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PCR set-up

Automated PCR Set-up with targeted user interfaces

Automation of PCR set-up allows reaction volumes to be reduced while increasing throughput and reproducibility.

Related products:  Liquid handling and automation  |  Multimode readers
Miniaturized qPCR Set-up
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Tab 01 / Miniaturized qPCR Set-up
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Low volume qPCR set-up

The D300e Digital Dispenser offers significant sample, primer and reagent savings, and greatly reduces workflow times. Its dispensing precision allows accurate results to be achieved in very small reaction volumes, enabling scientists to perform more experiments with the same amount of samples and reagents.

Using the D300e’s PCR wizard, setting up qPCR reactions becomes a matter of minutes. In just four guided steps, you can create even complex plate layouts with standard curves and varying template concentrations. In addition, the exceptional low volume dispensing capabilities of the D300e allow you to reduce your total assay volumes.

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Accurate results in small reaction volumes

Samples, primers and master mixes were pipetted manually or using the D300e to create total reaction volumes of 5 or 12.5 µl in a 96 well plate.

Data points are average values of triplicate measurements, and error bars represent standard deviations.

D300e control software

Set up your PCR reaction in just 4 simple steps

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For research use only. Not for use in clinical diagnostic procedures.




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Tab 02 / Flexible PCR Set-up
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Effortless PCR set-up
for your everyday needs

The TouchTools™ PCR Wizard offers straightforward automation of PCR reaction set-up on Freedom EVO 100-200 instruments, using a high precision air displacement pipetting arm, or on a Freedom EVO 75 using the two-channel option with disposable tips.

The wizard simplifies reaction set-up for a wide range of applications – from endpoint, real-time and multiplex PCR protocols to sequencing, genotyping and gene expression methods, as well as pathogen and mutagenesis detection.

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Highlights

  • Straightforward software wizard guides users through instrument set-up and reduces training requirements
  • Fully automated reaction set-up for up to 96 master mixes and multiple PCR plates per run, including individual controls and dilution curves
  • High precision air displacement pipetting arm (Air LiHa) enables accurate liquid transfers from 0.5 µl to 1 ml
  • Seamless data transfer to Thermo, Bio-Rad and Roche real-time cyclers, avoiding transcription errors

Five step PCR set-up

Data input options

Plate layout optimization

Worktable loading

Progress of protocol

Seamless data transfer

Tecan Journal Articles

Genomics solutions for marine biology
[TJ 02/2015]

A Freedom EVO® platform is helping scientists at Italy’s Stazione Zoologica Anton Dohrn, one of the world’s top marine biology and ecology research institutions, to provide a range of automated genomics services for marine biologists.

A powerful combination for qPCR
[TJ 02/2012]

The Dittmer Lab at the University of North Carolina is investigating the biology of viral cancers using a Freedom EVO® 150 with an integrated LightCycler® 480 Real-Time PCR System. This versatile set-up provides automated qPCR-based analysis for viral gene and microRNA expression profiling, as well as viral load testing of clinical trial samples.




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Tab 03 / High troughput PCR Set-up
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Fluent® Laboratory automation solution

Fluent’s groundbreaking design delivers more capacity and increased speed for genomic workflows, particularly for automated reaction set-up. This innovative solution offers excellent precision, outstanding troughput and increased walkaway times.

  • Simpler workflow design with FluentControl™
    and Smart Commands
  • Device integration options including
    • 1/2D barcode scanners
    • Temperature-controlled devices
    • Plate reader
    • Plate sealer
    • Thermocyclers
    • Centrifuge
Fluent® Laboratory automation solution

Fluent highlights

Intuition built right in

Built-in touchscreen interface guides users through daily tasks for fast, consistent operation. Integrated user management, including method permissions, ensures secure protocols and optimizes selection for each user.

Save reagents and samples

Adaptive Signal Technology™, a new generation of liquid level sensing, enables the use of smaller sample volumes, minimizes the risk of errors and provides exceptional automated error handling.

Simply faster and more precise

Three independent, task specific arms move in parallel to simultaneously and efficiently automate methods. Sample preparation, labware movement to peripheral devices and plate-to-plate or reagent additions can all occur in parallel — maximizing performance.

  

For research use only. Not for use in clinical diagnostic procedures.




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Tab 04 / dPCR SET-UP​
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Enhancing Digital PCR Workflows with Fluent's Automation Capabilities

The Fluent workstation optimizes digital PCR processes with its advanced automation, streamlining critical stages, including reaction assembly and sample mixing before partitioning, guaranteeing:

  • Fast and accurate liquid handling, ensuring precision in sample and reagent dispensing, while minimizing pipetting times.
  • Walkaway PCR sample preparation, reducing hands-on time. Let the Fluent do the pipetting for you.
  • Improved throughput and processing time, making digital PCR preparation more efficient and error-free.

