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Protein detection and analysis

Tecan offers a variety of sophisticated solutions for protein analysis. From robust multimode readers to the integrated label-free analysis using the FortéBio Octet® platform to protein digestion and MALDI spotting, we can streamline your assay development process.




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Tab 01 / Detection
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Excellent performance in detection

Tecan’s microplate readers enable protein quantification and analysis at the touch of a button, and can be easily integrated in liquid handling platforms. Multimode readers offer a choice of detection modes to suit individual needs, with a modular design allowing them to be upgraded as laboratory needs change.

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Our recommendation for proteins

The Infinite® 200PRO is our most popular instrument for protein detection and analysis.

Exceptional format flexibility and fast read times

  • 6- to 384-well plates
  • NanoQuant Plate™ – dedicated low volume quantification solution
  • 96 wells in ~20 sec
  • 384 wells in ~30 sec

Broad range of assays

  • Protein quantification (BCA, Bradford and Lowry)
  • Protein-protein binding
  • Receptor-ligand interaction
  • Many more

Add Magellan™ for a complete software solution

  • Kinetic analysis
  • All major curve fittings
  • Plate- to plate quality control




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Tab 02 / Label-free
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Label-free, real-time analysis

Empower your biotherapeutic development and drug discovery with Tecan and FortéBio – automated high throughput Dip and Read™ biosensor assays using the Octet® platform.

Fully automate sample and assay preparation, to allow label-free, real-time interaction analysis in 96- and 384-well microplate formats.

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Effortless screening of

  • Proteins
  • Antibodies
  • Peptides
  • DNA and RNA
  • Small molecules

Highlights

  • Dip and Read technology avoids the need for pipetting, allowing even very crude materials to be tested
  • Standard off-the-shelf solutions consists of hardware, software and plate handling accessories
  • Tailored to your needs on Freedom EVO® 100, 150 or 200 platforms

Integrated with up- and downstream processes

  • Expression screening, eg. MultiTrap™
  • Protein purification, eg. RoboColumns®
  • Nucleic acid purification

…and more




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Tab 03 / maldi
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Convenient and robust in-gel protein digestion

The Freedom EVO® Protein Digest workstation enables fully automated protein digestion for subsequent MALDI-TOF analysis, including destaining of gel plugs, reduction, alkylation, in-gel trypsin digestion, peptide extraction and spotting onto MALDI targets. The system allows up to 192 samples to be processed a day, including MALDI spotting.

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Highlights

  • No piercing of gel-plugs, due to contactless liquid handling
  • Maximized protection against keratin contamination using contactless pipetting
  • Minimized evaporation during incubation steps
  • Efficient drying processes
  • Overnight running, by cooling the peptide mixture after digestion

Reliable sequence coverage

  • Robust and repeatable results
  • Sequence coverage equal to or better than manual processes
  • Excellent sensitivity down to 50-100 fmol of protein per gel plug

Decreased hands-on time

Both automated and manual in-gel digest protocols need approximately one hour of preparation (green bar), but automation frees the user from additonal hands-on time (red bars) over a seven hour period.

Data kindly provided by Max-Planck-Institute of Experimental Medicine in Göttingen, Germany (see Jahn et al., Anal. Bioanal. Chem. 2006, 386:92-103)

Automated desalting and MALDI-spotting

Eliminating salt from peptide samples obtained from 1D- or 2D-gel protein digestion is essential for successful MS analysis.

Fully automated peptide desalting and MALDI target spotting using ZipTip® columns (Merck) on Tecan liquid handling platforms. Various sizes of MALDI targets from different manufacturers can be accommodated, allowing high throughput, error-free spotting.

Highlights

  • Precise positioning of Liquid Handling Arm on MALDI targets
  • Spotting on different MALDI-Targets
  • Integration with protein digest workstation for a 2-in-1 solution
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Tab 04 / Literature
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Tecan Journal Articles

The proteomics group at the Institute for Systems Biology, Seattle, is using a Freedom EVO to generate samples for analysis by mass spectrometry, providing the reproducibility and throughput to enable a range of different projects to be run entirely in house.

Seeing is believing
[TJ 01/2011]

Scientists at the Center for Integrated Protein Science Munich have taken advantage of the 3D scanning capabilities of the Infinite® M1000 microplate reader for the study of epigenetics.

Integration of the FortéBio Octet® 384 platform on the Freedom EVO® workstation offers automated, real-time, label-free assays to support bioprocessing and drug discovery workflows. This robust, off-the-shelf integration provides automated liquid handling for assay plate set-up and increases walkaway times.

The Proteomics Group at the Max Planck Institute, Göttingen, has collaborated with Tecan to develop an innovative automated solution for in-gel digestion in large-scale proteome analyses.

For research use only.