Infinite® 200 PRO multimode reader- Typical performance values

Light Source UV Xenon flashlamp
Wavelength selection
Infinite M200 PRO
Quad4 Monochromators system
(2 excitation and 2 emission monochromators)
Bandwidth Ex: < 5 nm for λ ≤ 315 nm and < 9 nm for λ > 315 nm; Em: < 20 nm
  Absorbance Fluorescence
Wavelength accuracy < ± 0,5 nm for λ > 315 nm;
< ± 0,3 nm for λ ≤ 315 nm
< ± 2 nm for λ > 315 nm;
< ± 1 nm for λ ≤ 315 nm
Wavelength reproducibility < ± 0,5 nm for λ > 315 nm;
< ± 0,3 nm for λ ≤ 315 nm
< ± 1 nm for λ > 315 nm;
< ± 0,5 nm for λ ≤ 315 nm
Infinite F200 PRO
Up to 4 filter pairs per slide
Wavelength range Standard
Fluorescence intensity Ex 230 – 850 nm, Em 280 – 850 nm
Absorbance 230 - 1000 nm
Detectors  Fluorescence – PMT, UV and red-sensitive
Absorbance – UV silicon photodiode
Luminescence – photon counting system with low dark current PMT
Plate formats 6- to 384- well plates, cuvettes, NanoQuant Plate
Temperature control Ambient +5°C up to 42°C
Shaking Linear, orbital
Fluorescence sensitivity1) values Infinite F200 PRO Infinite M200 PRO
Fluorescence top reading1) 85 amol / well
(0,85 pM, 384-well plate)
170 amol / well
(1,7 pM; 384-well plate)
Fluorescence bottom reading1) 5,0 fmol / well
(25 pM, 96-well plate)
9,0 fmol / well
(45 pM; 96-well plate)
TRF2) 2,8 amol / well
(28 fM; 384-well plate)
 90 amol / well
(0,9 pM; 384-well plate)
Luminescence sensitivity values Standard  
Glow luminescence3) 225 amol ATP / well
(9 pM; low volume 384-well plate)
 
Flash luminescence4) 12 amol ATP / well
(218 fM; 384-well plate)
 
Absorbance
Ratio accuracy 260 / 280 nm ± 0,07
Precision @ 260 nm < 0,2 %
Accuracy @ 260 nm < 0,5 %
Measurement range 0 - 4 OD
Injectors
Pump speed 100 - 300 µl/s
Injection volume selectable in 1 μl increments; max. volume: 800 μl per stroke
Dead volume 100 µl including pump back
Fastest Read Times
96 well plate 20 sec
384 well plate 30 sec
Wavelength Ex / Em-scan,
96 well plate 450 – 550 nm
5 nm step
150 sec

1)  Detection limit for Flourescein
2)  Detection limit for Europium
3)  Detection limit for ATP (144-041 ATP detection kit SL (BioThema))
4)  Detection for ATP (ENLITE® Kit)


*  Specifications are subject to change. Performance values represent the average observed factory tested values.For product specifications refer to operators manual.

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