Revolutionizing Nucleic Acid Quantification: The Power of Digital PCR (dPCR)

Digital PCR (dPCR) is a powerful molecular biology technique that revolutionizes the precise quantification of DNA or RNA molecules in a sample. It directly counts individual nucleic acid molecules based on on/off signals, providing absolute quantification data without the need for a standard curve, which is always required in other quantitative PCR methods. In dPCR, the target DNA or RNA is divided into thousands of minute partitions, each of which undergoes individual PCR amplification. Tecan offers comprehensive support for all pre-partitioning steps, including accurate liquid handling, reaction assembly, and sample mixing, ensuring a seamless dPCR workflow.

Digital PCR is invaluable in applications such as rare mutation detection, absolute quantification of nucleic acids, and copy number variation analysis.

Dye-labeled probes are employed during amplification to detect sequence-specific targets, with color intensity directly correlating with the amount of target DNA present. Simultaneous analysis of multiple targets, or multiplexing, is possible by dPCR. Two main dPCR technologies exist:

Droplet-based

dPCR_droplet_4_720x200

 

Microwell/microarray-based

dPCR_microarray_2_720x200

This innovative technology plays an instrumental role in various research areas and diagnostic applications, offering a faster and more accurate alternative to conventional qPCR. Researchers can rely on Fluent to optimize their digital PCR workflows, improving the accuracy and efficiency of their genomic analyses.

Expanding Digital PCR Applications for Our Customers

Tecan's commitment to excellence extends beyond our own capabilities, as we actively collaborate with various partners to optimize PCR workflows for our customers. This collaborative approach enables us to deliver optimized set-ups for PCR, including digital PCR. By working closely with these partners, we ensure that our customers have access to cutting-edge applications, robust automation, and tailored solutions that address their specific needs.




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Tab 05 / Thermocycler integration
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Inheco On-Deck Thermocycler integration

Automate more of your genomics workflow – including reaction set-up for endpoint PCR or qPCR, hybridization and Sanger or next generation sequencing – with a dedicated on-deck thermocycler (ODTC®) from INHECO. This cycler combines an ultra-compact design with exceptional thermal performance for faster cycling times. It can be flexibly integrated into the Freedom EVO to suit your workflow automation needs.

Top thermal performance

  • Patent pending vapor chamber (VCM®) technology
  • Rapid heat transfer for faster cycling times
  • Excellent well-to-well uniformity
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Minimal footprint

PCR cycling methods can be set up with INHECO’s script editor software and selected for a specific run using Freedom EVOware®. After the start of a run, Freedom EVOware drives the opening and closing of the ODTC and starts the PCR method for up to 10 integrated ODTC devices.

  • Most compact thermal cycler on the market
  • Flexible integration options
PCR_Set-up_SILA_Card_453x234.png

SiLA compliant

The INHECO ODTC uses the SiLA standard for software control, and can be controlled by the Freedom EVOware SiLA driver, V1.1 or higher.

  

For research use only. Not for use in clinical diagnostic procedures.




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Tab 06 / Literature
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Brochures

Tecan D300e Digital Dispenser

Repetitive manual pipetting and tedious serial dilutions for drug discovery assays just became a thing of the past. With Tecan’s D300e Digital Dispenser, you can automatically dispense any volume into any well of any assay plate, quickly and easily. Simply add your liquid to the Dispensehead and let the instrument do the rest.

Freedom EVO® PCR workstation

The TouchTools™ PCR Wizard offers straightforward automation of PCR reaction set-up on the Freedom EVO® PCR workstation, using the high precision Air displacement pipetting arm (Air LiHa).

Complex PCR set-up simplified

Fully automated reaction set-up for complex PCR plate layouts, with up to 96 master mixes and multiple PCR plates per run, plus individual controls and dilution curves.

Fluent® Laboratory Automation Solution

Fluent’s groundbreaking design delivers more capacity and increased speed for genomic workflows. This innovative solution offers superior precision, higher throughput and longer walkaway times. Get more done in less time, with greater confidence.

Integration of thermocycler

Automate more of your genomics workflows – including reaction set-up for endpoint PCR or qPCR, hybridization, Sanger or next generation sequencing – with a dedicated on-deck cycler from INHECO.

Application Notes

qPCR setup with KAPA SYBR FAST on Fluent

This Application Note describes automated qPCR set-up with the KAPA™SYBR® FAST qPCR Kit on the Fluent® Automation Workstation with minimal hands-on time. In the TouchTools™ user interface, the number of samples, master mixes or replicates can be chosen from a drop-down menu or input manually. Optional procedures include generation of a standard curve with a variable number of dilution points. Typical set-up times are 25 min for a 96-well plate.

Effortless PCR set-up with the TouchTools PCR Wizard

DNA profiling of endangered species by PCR at the Institute for Environmental Protection and Research.

Improving qPCR set-up

The application note shows that the D300e is a suitable tool for qPCR set-up, offers significant sample, primer and reagent savings, and greatly reduces workflow time.

Miniaturizing qPCR reactions

The data presented in this application note demonstrates that the D300e can be used to miniaturize qPCR reactions to volumes as low as 2 μl and in addition offers greater process security and speed of the automated set-up.
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