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    Biobanking

    ALIQUOTING, PREPARATION AND FORMATING OF BIOLOGICAL SAMPLES FOR LONG TERM STORAGE

    Tecan offers fully automated solutions for pre-analytical sample handling based on the versatile Freedom EVO® platform, with the flexibility to fit into your existing laboratory workflow while taking care of the most labor-intensive and error-prone procedures.

    Related products:  Freedom EVO®  |  Tecan Integration Group (TIG)  |  Consumables

    Barcode reading

    Arriving samples are scanned after entering the system in racks and barcodes can be communicated to the LIS/LIMS to retrieve work orders or simply confirm arrival of the tube in the laboratory and create an inventory of samples.

    All common 1D barcodes can be read within the strip rack or 2D barcodes on a single tube when transporting it to the first module after entering the system.

    1D primary tube scanning

    1D/2D individual tube scanning

    2D SBS rack with 1D plate barcode

    Centrifugation

    The integrated centrifuge guarantees consistent centrifugation quality for all samples that require centrifugation.

    Time, temperature, speed and acceleration and deceleration profiles can be set for each run individually provided a consistent requirement for the samples.

    Pre-centrifuged samples can be centrifuged again or bypass the centrifuge and move on to the following procedure.

    De-capping

    To maximize safety for your staff and increase process security, the onboard decapper takes caps and plugs off most commonly used primary tubes.

    The cap type is detected and the tube taken away from the worktable to avoid cross contaminating other samples or the environment in the system. An protective shield applied during the decapping step prevents any aerosols from entering the area where samples are handled on the worktable.

    De-capping & re-capping

    Integration of a compact, high-speed whole rack tube capper/decapper that can automatically remove, then recap or dispose of, TPE septum caps from a full 96 tube rack, eliminates the need to manually remove the caps, dramatically improves productivity.

    The FluidiX XDC-96 will also dispose of the caps or recap the same caps back into the same tubes. Manually recapping tubes after sampling is no longer required.

    Cycle time (decapping and recapping) is 15-30 seconds, depending on tube type. Compatible with most 96 format TPE septum sealed caps (0.5ml, 0.75ml, 1.0ml and 1.4ml).

    Tube Inspection & Fractionation

    The tube inspection unit with the patented “see through” feature reads the liquid level and determines the location of the separation between serum and separation gel or red blood cells. To maximize the reliability, it also features a rotating device to increase its ability to process all tubes presented to it. Based on the values provided by the tube inspection unit, the volume available can be calculated and subsequent pipetting can be performed most efficiently.

    After identification of the separation interface between the red blood cells and buffy coat/plasma by the tube inspection unit, the sample can be fractionated into plasma aliquots and/or buffy coat.

    Buffy Coat & PBMC Isolation

    Tecan, in collaboration with the IBBL, has developed a method that uses spiral pipetting for highly efficient extraction of buffy coat.

    The Application Note Efficient buffy coat extraction explains how this method increases yields and reproducibility over the manual method.

    Tecan Journal Articles

    Tecan and the Integrated BioBank of Luxembourg (IBBL) have successfully co-developed an automated platform for buffy coat extraction based on a Freedom EVO® 200 workstation, relieving scientific staff from this time-consuming task and significantly increasing the yield of DNA.

    Since its inception in 2008, IBBL has been a biobanking pioneer, developing a high quality repository of samples to support biomedical research into a variety of diseases.

    Using a Freedom EVO® workstation, the center has developed an automated workflow for the processing and cryopreservation of a wide range of biological materials, and has recently added viable peripheral blood mononuclear cells to its standard whole blood extraction portfolio.

    Reformating & aliqoting

    Pipeting into a secondary tube, or into micro plates is performed by a flexible channel arm with disposable tips or, if the process allows it, by fixed needles.

    The arm configuration can vary between 2/4/8 channels which can be individually configured to carry fixed or disposable tips.

    In addition to the capacitive liquid level detection for volume calculation, clot detection and liquid tracking during the aspiration, on the Freedom Evo based liquid handling arm, the MultiSense technology allows pressure monitored pipetting and a qualitative liquid arrival check.

    Tecan Journal Articles

    University Hospital Regensburg has taken advantage of the open architecture of the Freedom EVO® workstation, creating a seamless connection between its routine diagnostics and biobanking activities.

    Working in partnership with the hospital’s Institute for Laboratory Medicine and Transfusion Medicine and diagnostic instruments provider, the Tecan Integration Group has developed a bespoke solution which offers fully automated preparation of samples for both biobanking and offline secondary testing.

    The Heidelberg CardioBiobank – part of Heidelberg University Hospital – collects thousands of patient samples every year for cryostorage and use in national and European medical research projects.

    Brochures

    Aliquoting, preparation and formating of biological samples for long term storage.

    Efficient gDNA extraction with the Promega ReliaPrep™ Large Volume HT gDNA Isolation system - tailored to your DNA biobanking needs.

    Application Notes

    Automated quality control for NGS Library Preparation and CRISPR/CAS9 Gene Editing

    Low volume DNA qualification by absorption of native nucleic acid or protein samples with an integrated Trinean Dropsense 96 UV/VIS droplet reader.

    Combined with its unique microfluidic 96well 2μl DropPlates, micro volume quantification is made possible in a fully automated workflow.

    Reliable extraction of buffy coat from human blood

    TIG Project Flyers

    Aliquoting of plasma/serum samples after detection of the separation layer with the Tecan Tube Inspection Unit.

    Aliquoting of blood, serum, plasma and urine from primary collection tubes into 96-screwcap-tube racks.

    Aliquoting and reformatting of blood and urine samples for long term storage.

    Aliquoting directly from SIEMENS® ADVIA® LabCell® Automation Solution.

    DNA extraction from blood with integrated Promega Maxwell 16, DNA quantitation on Trinean Dropsense 96 and subsequent DNA normalization.