Automated Buildup of Biomimetic Films in Cell Culture Microplates for High-Throughput Screening of Cellular Behaviors

July 1, 2018

LbL films were directly deposited in 96‐well cell culture microplates (Greiner Bio‐One, Germany) using an automated liquid handling machine (TECAN Freedom EVO 100, Tecan France, Lyon).

An automatic method is established for layer-by-layer (LbL) assembly of biomimetic coatings in cell culture microplates using a commercial liquid-handling robot. Highly homogeneous thin films are formed at the bottom of each microwell. The LbL film-coated microplates are compatible with common cellular assays, using microplate readers and automated microscopes. Cellular adhesion is screened on crosslinked and peptide-functionalized LbL films and stem cell differentiation in response to increasing doses of bone morphogenetic proteins (2, 4, 7, 9). This method paves the way for future applications of LbL films in cell-based assays for regenerative medicine and high-throughput drug screening. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Machillot, P; Quintal, C; Dalonneau, F; Hermant, L; Monnot, P; Matthews, K; Fitzpatrick, V; Liu, J; Pignot-Paintrand, I; Picart, C;

Journal: Adv. Mater. Weinheim Pages: e1801097

Original article (29786885)