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| Sunrise™ | Infinite® F50 / Robotic | Infinite® M Nano | Infinite® Lumi | |
| Wavelength selection | ||||
| Filter-based instrument | ||||
| Tunable wavelength selection | Tunable gradient filter |
Quad4 monochromator™ |
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| Detection modes | ||||
| Absorbance endpoint & kinetics spectral range |
340-750 nm | 400-750 nm | 230-1000 nm | |
| Absorbance scans spectral range |
400-700nm | 230-1000nm | ||
| Absorbance multichannel detection | 12 channels |
8 channels |
1 channel |
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| Luminescence spectal range |
380-600 nm | |||
| Glow luminescence | ||||
| Flash luminescence | ||||
| Dual Color luminescence | ||||
| Plate formats | ||||
| Number of wells | 96 | 96 | 6-384 | 6-384 |
| NanoQuant Plate™ | ||||
| Cuvettes | ||||
| Further options | ||||
| Temperature control up 42°C | ||||
| Barcode scanner | ||||
| Upgradeable | ||||
| QC tools | ||||
| User interfaces | ||||
| Workflow oriented i-control™ software | ||||
| Magellan™ data analysis software | ||||
| Exemplified Applications | ||||
| ELISA | ||||
| Agglutination reading | ||||
| Nucleic acid quantification* | ||||
| Protein quantification | ||||
| Labelling efficency | ||||
| ATP detection | ||||
| Reporter assays | ||||
| Ca2+ release | ||||
| Ready for automation | ||||
| Connect™ plate stacker | ||||
| Freedom EVO® | ||||
| Fluent® | ||||
| Designed to meet regulatory needs | ||||
| 98/79/EC IVD-D | ||||
| 21 CFR Part 11 support** | ||||
| 2011/65/EU RoHS | ||||
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| Spark® Cyto* |
Spark |
Infinite® M Nano Plus |
Infinite® F Nano Plus |
Infinite® M Plex |
Infinite® F Plex |
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| Wavelength selection | ||||||
| Filter-based wavelength selection |
incl. 3+1 dichroics |
incl. 3+1 dichroics |
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| Quad4 monochromators™ (tunable wavelength) |
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| QuadX monochromators™ (tunable wavelength and bandwidth) |
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| Fusion™ optics (filter and Quad4/Quad X monochromators) |
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| Labware formats | ||||||
| Microplates | 1-1536 | 1-1536 | 6-384 | 6-384 | 6-384 | 6-384 |
| Cuvette adapter plate | ||||||
| Cuvette port | ||||||
| NanoQuant Plate™ compatible (2µl micro volume plate) |
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| Cell Chips™ cell counting slides | ||||||
| Detection modes | ||||||
| Absorbance endpoint + Kinetic spectral range |
200–1,000 nm | 200–1,000 nm | 230–1,000 nm | 230–1,000 nm | 230–1,000 nm | 230–1,000 nm |
| Absorbance spectral scan | ||||||
| Fluorescence top and bottom reading spectral range |
Ex 230-900 nm Em 280-900 nm |
Ex 230-900 nm Em 280-900 nm |
Ex 230–850 nm Em 280-850 nm |
Ex 230–850 nm Em 280-850 nm |
Ex 230-850 nm Em 280-850 nm |
Ex 230-850 nm Em 280-850 nm |
| Fluorescence spectral scan | ||||||
| Adjustable bandwidth in fluorescence | ||||||
| Automated z-focusing top | ||||||
| Automated z-focusing bottom | ||||||
| Fluorescence Polarization (FP) | ||||||
| Time-resolved fluorescence (TRF) | ||||||
| Fluorescence resonance energy transfer (FRET) |
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| Time-resolved fluorescence resonance energy transfer TR-FRET |
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| Multiple reads per well | ||||||
| Optimal read in fluorescence bottom | ||||||
| Well area scan (100x100 spots/well) |
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| Flash and glow luminescence incl. BRET | ||||||
| Luminescence spectral scan (tunable wavelength) |
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| AlphaScreen®/AlphaLISA®/AlphaPlex® | ||||||
| Automated cell counting in Cell chips™ | ||||||
| Brightfield | ||||||
| Digital phase contrast imaging | ||||||
| Fluorescence imaging | ||||||
| Incubation | ||||||
| Temperature control up to 42°C | ||||||
| Active cooling below ambient temperature (Te-Cool™) |
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| CO2 and O2 incubation option (GCM™ gas control module) |
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| Humidity control (evaporation protection) |
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| Further Options | ||||||
| Two reagent injectors | ||||||
| Injector with heater and stirrer | ||||||
| Automated Lidlifter™ | ||||||
| Barcode scanner | ||||||
| Upgradeable | ||||||
| IQ/OQ and QC tools | ||||||
| 21 CFR Part 11 support** | ||||||
| User interfaces | ||||||
| i-control™ / SparkControl™ Reader confirmed software |
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| Magellan™ data analysis software | ||||||
| Image Analyzer™ software | ||||||
| Ready for automation | ||||||
| Spark Stack | ||||||
| Freedom EVO® | ||||||
| Fluent® | ||||||
| Applications | ||||||
| Nucleic acid quantification | ||||||
| Protein quantification | ||||||
| ATP assays | ||||||
| Genereporter assays | ||||||
| BRET applications | ||||||
| HTRF® | ||||||
| DLReady™ | ||||||
| Transcreener® FI | ||||||
| Transcreener® FP | ||||||
| Transcreener® TR-FRET | ||||||
| Confluence assessment | ||||||
| Nuclei counting via Hoechst | ||||||
| GFP transfection efficiency | ||||||
| Cell viability via Calcein/PI | ||||||
| Cell death via Annexin V | ||||||