids (rack positions)	Max. 58 grids (rack positions)
Tip configuration	2 or 4 tips	4 or 8 tips	8 tips
Tip types	Fixed and disposable tips, with or without filter (200/1000 μl) Various combinations of fixed and disposable tips can be used
Ambient incubator slots	6 positions	6 or 12 positions	12 positions
Heated incubator slots	6 positions	6 or 12 positions	12 positions
Power supply	100-120 V, 50-60 Hz 600 VA	220-240 V, 50-60 Hz 1200 VA	220-240 V, 50-60 Hz 1200 VA
Sample input formats	10 to 16 mm diameter sample tubes, microplates, strip plates or deep-well plates
Dimensions H/W/D (mm)	910/1,520/800	910/1,890/800	910/2,490/800
Mass	210 kg	260 kg	290 kg
Maximum number of concurrent assays	Up to 15 assays per run	Up to 20 assays per run
Typical number of plates processed in parallel	4-6	5-9	Up to 12

Liquid Handling
Pipetting precision achievable	10 μl with ≤3.5 % (fixed tip, disposable tip 200 μl) 100 μl with ≤0.75 % (fixed tip, disposable tip 200 μl)
Liquid detection	Down to 50 μl of conductive liquid can be detected
Predilution options	Common, serial or exclusive predilution, as well as combined predilutions on one plate
Minimum dispensing volume	10 μl (fixed tip, disposable tip 200 μl) 25 μl (disposable tip 1,000 μl)
Maximum dispensing volume ** Volumes >900 μl require optimization by a trained application specialist	900 μl**

Barcode Identification: PosID™
Barcode identification	Sample tubes, microplates, deep-well plates, strip plates, reagent containers
Barcode evaluation	Sample identification, reagent identification, lot number control, lot expiry date control
Supported formats	Code 39, Code 128, Codabar and 2 of 5 interleaved

Incubator: MIO™
Temperature range	37 °C or 46 °C with accuracy of +/- 1°C
Shaker	Optional, up to 500 rpm

Washer: HydroFlex™
Manifold	8-way or 16-way
Wash channels	4 channels for wash buffers, with wash buffer capacities: 2 x 2 liters and 2 x 4 liters, with liquid level sensors
Wash mdoe	Strip or plate mode

Photometer: Sunrise™
Measurement range	340-399 nm, 0-3.0 OD; 400-750 nm, 0-4.0 OD
Wavelength selection	4 or 6 fixed interference filters (405, 450, 492, 540, 570, 620 nm) or gradient filter (400-700 nm)
Measurement time	Single wavelength: 6 seconds Dual wavelength: 8 seconds

Software: Freedom EVOlution™
Operating system	Microsoft Windows® 10 Enterprise LTSC, 64-bit
LIMS interface	Bi-directional ASTM (NCCLS LIS1, NCCLS LIS) interface

Brochures

Freedom EVOlyzer®

The Freedom EVOlyzer enables full automation of microplate-based ELISA analysis.

It is adaptable for varying throughputs while maintaining the speed, precision and accuracy needed to operate in a clinical environment.

The platform is easily integrated with LIMS solutions, via ASTM communication, and is well suited for dynamic environments, thanks to its continuous loading functionality.

Freedom EVOlyzer® 100

Entry-Level ELISA Automation.

The Freedom EVOlyzer 100 provides an ideal automation solution of microplate-based ELISA analysis for laboratories with low to intermediate throughput.

It offers precision and accuracy in a compact footprint.

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