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HydroFlex™ microplate washer

Cells. Beads. ELISAs.

The HydroFlex microplate washer is a truly flexible and upgradeable platform for automated microplate strip washing, magnetic bead washing and vacuum filtration in 96-well plate formats. It is an ideal solution for a wide range of cell-, bead- and ELISA-based applications, reflecting over 30 years of Tecan expertise in advanced liquid handling.

Integrated application flexibility

Every HydroFlex washer comes with microplate shaking and automated buffer switching included, ready for ELISA washing and gentle washing of cells. In addition, optional user-interchangeable plate carriers are available for easy switching between washing and filtration applications. For magnetic bead washing, the HydroFlex uses a patented carrier design with two neodymium magnets per well, ensuring efficient bead settling.

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Optional application modules, with user-exchangeable plate carriers for:

  • Automated washing of ELISAs and gentle washing of adherent cells
  • Efficient washing of Luminex-type magnetic beads (xMAP® assays) using a patented carrier design with 108 powerful neodymium magnets
  • Automated washing of small magnetic beads, such as Dynabeads® MyOne™, using an array of 96 extra powerful neodymium magnets
  • Automated low-level vacuum filtration system for processing of non-magnetic beads using filter-bottom plates
  • Automated high-level vacuum filtration system for post-PCR DNA purification

Selection of of universal wash heads, supporting all application modules:

  • Eight-way manifold for parallel washing of eight wells
  • 16-way manifold for parallel washing of 16 wells

Process control option for reliability and safety:

The optional Liquid Level Detection (LLD) system controls the fill levels in the wash buffer and waste bottles, warning the user when a critical liquid level has been reached.

Personalize your HydroFlex plate washer:

Choose your favorite color:

  • White (standard)
  • Metallic silver
  • Metallic red
  • Metallic blue
  • Metallic orange
  • Lime green

Ready for robotic integration:

Every HydroFlex plate washer is ready for integration onto the following automated platforms:

Highlights at a glance

  • Ready for automated washing of ELISAs, cells and protein arrays
  • Magnetic bead washing using powerful neodymium magnets
  • Two magnets per well for efficient bead settling (patented design)
  • Automated system for vacuum filtration using vacuum sensor
  • Individual vacuum settings for each filtration step within a protocol
  • Control of wash head positions and speed settings for gentle cell washing
  • Up to two aspiration points per well for minimal residual volumes
  • Simultaneous aspiration and dispensing for ELISA washing
  • Automated buffer switching and plate shaking capabilities
  • Controls up to four wash buffers
  • Ready for robotic integration
  • Tested reliability to minimize maintenance and service costs
  • IQ/OQ procedures available
  • Designed to meet the IVD-directive 98/79/EC and 2011/65/EU RoHS (reduction of hazardous substances)
  • Supports FDA 21 CFR Part 11 functionality together with HydroControl™ software

ELISA and cell washing*

Using adjustable wash settings and head positions for optimized results.

  • For gentle washing of adherent and weakly adherent cells
  • For efficient ELISA washing and minimum residual volumes

Magnetic bead washing

Using a patented design with two neodymium magnets per well.

  • For efficient bead settling and good bead retention
  • Linear plate shaking and strong dispensing for mixing of beads

Vacuum filtration to waste

Using tunable vacuum for PCR clean-up and bead washing.

  • Automation-friendly solution for self-sealing of filtration plates
  • Optimized vacuum settings for each filtration step within a protocol

HydroControl™ software

The HydroFlex can be fully programmed and operated as a standalone instrument using the built-in display and keypad, and features a selection of predefined cleaning protocols for easy maintenance.

Every HydroFlex system is also supplied with Tecan's intuitive HydroControl software, providing example wash programs and a predefined plate library, as well as a simulation mode for offline operation. Wash programs created in HydroControl can be quickly transferred to the on-board memory of the HydroFlex plate washer via a USB interface, using the download function.

 

Validated

  • The HydroFlex plate washer has been designed to meet IVD-D 98/79/EC when configured for ELISA washing*
  • In combination with the HydroControl software, HydroFlex provides 21 CFR Part 11 functionality, such as electronic records, electronic signatures, user administration and audit trail functions*

Application Notes

Using the Tecan HydroFlex for gentle washing of A431- and weakly adherent P815-cell lines in 96-well plate format.

Using Tecan’s patented-design carrier with two magnets per well with the HydroFlex plate washer.

Using the Tecan HydroFlex and magnetic plate carrier for fast purification of proteins from a cell lysate using magnetic beads.

Using Tecan’s HydroFlex microplate washer instead of typical manual wash procedures with a multichannel pipette.

Using the Tecan HydroFlex with the high-level vacuum filtration option.

Brochures

Your easy way to optimized results with Tecan’s HydroFlex and HydroSpeed™ plate washers.

The HydroFlex plate washer is a truly flexible and upgradeable platform for automated microplate strip washing and vacuum filtration in 96-well plate formats. It is an ideal solution for a wide range of cell-, bead- and ELISA-based applications.

The reliable microplate washer for processing of ELISAs and cells in 96-well plates.

Tecan Journal Articles

The Protein Interaction Screening Unit at the German Cancer Research Center, Heidelberg, has automated the co-immune precipitation method for investigating protein-protein interactions (PPIs) using Tecan’s HydroFlex washer and Infinite® F200 microplate reader, increasing throughput up to 100-fold compared to manual methods, and greatly accelerating PPI screening.

Scientists at Life Technologies' Dynal® facility in Oslo, Norway, are using Tecan's HydroFlex microplate washers in their R&D and QC departments for automated washing of Dynabeads® assays, reducing manual pipetting and improving consistency of results.

Researchers at the University of Modena and Reggio Emilia, Italy, have introduced automated washing for Enzyme-Linked ImmunoSpot (ELISpot) assays, using the HydroFlex microplate washer.

     
Display unit   Liquid crystal display with two rows of 16 digits
Keyboard   Four-key membrane keyboard
Number of dispensing channels   1, 2 or 4 channels
Manifold types   8- or 16-way for parallel processing of 8- or 16-wells
     
Volume of solution dispensed   50-3,000 µl in 50 µl steps (washing)
50-400 µl in 50 µl steps (dispensing)
Dispensing accuracy   ≤1.2 % typical**
(300 µl dispense volume, dispense rate: 3, 8-way manifold)
Dispensing uniformity CV   ≤2.5 % over the plate typical**
(300 µl dispense volume, dispense rate: 3, 8-way manifold)
Residual volume   Crosswise aspiration: ≤1.8 μl per well typical** (96-well, flat-bottom Greiner® Bio-One plate)
(asp. rate: 3; asp. time: 4 sec; head speed: 10 mm/sec; 8-way manifold)
Vacuum range   High-level vacuum filtration: -150 to -850 mBar absolute pressure
Low-level vacuum filtration: -50 to -150 mBar absolute pressure
Interface   USB
Shaking   Linear shaking; high, medium and low settings
     
Supply   Auto sensing for the settings
100-120 V or 220-240 V, 50/60 Hz
Consumption   65 VA
     
Outside dimensions for plate washing   Width: 27.5 cm; depth: 36.6 cm; height: 18.5 cm
Weight   6.6 kg
Color   White (standard),Metallic silver, Metallic red, Metallic blue, Metallic orange, Lime green
** Specifications are subject to change. Typical performance values represent the average observed factory tested values. For product specifications refer to operators manual.

* HydroFlex has been designed to meet 98/79/EC IVD-D when configured for ELISA washing. In combination with the HydroControl software, it also provides FDA 21 CFR part 11 functionality.